Correspondence
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left optic nerve as well (Plate lb). By com- actinomycetales, (family Actinomycetaceae) parison, untreated rabbits did not show gross and the genus has only one species (Bergey's atrophy of the right optic nerve before the Manual of Determinative Bacteriology, 1975). 30th day and was less severe than the treated The organism may be associated with perioanimals by the 90th day (Plate lc). Histo- dontal disease (Lesher, Gerencser & Morrison, logical examination of semi-serial 1 nm sec- 1977; Scharfen, 1975), dental plaque formations of the whole length of the optic nerves tion (Holmberg & Forsum, 1973; Ritz, 1963), and chiasmas on the 5th day revealed that the other diseases of the mouth (Jordan & Sumney lesion did not extend past the chiasma in the 1973; Roth, 1973) and has been isolated from treated animals with very little infiltration of other sites (Brown, George & Waters, 1969; macrophages compared with non-treated Roth & Flanagan, 1969) including the animals. In the 10th and 15th day post- appendix (Scharfen, 1975). It is a normal treatment animals the picture was reversed, inhabitant of the mouth and mainly causes the lesion appeared more active and spongy. infection in compromised hosts (Rother, From these preliminary results it would 1957; Roth & Thurn, 1962). Publications on appear that initially both Ara-A and Ara-C antibiotic sensitivites are infrequent (Roth, treatment of HSV infection in rabbits delayed 1957; Roth & Tbura, 1962). In this paper, the onset of neurological signs and slowed the sensitivities to 18 antibiotics are reported. the progress of the lesion. However, in the A total of 90 strains of R. dentocariosa were long term, treatment has not arrested the isolated from dental plaques, carious lesions, disease, but rather increased the severity of and inflammatory or non-inflammatory periocentral nervous system damage. It is probably dontal tissue in 199 patients. The strains were that some of the deaths occurring in these identified according to the cellular and animals is attributable to drug toxicity. colonial morphology, and biochemical reacWe are grateful to Upjohn Ltd. for supply- tions (Holmberg & Hallander, 1973; Lesher, ing Cytarabine and to Park Davis and Co. for Gerencser & Gerenscer, 1974; George & adenine arabinoside. Brown, 1967). Immunofluorescent techniques H. K. NARANG were also used for further identification using A. A. CODD immune sera obtained from rabbits (Gerencser Public Health Laboratory, & Slack, 1976; Slack, Landfried & Gerencser, Newcastle upon Tyne NE4 6BE, England 1971). References The sensitivity of the strains to 18 antiAdams, D. H. & Cameron, K. R. Derivative of biotics was tested by a serial twofold dilution adenine arabinoside in experimental herpes method in solid Mueller-Hinton medium encephalitis. Lancet li: 760 (1977). with an inoculum of 10* organisms. Cultures Narang, H. K. & Codd, A. A. The pathogenesis were incubated at 37°C for 18 h and results and pathway into the central nervous system were expressed as the minimum inhibitory after intra-ocular infection of herpes simplex type I in rabbits. Neuropathology and Appliedconcentration (MIC). Tests for fi-lactamase were made in some strains (Gots, 1945). Newobiology 4: 137-50 (1970). The sensitivity of R. dentocariosa strains to Narang, H. K. & Codd, A. A. Progression of herpes encephalitis in rabbits following the the penicillins is shown in Figure 1. The most intra-ocular injection of type I virus. Neuro- active agents were ben2ylpenicillin, ampicillin pathology and Applied Neurobiology (1978), inand amoxycillin; cloxacillin and flucloxacillin press. were less active, and carbenicillin the least, Schacfler, H. J., Beauchamp, L., de Miranda, P., although the organisms were still moderately Elion, G. B., Bauer, D. J. & Collins, P. 9-
469
Downloaded from http://jac.oxfordjournals.org/ at University of Illinois at Urbana-Champaign on March 11, 2015
left optic nerve as well (Plate lb). By com- actinomycetales, (family Actinomycetaceae) parison, untreated rabbits did not show gross and the genus has only one species (Bergey's atrophy of the right optic nerve before the Manual of Determinative Bacteriology, 1975). 30th day and was less severe than the treated The organism may be associated with perioanimals by the 90th day (Plate lc). Histo- dontal disease (Lesher, Gerencser & Morrison, logical examination of semi-serial 1 nm sec- 1977; Scharfen, 1975), dental plaque formations of the whole length of the optic nerves tion (Holmberg & Forsum, 1973; Ritz, 1963), and chiasmas on the 5th day revealed that the other diseases of the mouth (Jordan & Sumney lesion did not extend past the chiasma in the 1973; Roth, 1973) and has been isolated from treated animals with very little infiltration of other sites (Brown, George & Waters, 1969; macrophages compared with non-treated Roth & Flanagan, 1969) including the animals. In the 10th and 15th day post- appendix (Scharfen, 1975). It is a normal treatment animals the picture was reversed, inhabitant of the mouth and mainly causes the lesion appeared more active and spongy. infection in compromised hosts (Rother, From these preliminary results it would 1957; Roth & Thurn, 1962). Publications on appear that initially both Ara-A and Ara-C antibiotic sensitivites are infrequent (Roth, treatment of HSV infection in rabbits delayed 1957; Roth & Tbura, 1962). In this paper, the onset of neurological signs and slowed the sensitivities to 18 antibiotics are reported. the progress of the lesion. However, in the A total of 90 strains of R. dentocariosa were long term, treatment has not arrested the isolated from dental plaques, carious lesions, disease, but rather increased the severity of and inflammatory or non-inflammatory periocentral nervous system damage. It is probably dontal tissue in 199 patients. The strains were that some of the deaths occurring in these identified according to the cellular and animals is attributable to drug toxicity. colonial morphology, and biochemical reacWe are grateful to Upjohn Ltd. for supply- tions (Holmberg & Hallander, 1973; Lesher, ing Cytarabine and to Park Davis and Co. for Gerencser & Gerenscer, 1974; George & adenine arabinoside. Brown, 1967). Immunofluorescent techniques H. K. NARANG were also used for further identification using A. A. CODD immune sera obtained from rabbits (Gerencser Public Health Laboratory, & Slack, 1976; Slack, Landfried & Gerencser, Newcastle upon Tyne NE4 6BE, England 1971). References The sensitivity of the strains to 18 antiAdams, D. H. & Cameron, K. R. Derivative of biotics was tested by a serial twofold dilution adenine arabinoside in experimental herpes method in solid Mueller-Hinton medium encephalitis. Lancet li: 760 (1977). with an inoculum of 10* organisms. Cultures Narang, H. K. & Codd, A. A. The pathogenesis were incubated at 37°C for 18 h and results and pathway into the central nervous system were expressed as the minimum inhibitory after intra-ocular infection of herpes simplex type I in rabbits. Neuropathology and Appliedconcentration (MIC). Tests for fi-lactamase were made in some strains (Gots, 1945). Newobiology 4: 137-50 (1970). The sensitivity of R. dentocariosa strains to Narang, H. K. & Codd, A. A. Progression of herpes encephalitis in rabbits following the the penicillins is shown in Figure 1. The most intra-ocular injection of type I virus. Neuro- active agents were ben2ylpenicillin, ampicillin pathology and Applied Neurobiology (1978), inand amoxycillin; cloxacillin and flucloxacillin press. were less active, and carbenicillin the least, Schacfler, H. J., Beauchamp, L., de Miranda, P., although the organisms were still moderately Elion, G. B., Bauer, D. J. & Collins, P. 9-
