Scanning

Electron

Microscopic

Observations

of Polyacrylamide

Received Structure\

of

microscope.

November

polyacrylamide

Structures

gel?

observed

\tantiated by different experiments state of hydration. Parameter\ gels have been varied to reveal structural polymerization

15.

patterns and

Gels

1974:

accepted

have

after

hrcn

Mav

vicwcd

freeze-drying

1. 1975 in

a scanning

of the

gels

as the preexisting structure of content and polymerization their effects on the observed

revealed by electron the molecular+ieving

microscopy properties

electron

have

been

huh-

of the gels in their of polyacrylamide morphology. The

suggest of thc\c

a model gel\.

of both

the

Polyacrylamide gels have been widely used in electrophoretic separation techniques and gel filtration since the initial findings of Raymond and Weintraub ( I ) and Ornstein (2) in 1959. The use of these gels has been broadened by the development of discontinuous electrophoresis systems (2), the use of sodium dodecyl sulfate (SDS) for protein electrophoresis (3). and the first publication of gel electrofocusing by Dale and Latner in 1968 (4). In spite of the wide use of polyacrylamide gels, the authors have so far found no investigation concerning the structure of the sieving gel.” MATERIALS

AND

METHODS

Acrylamide. N,N’-methylene-bisacrylamide (bisacrylamide, the crosslinker), and N.N.N’,N’-tetramethylethylenediamine (TEMED) were all obtained from Eastman Kodak Company (Rochester. NY); ammonium persulfate was purchased from Fisher Scientific Company (Fair Lawn, NY). Cyanogum. from American Cyanamid Company (Pearl River, NY). Durcopan was purchased from Fluka A.G. (Buchs SC. Switzerland), horse ferritin was obtained from Cappel Laboratories (Downingtown. PA), bovine serum albumin from Armour Laboratories (Kan’ Reinhard Riichel Germany. ’ While this paper Blank

/Ill’? Copyright All

rights

is yupportcd was

and Reimschuesael i1.1v11/011) 254, IO‘!).

I

Scanning electron microscopic observations of polyacrylamide gels.

Scanning Electron Microscopic Observations of Polyacrylamide Received Structure\ of microscope. November polyacrylamide Structures gel? ob...
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