INFCTION AND IMUMNITY, Jan. 1977, p. 335436

Vol. 15, No. 1 Printed in U.S.A.

Copyright 0 1977 American Society for Microbiology

Serum Opacity Factor of Staphylococcus epidermidis SAWSAN H. M. EL TAYEB* AiN EZZAT M. M. NASR Clinical Pathology Department, El Azhar University, and Department of Clinical Pathology, College, El Azhar University, Cairo, Egypt

Girls Islamic

Received for publication 4 June 1976

Three Staphylococcus epidermidis strains produced a factor giving rise to opacity in different sera but not in albumin. Serum opacity factor was resistant to age and heat and active in acidic media.

Three strains (laboratory numbers 612NS, when the supernatant was added to human or 618NS, and 725NS) of beta-hemolytic staphylo- bovine albumin. cocci isolated from the skin area behind the ear The recent prestaining technique of Mcof apparently healthy children were coagulase Donald and Bermes (3) for paper electrophorenegative (1) and formed acid from glucose, lactose, sucrose, and late from mannitol. An overnight staphylococcal culture on Todd-Hewitt TABLE 1.

1.6

Analysis of serum and pseudoglobulin

1.6.

before and after adding SOF

1.4

lph

Material

Beta Chylomicronif

29 31 Horse serum 15.5 41.2 Horse serum + SOF 54 23 Pseudoglobulin 63.3 Pseudoglobulin + SOF 13 a Percentage of total lipoprotein (LP).

To

1,2

an

1,0

40 43.3 23 23.3

0O. 0.* 04

0 2

-001o1

i

20 20

60 50 d0

70

i0

.

60 1001;06i0 I~MINU TES

FIG. 2. Effect of heat on SOF activity. Symbols: O-O, heated at 600C; O----O, heated

at 1000C. E

2.

4

1..

9-

1.6

-

0.

.1-

2

16.

1.4 v. 4

Serum opacity factor of Staphylococcus epidermidis.

INFCTION AND IMUMNITY, Jan. 1977, p. 335436 Vol. 15, No. 1 Printed in U.S.A. Copyright 0 1977 American Society for Microbiology Serum Opacity Facto...
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