Accepted Manuscript Selenium-enriched Agaricus bisporus increases expression and activity of glu‐ tathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon Tebo Maseko, Kate Howell, Frank R. Dunshea, Ken Ng PII: DOI: Reference:
S0308-8146(13)01339-3 http://dx.doi.org/10.1016/j.foodchem.2013.09.074 FOCH 14702
To appear in:
Food Chemistry
Received Date: Revised Date: Accepted Date:
24 June 2013 1 August 2013 12 September 2013
Please cite this article as: Maseko, T., Howell, K., Dunshea, F.R., Ng, K., Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon, Food Chemistry (2013), doi: http://dx.doi.org/10.1016/j.foodchem.2013.09.074
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Selenium-enriched Agaricus bisporus increases expression and activity of
2
glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon
3 4
Tebo Maseko, Kate Howell, Frank R. Dunshea, Ken NG*.
5
Department of Agriculture and Food Systems, Melbourne School of Land & Environment, The
6
University of Melbourne, Parkville, Victoria 3010, Australia.
7 8
________________________________________________________________________________
9
ABSTRACT
10
The effect of dietary supplementation with Se-enriched Agaricus bisporus on cytosolic
11
gluthathione peroxidase-1 (GPx-1), gastrointestinal specific glutathione peroxidase-2 (GPx-2),
12
thioredoxin reductase-1 (TrxR-1) and selenoprotein P (SeP) mRNA expression and GPx-1 enzyme
13
activity in rat colon was examined. Rats were fed for 5 weeks with control diet (0.15 µg Se/g feed)
14
or Se-enriched diet fortified with selenised mushroom (1 µg Se/g feed). The mRNA expression
15
levels were found to be significantly (P
S0308-8146(13)01339-3 http://dx.doi.org/10.1016/j.foodchem.2013.09.074 FOCH 14702
To appear in:
Food Chemistry
Received Date: Revised Date: Accepted Date:
24 June 2013 1 August 2013 12 September 2013
Please cite this article as: Maseko, T., Howell, K., Dunshea, F.R., Ng, K., Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon, Food Chemistry (2013), doi: http://dx.doi.org/10.1016/j.foodchem.2013.09.074
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
Selenium-enriched Agaricus bisporus increases expression and activity of
2
glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon
3 4
Tebo Maseko, Kate Howell, Frank R. Dunshea, Ken NG*.
5
Department of Agriculture and Food Systems, Melbourne School of Land & Environment, The
6
University of Melbourne, Parkville, Victoria 3010, Australia.
7 8
________________________________________________________________________________
9
ABSTRACT
10
The effect of dietary supplementation with Se-enriched Agaricus bisporus on cytosolic
11
gluthathione peroxidase-1 (GPx-1), gastrointestinal specific glutathione peroxidase-2 (GPx-2),
12
thioredoxin reductase-1 (TrxR-1) and selenoprotein P (SeP) mRNA expression and GPx-1 enzyme
13
activity in rat colon was examined. Rats were fed for 5 weeks with control diet (0.15 µg Se/g feed)
14
or Se-enriched diet fortified with selenised mushroom (1 µg Se/g feed). The mRNA expression
15
levels were found to be significantly (P
