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OPEN
received: 09 November 2016 accepted: 07 March 2017 Published: 09 May 2017
Serum glycopattern and Maackia amurensis lectin-II binding glycoproteins in autism spectrum disorder Yannan Qin1,*, Yanni Chen2,*, Juan Yang1, Fei Wu1, Lingyu Zhao1, Fuquan Yang3, Peng Xue3, Zhuoyue Shi4, Tusheng Song1 & Chen Huang1 The pathophysiology of autistic spectrum disorder (ASD) is not fully understood and there are no diagnostic or predictive biomarkers. Glycosylation modified as many as 70% of all human proteins can sensitively reflect various pathological changes. However, little is known about the alterations of glycosylation and glycoproteins in ASD. In this study, serum glycopattern and the maackia amurensis lectin-II binding glycoproteins (MBGs) in 65 children with ASD and 65 age-matched typically developing (TD) children were compared by using lectin microarrays and lectin-magnetic particle conjugateassisted LC-MS/MS analyses. Expression of Siaα2-3 Gal/GalNAc was significantly increased in pooled (fold change = 3.33, p
OPEN
received: 09 November 2016 accepted: 07 March 2017 Published: 09 May 2017
Serum glycopattern and Maackia amurensis lectin-II binding glycoproteins in autism spectrum disorder Yannan Qin1,*, Yanni Chen2,*, Juan Yang1, Fei Wu1, Lingyu Zhao1, Fuquan Yang3, Peng Xue3, Zhuoyue Shi4, Tusheng Song1 & Chen Huang1 The pathophysiology of autistic spectrum disorder (ASD) is not fully understood and there are no diagnostic or predictive biomarkers. Glycosylation modified as many as 70% of all human proteins can sensitively reflect various pathological changes. However, little is known about the alterations of glycosylation and glycoproteins in ASD. In this study, serum glycopattern and the maackia amurensis lectin-II binding glycoproteins (MBGs) in 65 children with ASD and 65 age-matched typically developing (TD) children were compared by using lectin microarrays and lectin-magnetic particle conjugateassisted LC-MS/MS analyses. Expression of Siaα2-3 Gal/GalNAc was significantly increased in pooled (fold change = 3.33, p
