This article was downloaded by: [Michigan State University] On: 02 January 2015, At: 20:50 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Selenium residues in sheep meat a
J.B. Stephenson & A.B. Grant
a
a
Research Division, Ministry of Agriculture and Fisheries , Wallaceville Animal Research Centre , Private Bag, Upper Hutt, New Zealand Published online: 23 Feb 2011.
To cite this article: J.B. Stephenson & A.B. Grant (1979) Selenium residues in sheep meat, New Zealand Veterinary Journal, 27:11, 232-232, DOI: 10.1080/00480169.1979.34659 To link to this article: http://dx.doi.org/10.1080/00480169.1979.34659
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
VOL. 27
NEW ZEALAND VETERINARY JOURNAL
232
Selenium residues in sheep meat J. B. Stephenson· and A. B. Grant·
N.Z. vet.J.27: 232 ABSTRACT
Selenium did not exceed lOS ng/g in muscle after single subcutaneous doses of 5 mg selenium into weaned lambs. Levels in liver and kidney exceeded 2000 ng/g only during the first day after treatment.
Downloaded by [Michigan State University] at 20:50 02 January 2015
INTRODUCTION
In this country, 5 mg selenium; usually as an aqueous solution of sodium selenate, is administered orally or subcutaneously at intervals to prevent or cure d~ficiency symptoms in the weaned lamb or adult sheep(4). Apart from analyses of tissues from a single animal dosed orally and slaughtered 5 days later3), there is no evidence that selenium residues in meat and other edible tissues do not exceed the maximum level (2000 ng/g) pennitted in foodstuffs in this country(2). Preliminary experiments indicated that subcutaneous dosage produces higher tissue selenium levels than oral dosage, and that the highest levels can be found well within the 8-day period covered by the trial reported here.
kidney cortex exceeded the 2000 nglg limit for human foodstuffs in New Zealand. These undesirably high levels, however, were present for only a few hours after administration of the dose; they did not exceed the limit to any alarming extent and, furthermore, it would be a rare occasion • on which an animal would be dosed with selenium just before slaughter. The results, therefore, indicate that consumption of edible tissue from sheep dosed with 5 mg selenium involves no unacceptable hazard to human health. TABLE: SELENIUM IN TISSUES OF 8-MONTH-OLD LAMBS AFTER SUBCUTANEOUS INJECTION OF SODIUM SELENATE (5 mg Se/25 kg liveweight)
RESULTS AND DISCUSSION
The results in the Table indicate a rapid uptake of selenium from the site of injection. Levels in some samples ofliverand
* Wallace ville Animal Research Centre, Research Division, Ministry of Agriculture and Fisheries, Private Bag, Upper Hutt, New Zealand.
Weight
Time after
No.
kg
injection
Selenium 1•••ls in rresh tissue (ng/g) Blood
Liver
(ng/mI)
I 2 3 4 5 6 7 S 9 10
MATERIALS AND METHODS
Twenty-six Romney wether lambs, aged about 8 months, were reared at Wallace ville where pastures are marginally selenium-deficient(!). Sodium selenate solution was administered to 24 animals by subcutaneous injection so that each received 5 mg selenium/25 kg liveweight. Two animals were left untreated. For the first 3 hours after treatment the sheep were held in the yards. The remaining 13 were then returned to pasture until slaughter. At the times shown in the Table, the animals were slaughtered and samples of blood, liver, kidney cortex, heart muscle and skeletal muscle were taken from each one for selenium analyses. The same tissues were taken from the two controls, one killed at the beginning of the experiment and the other 8 days later when the last of the treated animals was slaughtered. Selenium levels in the samples were determined by Watkinson's method(5), and are expressed as nglg offresh tissue.
Sbeep
II
12 13 14 15 16 17 18 19 20 21 22 23 2~ 25 26
30.0 35.0 30.0 27.5 24.5 29.5 2S.0 29.0 25.0 29.5 30.5 32.0 30.5 29.0 28.5 26.0 36.0 30.0 30.5 31.0 26.0 26.0 27.0 29.5 31.0 35.5
o(Control) o(Control) 5min 8min Ilmin 17min 21 min 26 min 32 min 45 min Ih I h30min 2h 7min 3h Omin 4h 15min 6hOmin Sh30min 12h Omin 17h Omin I day I day 10 h 2day Oh 2day20h 4day Oh 5day 16h 8day Oh
31 31 190 220 190 220 420 490 530 510 590 560 560 590 460
280
240 210 150 140 120 130 120 110 60
29 28 170 170 220 300 560 700 760 1230 1320 1580 1640 1690 1750 2100 1220 2400 1650 780 870 950 1000 1340 520
Kidney rorte.
Heart muscle
740 710 780 750 790
26 39 120 150 110 150 210 240 260 200 240 260 290 290 340 260 270 180 150 105 130 107 95 104 87
600
990
870 1100 1340 1560 2260 2000 1930 2380 1870 2450 1780 1910 1280 1330 1340 1190 1190 700 1130
84
Skelelal
muscl. 13 15 38 42 43 43 80 78 98 108 91 95 86
82 84
60 74 73 58 34 39 32 36 32 30 26
REFERENCES
Andrews, E. D.; Hogan. K. G,; Sheppard, A. D. (1976): Selenium in soils, pastures and animal tissues in relation to the growth of young sheep on a marginally selenium-deficient area. N.Z. ve/.J.Z4: 111-6. (2) Anon. (1973): The Food and Drug Regulations 1973. N.Z. Government Printing Office. Wellington. (3) Hartley, W. J. (1967): Symposium: Selenium in Biomedicine; Chapter 5: Levels of selenium in animaJ tissues and methods of selenium administration. Ed. O. H. Muth. The A. V.I. Publishing Co., Westport, Connecticut, U.S.A. (4) Technical Committee N .Z. Vet. Assn. (1962): Diseases of Domestic Animals in New Zealand, pp. 221-2. Edilorial Services Ltd. Wellin~on. (5) Watkinson, J. H. (1966): Fluorimetric determinatIon of selenium in biological material with 2, 3-diaminonaphthalene. Analyt.Chem.,38: 92-7. (I)
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Selenium residues in sheep meat a
J.B. Stephenson & A.B. Grant
a
a
Research Division, Ministry of Agriculture and Fisheries , Wallaceville Animal Research Centre , Private Bag, Upper Hutt, New Zealand Published online: 23 Feb 2011.
To cite this article: J.B. Stephenson & A.B. Grant (1979) Selenium residues in sheep meat, New Zealand Veterinary Journal, 27:11, 232-232, DOI: 10.1080/00480169.1979.34659 To link to this article: http://dx.doi.org/10.1080/00480169.1979.34659
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
VOL. 27
NEW ZEALAND VETERINARY JOURNAL
232
Selenium residues in sheep meat J. B. Stephenson· and A. B. Grant·
N.Z. vet.J.27: 232 ABSTRACT
Selenium did not exceed lOS ng/g in muscle after single subcutaneous doses of 5 mg selenium into weaned lambs. Levels in liver and kidney exceeded 2000 ng/g only during the first day after treatment.
Downloaded by [Michigan State University] at 20:50 02 January 2015
INTRODUCTION
In this country, 5 mg selenium; usually as an aqueous solution of sodium selenate, is administered orally or subcutaneously at intervals to prevent or cure d~ficiency symptoms in the weaned lamb or adult sheep(4). Apart from analyses of tissues from a single animal dosed orally and slaughtered 5 days later3), there is no evidence that selenium residues in meat and other edible tissues do not exceed the maximum level (2000 ng/g) pennitted in foodstuffs in this country(2). Preliminary experiments indicated that subcutaneous dosage produces higher tissue selenium levels than oral dosage, and that the highest levels can be found well within the 8-day period covered by the trial reported here.
kidney cortex exceeded the 2000 nglg limit for human foodstuffs in New Zealand. These undesirably high levels, however, were present for only a few hours after administration of the dose; they did not exceed the limit to any alarming extent and, furthermore, it would be a rare occasion • on which an animal would be dosed with selenium just before slaughter. The results, therefore, indicate that consumption of edible tissue from sheep dosed with 5 mg selenium involves no unacceptable hazard to human health. TABLE: SELENIUM IN TISSUES OF 8-MONTH-OLD LAMBS AFTER SUBCUTANEOUS INJECTION OF SODIUM SELENATE (5 mg Se/25 kg liveweight)
RESULTS AND DISCUSSION
The results in the Table indicate a rapid uptake of selenium from the site of injection. Levels in some samples ofliverand
* Wallace ville Animal Research Centre, Research Division, Ministry of Agriculture and Fisheries, Private Bag, Upper Hutt, New Zealand.
Weight
Time after
No.
kg
injection
Selenium 1•••ls in rresh tissue (ng/g) Blood
Liver
(ng/mI)
I 2 3 4 5 6 7 S 9 10
MATERIALS AND METHODS
Twenty-six Romney wether lambs, aged about 8 months, were reared at Wallace ville where pastures are marginally selenium-deficient(!). Sodium selenate solution was administered to 24 animals by subcutaneous injection so that each received 5 mg selenium/25 kg liveweight. Two animals were left untreated. For the first 3 hours after treatment the sheep were held in the yards. The remaining 13 were then returned to pasture until slaughter. At the times shown in the Table, the animals were slaughtered and samples of blood, liver, kidney cortex, heart muscle and skeletal muscle were taken from each one for selenium analyses. The same tissues were taken from the two controls, one killed at the beginning of the experiment and the other 8 days later when the last of the treated animals was slaughtered. Selenium levels in the samples were determined by Watkinson's method(5), and are expressed as nglg offresh tissue.
Sbeep
II
12 13 14 15 16 17 18 19 20 21 22 23 2~ 25 26
30.0 35.0 30.0 27.5 24.5 29.5 2S.0 29.0 25.0 29.5 30.5 32.0 30.5 29.0 28.5 26.0 36.0 30.0 30.5 31.0 26.0 26.0 27.0 29.5 31.0 35.5
o(Control) o(Control) 5min 8min Ilmin 17min 21 min 26 min 32 min 45 min Ih I h30min 2h 7min 3h Omin 4h 15min 6hOmin Sh30min 12h Omin 17h Omin I day I day 10 h 2day Oh 2day20h 4day Oh 5day 16h 8day Oh
31 31 190 220 190 220 420 490 530 510 590 560 560 590 460
280
240 210 150 140 120 130 120 110 60
29 28 170 170 220 300 560 700 760 1230 1320 1580 1640 1690 1750 2100 1220 2400 1650 780 870 950 1000 1340 520
Kidney rorte.
Heart muscle
740 710 780 750 790
26 39 120 150 110 150 210 240 260 200 240 260 290 290 340 260 270 180 150 105 130 107 95 104 87
600
990
870 1100 1340 1560 2260 2000 1930 2380 1870 2450 1780 1910 1280 1330 1340 1190 1190 700 1130
84
Skelelal
muscl. 13 15 38 42 43 43 80 78 98 108 91 95 86
82 84
60 74 73 58 34 39 32 36 32 30 26
REFERENCES
Andrews, E. D.; Hogan. K. G,; Sheppard, A. D. (1976): Selenium in soils, pastures and animal tissues in relation to the growth of young sheep on a marginally selenium-deficient area. N.Z. ve/.J.Z4: 111-6. (2) Anon. (1973): The Food and Drug Regulations 1973. N.Z. Government Printing Office. Wellington. (3) Hartley, W. J. (1967): Symposium: Selenium in Biomedicine; Chapter 5: Levels of selenium in animaJ tissues and methods of selenium administration. Ed. O. H. Muth. The A. V.I. Publishing Co., Westport, Connecticut, U.S.A. (4) Technical Committee N .Z. Vet. Assn. (1962): Diseases of Domestic Animals in New Zealand, pp. 221-2. Edilorial Services Ltd. Wellin~on. (5) Watkinson, J. H. (1966): Fluorimetric determinatIon of selenium in biological material with 2, 3-diaminonaphthalene. Analyt.Chem.,38: 92-7. (I)
