Acids Research, Vol. 18, No. 9 2815 k.) © 1990 Press 1990 Oxford University Press ~~~Nucleic Oxford University
Sequence of the dermonecrotic toxin of Pasteurella mnultocida ssp. multocida W.E.C.M.Buys, H.E.Smith, A.M.l.E.Kamps', E.M.Kampl and M.A.Smits Department of Molecular Biology and 'Department of Bacteriology, Central Veterinary Institute, Box 65, 8200 AB Lelystad, The Netherlands
Submitted March 21, 1990
Atrophic
rhinitis is
EMBL accession
major respiratory disease in pigs. Pasteurella produce a dermonecrotic toxin (DNT), are associated with the severe progressive form of the disease. The toxA gene encoding the DNT has recently been isolated (1, 2, 3). Sequence analysis revealed an open reading frame of 3858 nucleotides that could encode a protein of 1286 am-ino acids (146,306 daltons). No significant homologies a
no.
P0
X52478
REFERENCES
multocida ssp. multocida strains that
were
between
found
this
EMBL/GENBANK data
MKXT
K H
F
and
sequence
sequences
in
1.
2. 3.
Petersen,S.K. and Foged,N.T. (1989) Infect. Inunun. 57, 3907-3913. Lax,A.J. and Chanter,N. (1990) J. Gen. Microbiol. 136, 81-87.
Kamps,A.M.I.E., Kamp,E.M.
Len.
and
Smits,M.A. (1990) FEMS Microbiol.
67, 187-190.
the
bank.
F
N
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5
5
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1801
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L D
EBKQG0 LM H B L M EL
1921
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2816 Nucleic Acids Research, Vol. 18, No. 9 H
D
N
V
P
P
F
N
N P
Y
L Y Y K G N I I A A I D R B X F V V F K L Q A wGIATAGaIG MAPAAAATACCAG 6A L D 8 L L N 8 T P T R P M P G L V Q Y L K I P A T V V D B D G A W Q F L P 3241 _ .c S AAT %A L B D V A R V P I B V T L NW Q V L T P P Q K I LTVV G} L X Q F X L T A F P 3361 _AA.__ _ _ _ A1'TAA T AlLTAAAAAkTLIG m kAMSAATAG'TTG7G7TX 3CAAAT R L P L L E N S VB T Q S D L RE E L M Q K I D A I K N D V K M N J L V C M 3481 AAOA ND .fV Liq 0 C D S V B P X V A A R L K D M A L E A M G A 8 I T W W R R M F GO 36011 ACAWCT cm QACCAA,a AT7ACCO B H Q M H T T A F K F A GK V D A F A H L Q v H D Q L D T T I L I L P V 3721 UOTAM AAAACAGAAAC qP c IT D D W A L I A Q R N R A I N P F V E Y V K T G N M L A L F M P P L F T X P R 3841 .M'.AAMMA.AT.A& L T R A L -
3121
A
3961
B
8
Sequence of the dermonecrotic toxin of Pasteurella mnultocida ssp. multocida W.E.C.M.Buys, H.E.Smith, A.M.l.E.Kamps', E.M.Kampl and M.A.Smits Department of Molecular Biology and 'Department of Bacteriology, Central Veterinary Institute, Box 65, 8200 AB Lelystad, The Netherlands
Submitted March 21, 1990
Atrophic
rhinitis is
EMBL accession
major respiratory disease in pigs. Pasteurella produce a dermonecrotic toxin (DNT), are associated with the severe progressive form of the disease. The toxA gene encoding the DNT has recently been isolated (1, 2, 3). Sequence analysis revealed an open reading frame of 3858 nucleotides that could encode a protein of 1286 am-ino acids (146,306 daltons). No significant homologies a
no.
P0
X52478
REFERENCES
multocida ssp. multocida strains that
were
between
found
this
EMBL/GENBANK data
MKXT
K H
F
and
sequence
sequences
in
1.
2. 3.
Petersen,S.K. and Foged,N.T. (1989) Infect. Inunun. 57, 3907-3913. Lax,A.J. and Chanter,N. (1990) J. Gen. Microbiol. 136, 81-87.
Kamps,A.M.I.E., Kamp,E.M.
Len.
and
Smits,M.A. (1990) FEMS Microbiol.
67, 187-190.
the
bank.
F
N
S D
F T VKXGKS
N I
A D
L C
T D
H
EK I I N
EQ0
L E
Y R
V T
F RR
P D
KQ0
L NN
VKXWKx
LKXN
I
121
E VF
FOG0M
I
N R
Y
F
N
IFS
K Y F
D
D
W,D
L
L N
G P N E ACMAAG7
Q
I
Y
H
S
ML
D
T
P
N P
RKX I V
KQO
D
P
E
IFP
V
B
KEBG A Y Y P D H D Y GP E Y N P
V
W
L
Y
241
T
T
SB0
D
SB8 DG N V F H Y NB8 L8B ES
D
A
C L 14
N
361
S L AB
N
0 1
I
L
YB8 V 0
491
601
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B
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F
14
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721
841
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MM
Q
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961
NKXYI
L YN PT H
LEBT
TKRD Y AL S L
L ET
B NL
1081
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B
S E
F
IS
L
I
1201
D M L V P A VG I
PI N F A LBS A T A L 0
L
SBS D IV
V
NOGD
S
YE KXR K Y 0 1
1321
GBS LVQ
S AL
F T
G IN
L I
P V
SSF
BKS
TB
B
B I
T D
B N
L H K
FIR I B P IT F Q
EI
KGB
I S
B
T AB
S I
S
P
I L
DI
P A
F
FT
1441
B B Q A LA Q R F B
I
V B
B
L
K R
LOGG N K
BE4
H
D D P
P K
I
K L V KR
1561
L
N
N
E N Q
P
L
V V
L V
SEBV I N
1681
P VT
NKX T YYVB N AKXL L GG
S VI
GKXP I
G B
S
YKXR I
L AKXLQK
I
Y B
Q
5
5
N
S
P
YBS P F R IG L B GOV WT P EBV LKXAKRA
1801
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H
N SN
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Q
K A L
L D
EBKQG0 LM H B L M EL
1921
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14
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2161
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E MAGKXT S B ST A D L I RKFA L QD T V I S A P F K 0 Y A G tAI P B A I D F P V K Y V I B D I S V F D K IQ T N' Y W
2291
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2816 Nucleic Acids Research, Vol. 18, No. 9 H
D
N
V
P
P
F
N
N P
Y
L Y Y K G N I I A A I D R B X F V V F K L Q A wGIATAGaIG MAPAAAATACCAG 6A L D 8 L L N 8 T P T R P M P G L V Q Y L K I P A T V V D B D G A W Q F L P 3241 _ .c S AAT %A L B D V A R V P I B V T L NW Q V L T P P Q K I LTVV G} L X Q F X L T A F P 3361 _AA.__ _ _ _ A1'TAA T AlLTAAAAAkTLIG m kAMSAATAG'TTG7G7TX 3CAAAT R L P L L E N S VB T Q S D L RE E L M Q K I D A I K N D V K M N J L V C M 3481 AAOA ND .fV Liq 0 C D S V B P X V A A R L K D M A L E A M G A 8 I T W W R R M F GO 36011 ACAWCT cm QACCAA,a AT7ACCO B H Q M H T T A F K F A GK V D A F A H L Q v H D Q L D T T I L I L P V 3721 UOTAM AAAACAGAAAC qP c IT D D W A L I A Q R N R A I N P F V E Y V K T G N M L A L F M P P L F T X P R 3841 .M'.AAMMA.AT.A& L T R A L -
3121
A
3961
B
8
