Scanning
Electron
Microscopic
Observations
of Polyacrylamide
Received Structure\
of
microscope.
November
polyacrylamide
Structures
gel?
observed
\tantiated by different experiments state of hydration. Parameter\ gels have been varied to reveal structural polymerization
15.
patterns and
Gels
1974:
accepted
have
after
hrcn
Mav
vicwcd
freeze-drying
1. 1975 in
a scanning
of the
gels
as the preexisting structure of content and polymerization their effects on the observed
revealed by electron the molecular+ieving
microscopy properties
electron
have
been
huh-
of the gels in their of polyacrylamide morphology. The
suggest of thc\c
a model gel\.
of both
the
Polyacrylamide gels have been widely used in electrophoretic separation techniques and gel filtration since the initial findings of Raymond and Weintraub ( I ) and Ornstein (2) in 1959. The use of these gels has been broadened by the development of discontinuous electrophoresis systems (2), the use of sodium dodecyl sulfate (SDS) for protein electrophoresis (3). and the first publication of gel electrofocusing by Dale and Latner in 1968 (4). In spite of the wide use of polyacrylamide gels, the authors have so far found no investigation concerning the structure of the sieving gel.” MATERIALS
AND
METHODS
Acrylamide. N,N’-methylene-bisacrylamide (bisacrylamide, the crosslinker), and N.N.N’,N’-tetramethylethylenediamine (TEMED) were all obtained from Eastman Kodak Company (Rochester. NY); ammonium persulfate was purchased from Fisher Scientific Company (Fair Lawn, NY). Cyanogum. from American Cyanamid Company (Pearl River, NY). Durcopan was purchased from Fluka A.G. (Buchs SC. Switzerland), horse ferritin was obtained from Cappel Laboratories (Downingtown. PA), bovine serum albumin from Armour Laboratories (Kan’ Reinhard Riichel Germany. ’ While this paper Blank
/Ill’? Copyright All
rights
is yupportcd was
and Reimschuesael i1.1v11/011) 254, IO‘!).
I
Electron
Microscopic
Observations
of Polyacrylamide
Received Structure\
of
microscope.
November
polyacrylamide
Structures
gel?
observed
\tantiated by different experiments state of hydration. Parameter\ gels have been varied to reveal structural polymerization
15.
patterns and
Gels
1974:
accepted
have
after
hrcn
Mav
vicwcd
freeze-drying
1. 1975 in
a scanning
of the
gels
as the preexisting structure of content and polymerization their effects on the observed
revealed by electron the molecular+ieving
microscopy properties
electron
have
been
huh-
of the gels in their of polyacrylamide morphology. The
suggest of thc\c
a model gel\.
of both
the
Polyacrylamide gels have been widely used in electrophoretic separation techniques and gel filtration since the initial findings of Raymond and Weintraub ( I ) and Ornstein (2) in 1959. The use of these gels has been broadened by the development of discontinuous electrophoresis systems (2), the use of sodium dodecyl sulfate (SDS) for protein electrophoresis (3). and the first publication of gel electrofocusing by Dale and Latner in 1968 (4). In spite of the wide use of polyacrylamide gels, the authors have so far found no investigation concerning the structure of the sieving gel.” MATERIALS
AND
METHODS
Acrylamide. N,N’-methylene-bisacrylamide (bisacrylamide, the crosslinker), and N.N.N’,N’-tetramethylethylenediamine (TEMED) were all obtained from Eastman Kodak Company (Rochester. NY); ammonium persulfate was purchased from Fisher Scientific Company (Fair Lawn, NY). Cyanogum. from American Cyanamid Company (Pearl River, NY). Durcopan was purchased from Fluka A.G. (Buchs SC. Switzerland), horse ferritin was obtained from Cappel Laboratories (Downingtown. PA), bovine serum albumin from Armour Laboratories (Kan’ Reinhard Riichel Germany. ’ While this paper Blank
/Ill’? Copyright All
rights
is yupportcd was
and Reimschuesael i1.1v11/011) 254, IO‘!).
I
