Shallow water heterobranch sea slugs (Gastropoda: Heterobranchia) from the Regio´n de Atacama, northern Chile Juan Francisco Araya1,2 and A´ngel Valde´s3 1

Departamento de Geologı´a, Universidad de Atacama, Copiapo´, Regio´n de Atacama, Chile Programa de Doctorado en Sistema´tica y Biodiversidad, Universidad de Concepcio´n, Concepcio´n, Chile 3 Department of Biological Sciences, California State Polytechnic University, Pomona, California, United States 2

ABSTRACT The coast of northern Chile has been sparsely studied in regards to its invertebrate fauna, with just a few works reviewing the distribution of local mollusks. This work presents a survey of the shallow water heterobranch sea slugs currently occurring around the port of Caldera (27  S), in the Regio´n de Atacama, northern Chile. Eight species of sea slugs were found in this study: Aplysiopsis cf. brattstroemi (Marcus, 1959), Baptodoris peruviana (d’Orbigny, 1837), Diaulula variolata (d’Orbigny, 1837), Doris fontainii d’Orbigny, 1837, Onchidella marginata (Couthouy in Gould, 1852), Phidiana lottini (Lesson, 1831), Tyrinna delicata (Abraham, 1877) and the new species Berthella schroedli sp. nov., described herein. All of the species found in the area are endemic to South America, having distributions in the southeastern Pacific and South Atlantic Oceans, from Ancash, Peru´ to Peninsula Valde´s, Argentina, and two of them represent species which are endemic to the Chilean coasts (Aplysiopsis cf. brattstroemi and Berthella schroedli). The finding of a previously undescribed species emphasizes the need of further surveys, particularly in subtidal and deeper waters, in order to improve the knowledge on this neglected fauna in Atacama. Submitted 10 November 2015 Accepted 2 April 2016 Published 2 May 2016 Corresponding author Juan Francisco Araya, [email protected] Academic editor Robert Toonen Additional Information and Declarations can be found on page 15 DOI 10.7717/peerj.1963 Copyright 2016 Araya & Valdés Distributed under Creative Commons CC-BY 4.0

Subjects Biodiversity, Conservation Biology, Marine Biology, Taxonomy, Zoology Keywords Eastern Pacific, New taxa, Nudibranchia, Opisthobranchia, Pleurobranchidae,

Sacoglossa, Onchidiidae, Chromodorididae, Dorididae, Shallow water

INTRODUCTION The mollusks of the Regio´n de Atacama, in northern Chile, have been sparsely studied; most of the species commonly present in the area were described in the nineteenth century (Broderip & Sowerby, 1832; Sowerby, 1832; Sowerby, 1833; d’Orbigny, 1834–1847; Gould, 1852; Hupe´, 1854; Gay, 1854, Philippi, 1860, among others), with a few works reviewing species during the past century (Dall, 1909; Gigoux, 1932; Gigoux, 1934; Rehder, 1945) and, more recently, with several works describing new species (Osorio, 2012; Araya, 2013; Araya, 2015a; Araya, 2015b; Miquel & Araya, 2013; Collado, 2015; Araya & Reid, 2016) or giving new records (Araya & Araya, 2015a). Regarding heterobranch sea slugs in particular (sensu Camacho-Garcı´a et al. (2014) and Padula, Wirtz & Schro¨dl (2014)),

How to cite this article Araya and Valde´s (2016), Shallow water heterobranch sea slugs (Gastropoda: Heterobranchia) from the Regio´n de Atacama, northern Chile. PeerJ 4:e1963; DOI 10.7717/peerj.1963

Table 1 Heterobranch sea slugs found in the Region of Atacama, northern Chile; species, distribution, ecology and references. Occurring species involve those cited by Marcus (1959), Schro¨dl (1996a), Schro¨dl (2003), and material examined in this work. Species

Distribution

Ecology 



References

Aplysiopsis cf. brattstroemi (Marcus, 1959)

Antofagasta (23 39′S; 70 25′W) to Bahia de Sea floor, subtidal Coliumo (36 32′S; 72 57′W), Chile

Schro¨dl (1996a)

Baptodoris peruviana (d’Orbigny, 1837)

San Lorenzo (12  S), Peru to Valparaiso, Chile (33 02′S, 71 38′W)

Sea floor, epifaunal, subtidal

Fischer & Cervera (2005a) and Fischer & Cervera (2005b)

Berthella schroedli sp. n.

Caldera (27  S), Chile

Under sunken rocks, infaunal, This work subtidal

Diaulula variolata (d’Orbigny, 1837)

Ica (14  S), Peru´ to Bahı´a de San Vicente (36  S), Chile

Sea floor, epifaunal, subdtidal Fischer & Cervera (2005a), Fischer & Cervera (2005b) and Uribe et al. (2013)

Doris fontainii (d’Orbigny, 1837)

Islote Ferrol (09 08′22″S; 78 37′15″W), Ancash, Peru to northern Argentina

Sea floor, epifaunal, subtidal

Onchidella marginata (Couthoy in Gould, 1852)

Iquique (20  S), Chile to Isla de los Estados Under rocks, epifaunal, (coordinates), Argentina intertidal

Rosenfeld & Aldea (2010)

Phidiana lottini (Lesson, 1831)

Callao (12 02′S), Peru to Comau Fjord (42 15′S; 72 25′12′W), Chile

Sea floor, epifaunal, subtidal

Schro¨dl et al. (2005), Uribe et al. (2013) and Schro¨dl & Hooker (2014)

Tyrinna delicata (Abraham, 1877)

Sea floor, epifaunal, subtidal Isla Blanca (09  S), Ancash, Peru to Peninsula Valde´s, in the Atlantic Magellan Strait

Schro¨dl & Millen (2001) and Uribe et al. (2013)

Uribe et al. (2013) and Valde´s & Muniaı´n (2002)

only the studies by Bergh (1898), Marcus (1959), Schro¨dl (1996a), Schro¨dl (1996b), Schro¨dl (1997), Schro¨dl (2003), Fischer, van de Velde & Roubos (2007) and most recently Labrı´n, Guzma´n & Sielfeld (2015) have included species from northern Chile. However, a few recent papers dealing with the Peruvian fauna, including some species commonly found in Chilean waters (e.g., Millen et al., 1994; Nakamura, 2006; Nakamura, 2007; Martynov & Schro¨dl, 2011; Uribe & Pacheco, 2012; Uribe et al., 2013; Schro¨dl & Hooker, 2014 and others) have also contributed to the knowledge of this group in the southeastern Pacific. The present study provides records of sea slugs found in shallow waters around Caldera (27  S), Regio´n de Atacama, northern Chile. The coast of this area consists of rocky formations with sparse sandy beaches and a comparatively narrow intertidal zone. Rocky platforms, boulder fields and intertidal pools are common; however, some sheltered areas have open sandy beaches, usually exposed to strong surf. All of the species reviewed in this work are endemic to southern South America; with two of them presenting new distributional records in Chile (Table 1). The aim of this preliminary study is to contribute to the knowledge of the molluscan fauna in Chile, particularly from the largely neglected northern coasts.

MATERIALS AND METHODS The material examined was collected in the summers of 2010–2012 in diverse locations near the port of Caldera (27  S), Region of Atacama, northern Chile. All the collecting was made manually in the intertidal areas, mostly on rocky outcrops and tidal pools. The specimens were deposited in the collections of the Museo de Paleontologı´a de Caldera (MPCCL), Caldera, Chile; Museo de Zoologı´a de la Universidad de Concepcio´n (MZUC), Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Concepcio´n, Chile, California State Polytechnic University Invertebrate Collection (CPIC), Pomona, USA, and in the collection of the Natural History Museum of Los Angeles County Museum (LACM), Los Angeles, USA. Field study permits were not required for this study and none of the species studied herein are currently under legal protection. All the collected specimens were preserved in 95% ethanol. Photographs of living animals were taken with a Canon A530 digital camera and a 10 geologic loupe. All sizes given are living measurements, radular features were examined by scanning electron microscopy (SEM). Color plates were composed with basic image programs and colors of the images were not modified. In order to characterize genetically and barcode the new species of Berthella, DNA extraction was performed using a hot ChelexÒ protocol. Approximately 1–3 mg tissue was taken from one animal and cut into fine pieces for extraction, the tissue was rinsed and rehydrated using 1.0 mL TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) for 20 min. A 10% (w/v) ChelexÒ 100 (100–200 mesh, sodium form; Bio-Rad) solution was prepared using TE buffer. After rehydration, the mixture was then centrifuged, 975.00 mL of the supernatant was removed, and 175.00 mL of the ChelexÒ solution was added. Samples were then incubated at 56  C in a water bath for 20 min, heated to 100  C in a heating block for 8 min, and the supernatant was used for PCR. Folmer’s universal COI primers (Folmer et al., 1994) were used to amplify the region of interest for one specimen. The master mix (for each sample) was prepared using 34.75 mL H2O, 5.00 mL PCR Buffer (ExACTGene; Fisher Scientific), 5.00 mL 25 mM MgCl2, 1.00 mL 40 mM dNTPs, 1.00 mL 10 mM primer 1, 1.00 mL primer 2, 0.25 mL 5 mg/mL Taq, and 2.00 mL extracted DNA. Reaction conditions were an initial denaturation for 3 min at 95  C, 39 cycles of 1) denaturation for 45 sec at 94  C, 2) annealing for 45 sec at 45  C, and 3) elongation for 2 min at 72  C, and a final elongation for 10 min at 72  C. PCR products yielding bands of appropriate size (approximately 695 bp) were purified using the Montage PCR Cleanup Kit (Millipore). Cleaned PCR samples were quantified using a NanoDrop 1000 Spectrophotometer (Thermo Scientific). Sequencing was outsourced to Source Bioscience (Santa Fe Springs, CA, USA). The sequence was assembled and edited using Geneious Pro 8.1.7 (Kearse et al., 2012). Geneious was also used to extract the consensus sequence, which was 658 bp long and is deposited in GenBank (GenBank Voucher Number KU551261). The electronic version of this article in Portable Document Format (PDF) will represent a published work according to the International Commission on Zoological Nomenclature (ICZN), and hence the new names contained in the electronic version are effectively published under that Code from the electronic edition alone. This published work and the nomenclatural acts it contains have been registered in ZooBank, the online registration system for the ICZN. The ZooBank LSIDs (Life Science Identifiers) can be resolved and the associated information viewed through any standard web browser by appending the LSID to the prefix http://zoobank.org/. The LSID for this publication is: urn:lsid:zoobank.org:pub:088D994A-9E1E-4324-A6DF-FCCC2B0E3437. The online version of this work is archived and available from the following digital repositories: PeerJ, PubMed Central and CLOCKSS. Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Figure 1 Species of heterobranch sea slugs found near Caldera, Atacama region, northern Chile (all specimens photographed in situ). (A) Phidiana lottini (Lesson, 1831), Calderilla Beach, inside a valve of Argopecten purpuratus (Lamarck, 1819), L = 23 mm; (B) Tyrinna delicata (Abraham, 1877), Obispito Bay, L = 10 mm; (C) Baptodoris peruviana (d’Orbigny, 1837), Ramada Beach, L = 23 mm; (D) Diaulula variolata (d’Orbigny, 1837), El Pulpo Beach, L = 34 mm.

RESULTS Systematics Heterobranchia Order Nudibranchia Cuvier, 1817 Superfamily Aeolidioidea Gray, 1827 Family Facelinidae Bergh, 1889 Genus Phidiana Gray, 1850 Type species Eolidia patagonica d’Orbigny, 1836, by subsequent designation by Alder & Hancock (1855). Phidiana lottini (Lesson, 1831) (Fig. 1A) Eolidia lottini Lesson, 1831: 290, pl. 14, fig. 6. Cavolina lottini d’Orbigny, 1837: 194. Phidiana inca Gray, 1850: 108; Bergh, 1867: 100, pl. 3, figs. 1–13; Marcus, 1959: 79, ´ lamo & Valdivieso, 1997: 85. Phidiana lottini Schro¨dl, 1996a: 41, pl. II, figs. 184–190; A Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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fig. 13. pl. VII, fig. 41; Schro¨dl, 2003: 83, figs. 51, 63, 64, 88; Schro¨dl, 2009: 539; Schro¨dl et al., 2005: 7, pl. 2, fig. 17; Uribe et al., 2013: 52, fig. 3J; Schro¨dl & Hooker, 2014: 54, figs. 12, 13; Uribe et al., 2014: 167. A detailed chresonymy can be found in Schro¨dl (2003). Material examined: Two specimens collected in a tidal pool in rocky outcrops, Playa Brava (27 03′S; 70 49′W), Caldera, Regio´n de Atacama, Chile (MZUC 39608); and one specimen collected inside an empty Austromegabalanus psittacus shell in Calderilla (27 05′S; 70 50′W), Caldera, Regio´n de Atacama, Chile (MPCCL 90216A). Diagnosis: Elongate body of silky white to sometimes reddish color, covered by 20–26 parallel rows of conspicuously colored cerata. Dorsum with a fine longitudinal white line. Cerata with bands of brown and orange at base and with bright whitish tips. Rhinophores annulate, yellowish white. Oral tentacles long and pinkish-white. Anterior foot corners slightly extended. Distribution: Phidiana lottini has been recorded in Chile from Punta Blanca, Arica (18 29′S; 70 20′W) to the Guaitecas Islands (44  S), southern Chile (Schro¨dl, 2003; Schro¨dl & Hooker, 2014). This species has also been recorded from Ancash, Isla Santa, Lima, and Callao (12 02′S), central Peru (Uribe et al., 2013; Schro¨dl & Hooker, 2014). Remarks: Phidiana lottini is easily recognizable from other aeolid sea slugs found in northern Chile because of the cerata arranged in parallel rows and the presence of a white dorsal line between the rhinophores. This is a comparatively common nudibranch in the area, usually found in protected localities. Egg masses of this species are loosely coiled whitish spiral ribbons, of about 30 mm in diameter (see Schro¨dl, 2003).

Superfamily Doridoidea Family Chromodorididae Bergh, 1891 Genus Tyrinna Bergh, 1898 Type species Tyrinna nobilis Bergh, 1898 (= Tyrinna delicata (Abraham, 1877)), by monotypy. Tyrinna delicata (Abraham, 1877) (Fig. 1B) Doris delicata Abraham, 1877: 211, pl. XXX, figs. 20–22. Tyrinna nobilis Bergh, 1898: 523, pl. 30, figs. 21–29, pl. 32, figs. 21–24; Marcus, 1959: 31, figs. 45–53; Muniaı´n, Valde´s & Ortea, 1996: 265, figs. 2–6; Schro¨dl, 1996a: 22, pl. 3, fig. 15; Schro¨dl, 1997: 41; Schro¨dl, 2003: 31, figs. 15, 70; Schro¨dl et al., 2005: 4, pl. 1, fig. 8; Schro¨dl & Millen, 2001: 1146, figs. 1–6; Schro¨dl, 2009: 521; Aldea, Ce´sped & Rosenfeld, 2011: 43, fig. 3C. Uribe et al., 2013: 48, fig. 2A. Tyrinna pusae Marcus, 1959: 33, figs. 54–64. A detailed chresonymy can be found in Schro¨dl (2003). Material examined: One specimen collected under rocks at low tide, in tidal pools in rocky outcrops, South of Obispito (26 45′51″S; 70 45′07″W), Caldera, Regio´n de Atacama, Chile (MPCCL 90216B). Diagnosis: Body oval-elongate, translucent-whitish, with opaque white lines surrounding the edges of foot and mantle. Dorsum smooth, with irregular and submarginal rows of orange spots, absent from the central region of mantle. Oral tentacles longitudinally enrolled. Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Anterior part of foot bilabiate, forming a thick lip. Posterior end of the foot extending beyond the mantle in crawling individuals (see Uribe et al. (2013) for a more complete description). Distribution: From Isla Blanca (09  S), Ancash, Peru to Peninsula Valde´s, in the Atlantic Magellan Strait (Schro¨dl & Millen, 2001; Uribe et al., 2013). This species has been also recorded in the Juan Ferna´ndez Islands, off central Chile. Remarks: Tyrinna delicata is clearly distinguishable from other nudibranchs in northern Chile by the submarginal dorsal rows of orange spots, which are very visible in the translucent whitish mantle. This species, having a complex synonymy, was listed as Tyrinna nobilis until recent, however the discovery of the holotype of Tyrinna delicata (Abraham, 1877) by Schro¨dl & Millen (2001) gave priority to the latter name. Family Discodorididae Bergh, 1891 Genus Baptodoris Bergh, 1884 Type species Baptodoris cinnabarina Bergh, 1884, by monotypy. Baptodoris peruviana (d’Orbigny, 1837) (Fig. 1C) Doris peruviana d’Orbigny, 1837: 188, pl. XV, figs. 7–9. Doriopsis peruviana Dall, 1909: 203. Platydoris punctatella Bergh, 1898: 521, figs. 12–20; Dall, 1909: 203; Schro¨dl, 1996a: 23, ´ lamo & Valdivieso, 1997: 85. Platydoris peruviana pl. IV, fig. 27. Dendrodoris peruviana A Schro¨dl, 2003: 34, figs. 17, 54, 71. Baptodoris peruviana Fischer & Cervera, 2005a: 515, figs. 1–8. Uribe et al., 2013: 51, fig. 3D. Baptodoris? peruviana Schro¨dl & Hooker, 2014: 48, fig. 4. Material examined: One specimen collected under rocks at very low tide, Playa Ramada (27 00′S; 70 48′W) Caldera, Regio´n de Atacama, Chile (MZUC 39607). Diagnosis: Elevated, oval and slightly convex white-yellowish body, with minute brown spots over the notum which is densely covered by very small rounded caryophyllidia. Rhinophores and gills hyaline white, not elevated. Rhinophores are perfoliate with 7–10 lamellae. The branchial tuft consists of 6 uni-bipinnate gills, which form a circle around the anus at the posterior end of the body. Ventrally, the head is small with short digitiform oral tentacles. The foot is narrow, with the anterior edge notched at the mid-line and grooved. The notal margin is white and wider than the foot (see Fischer & Cervera (2005a) for a complete description). Distribution: According to Fischer & Cervera (2005a), this species has been recorded from South of San Lorenzo Island, Lima, Peru to Valparaiso, (33 02′S; 71 38′W) Chile. Genus Diaulula Bergh, 1884 Type species Doris sandiegensis (Cooper, 1863), by monotypy. Diaulula variolata (d’Orbigny, 1837) (Fig. 1D) Doris variolata d’Orbigny, 1837: 186, pl. 16, figs. 1–3. Anisodoris marmorata Marcus, 1959: 45, figs. 98–103; Schro¨dl, 2003: 41, figs. 21, 57, 75; Fischer & Cervera, 2005b: 174. Uribe et al., 2013: 48, fig. 2B. Anisodoris marmorata Bergh, 1898: 515, pl. 30, figs. 5–7 (non Archidoris marmorata Bergh, 1881); Marcus, 1959: 45, figs. 98–103. Anisodoris rudberghi Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Marcus & Marcus, 1967: 69; Schro¨dl, 1996a: 25, pl. IV, figs. 21–22; Schro¨dl, 1996b. Peltodoris marmorata Valde´s & Muniaı´n, 2002: 349, figs. 1D, 4, 5. A detailed chresonymy can be found in Schro¨dl (2003: 39). Material examined: One specimen collected under rocks at very low tide, North of Obispito (26 45′S; 70 45′W), 40 km N of Caldera, Regio´n de Atacama, Chile (MZUC 39606). Diagnosis: Whitish-yellowish body with minute black spots over the notum, which is densely covered by small and narrow caryophyllidia. Wide free mantle rim. Rhinophoral and branchial sheaths elevated, covered with caryophyllidia. Six to seven gills, ramified up to four-five times. Oral tentacles long and digitiform. Foot bilabiate, with upper lip notched. Lip cuticle smooth. Rhinophores have more than 15 lamellae (see Schro¨dl (2003) for a complete description). Distribution: This species has been recorded in Chile from Arica (18  S) to the Bahı´a de San Vicente (36  S), and most recently from Ica, Peru´ (Uribe et al., 2013). Family Dorididae Rafinesque, 1815 Genus Doris Linnaeus, 1758 Type species Doris verrucosa Linnaeus, 1758, by monotypy. Doris fontainii d’Orbigny, 1837 (Fig. 2A) Doris fontainii d’Orbigny, 1837: 189, pl. 15, figs. 1–3. Anisodoris fontaini Odhner, 1926: 85, figs. 70–72, pl. 3, figs. 47–49; Schro¨dl, 1996a: 24, pl. III, fig. 19; Schro¨dl, 2000b: 73, fig. 2–3. Doris fontainei Gay, 1854: 76; Valde´s & Muniaı´n, 2002: 346, figs. 1A–B, 2A–C, 3A–B; Uribe et al., 2013: 51, fig. 3E; Schro¨dl & Hooker, 2014: 47, fig. 2. Archidoris fontaini Schro¨dl, 2003: 45, figs. 24, 58, 76; Schro¨dl, 2009; Schro¨dl et al., 2005: 4, pl. 2, fig. 9; Schro¨dl & Grau, 2006: 5, fig. 2A–B. Material examined: One specimen collected in a tidal pool at Playa El Jefe (27 03′46″S;  70 49′W), Caldera, Regio´n de Atacama, Chile (MZUC 37642). Diagnosis: Orange to brownish body coloration, with a highly arched and large body (up to 10 cm according to Schro¨dl & Hooker (2014)). Notum covered with many small (up to 5 mm in diameter) rounded tubercles. Five to seven tri- to quadripinnate gills. Gills and rhinophores surrounded by elevated sheaths with small tubercles. Oral tentacles triangular, grooved. Foot broad, anteriorly bilabiate and notched. Lip cuticle smooth (see Schro¨dl (2003) for a complete description). Distribution: This species has been recorded from Ancash, Islote Ferrol, Peru (Uribe et al., 2013) to northern Argentina (Valde´s & Muniaı´n, 2002). Remarks: This species is easily recognizable due to its large size, brilliant orange body color and a mantle covered with conspicuous rounded tubercles. Of the examined specimens, none had the dark brown pigment between the tubercles, which Schro¨dl et al. (2005), regarded as characteristic of central and northern Chilean specimens. This was the most common species in the area; they are usually found in the subtidal zone but specimens were also collected from tidal pools at low tide. According to some commercial divers this species is common below 3 m depth near Bahı´a Inglesa (27 07′S; 70 52′W), south of Caldera. Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Figure 2 Species of heterobranch sea slugs found near Caldera, Atacama region, northern Chile (all specimens photographed in situ). (A) Doris fontainii d’Orbigny, 1837, Playa El Jefe, L = 54 mm; (B) Aplysiopsis cf. brattstroemi (Marcus, 1959), Brava Beach, specimen found among filamentous algae in tidal pool, L about 4 mm; (C) Onchidella marginata (Couthouy in Gould, 1852), Playa El Pulpo, L = 12 mm (largest specimen).

Order Pleurobranchomorpha Schmekel, 1985 Superfamily Pleurobranchoidea Gray, 1827 Family Pleurobranchidae Gray, 1827 Genus Berthella Blainville, 1824 Type species Bulla plumula Montagu, 1803, by original designation. Berthella schroedli sp. nov. urn:lsid:zoobank.org:act:9F1D698F-96FB-40B0-A972-3C1F6F15014C (Figs. 3A–3C, 4A–4D, 5A, 5B and 6C). Type material: Holotype MPCCL 90216C, paratypes: LACM 3327 (4 specimens), MPCCL 90216D (4 specimens); other material: CPIC 000827 (5 specimens). All the type material collected at the type locality and preserved in ethanol 96%. Type locality: Playa El Pulpo (27 01′22″S; 70 48′30″W), Comuna de Caldera, Regio´n de Atacama, Chile, intertidal under sunken rocks in rocky coast, 1 m depth, 29 December 2012, coll. & leg. JF Araya. Diagnosis: Intertidal Berthella species with a dark brown-reddish shell decorated with pale radial lines; visible through the translucent yellowish mantle, with an oval and slightly crenulated margin and very small tubercles covering the notum. Description: Body reaching lengths up to 25 mm in fully extended living specimens (Figs. 3A, 3B and 6C). Body uniformly pale yellowish, translucent; with an internal shell of brownish-reddish color, visible through the mantle. Mantle with a smooth appearance, but with very small tubercles covering the dorsum which gives the animal, at high magnification, a somewhat wrinkled appearance. The mantle processes do not show obvious spicules. Dark and minute eyes located behind the base of the rhinophores, hidden beneath the anterior edge of the mantle (Fig. 3B). Notum wide, oval and slightly crenulated, with a broad free margin around. Gill and foot covered by the notum in living specimens, and oral veil and rhinophores partially covered in their posterior part. Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Figure 3 Berthella schroedeli sp. nov. (A) Specimens photographed in situ, under rocks at low tide, Aguas Verdes; (B) Detail of specimen showing the eyes; (C) SEM image of shell (LACM 3327).

Mantle lacking an anterior notch. Rhinophores short and stout, joined together at the base. Foot bilabiate anteriorly. Oral veil trapezoidal, protruding from the mantle. Gill located on the right side of the body, lying longitudinally between the mantle and the foot; it is attached to the body for more than half of its length. Gill bipinnate, with 13 pinnae on either side of the rachis. Rachis smooth, lacking tubercles. Anus located dorsal to the central area of the gill. Egg masses are small white spiral ribbons, up to about 25 mm in diameter (Fig. 6C). Shell: Shell fully internal, flattened, rectangular/oval in shape, elongate and located centrally in the dorsal area, where it covers completely the viscera. Shell reddish brown in color, somewhat nacreous/iridescent, with radial rays of pale yellowish which are visible through the mantle in living specimens. Margins of shell sharp and fragile. Protoconch of about 300 mm in diameter, smooth under low magnification. Teleoconch with fine Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Figure 4 Berthella schroedeli sp. nov., SEM images (LACM 3327). (A) Radular teeth, central portion of the radula; (B) Outermost radular teeth; (C) Lateral teeth, middle portion of the half row; (D) Detail of the jaw platelets.

concentric ridges crossed by very fine radial striae, the first whorls have a cancellated sculpture (Fig. 3C). Radula: Radular formula: 50–53  45–56.0.45–56. Radular teeth hook-shaped lacking denticles (Fig. 4A). Innermost lateral teeth slightly smaller than those from the middle portion of the half row (Fig. 4B). Outermost lateral teeth with a much more elongate cusp than the mid laterals (Fig. 4C). Jaws with elongate cruciform elements rather slender, elongate and lanceolate with a narrower base; each element consisting of a central cusp flanked by 2–3 denticles on either side of a prominent central cusp (Fig. 4D). Reproductive system: The ampulla is long and muscular, merging proximally into the female gland complex. The penis is wide, with an elongate tip; it connects proximally into a short deferent duct that splits into the prostate and the elongate, muscular penial gland. The prostate is convoluted and connects proximally to the female gland complex. A small, unidentified glandular structure connects distally

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Figure 5 Reproductive anatomy of Berthella schroedli sp. nov. (A) Dorsal view of the reproductive system; (B) Detail of some organs covered by the prostate and penial gland. Abbreviations used are: am, ampulla; bc, bursa copulatrix; fgc, female gland complex; pe, penis; pg, penial gland; pr, prostate; sr, seminal receptacle; vg, vagina.

into the prostate and is here referred to provisionally as prostatic gland (prg? in Fig. 5A). The vagina is elongate, straight; it narrows and connects to the round and large bursa copulatrix. The seminal receptacle is elongate, muscular and about twice as long as the bursa copulatrix; it connects to the vagina before it enters the bursa copulatrix. A uterine duct could not be observed (Fig. 5). Habitat: This species is found exclusively under rocks sunken at low tide in an almost infaunal habitat; it can be found associated to encrusting sponges, bryozoans, encrusting algae and to communities of micromollusks including Acar pusilla (Sowerby, 1833), Brachidontes granulata (Hanley, 1843), Liotia cancellata Gray, 1848 and Mitrella unifasciata (Sowerby, 1832). Distribution: This species is somewhat rare but broadly distributed in the area of study; small populations were found only in four localities, in about 40 km of coast, always under rocks. According to Schro¨dl (2003) this genus has records in southern, South America from the southernmost Patagonian shelf (Burdwood Bank), southeastern Atlantic Ocean to southern Chile and north to Quiriquina Island, central Chile. The genus thus extends its distribution in Chile more than 1,100 km to the north.

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Figure 6 Chilean Berthella species. (A) and (B) specimens of Berthella platei (Bergh, 1898) photographed in situ, Caleta de Arena, 20 m depth and Valdivia respectively (photos (A) and (B) courtesy of Dirk Schories); (C) Berthella schroedli sp. nov., specimen sitting on egg masses, Obispito, Caldera.

Etymology: Named in honor of Michael Schro¨dl (Zoologische Staatssammlung Mu¨nchen, Munich, Germany), for his extensive contributions to the Chilean opisthobranchs. Remarks: Of the 16 valid species of Berthella known worldwide (Hermosillo & Valde´s, 2008), only two have been reported for southern South America: Berthella patagonica (d’Orbigny, 1837) and Berthella platei (Bergh, 1898). The western Atlantic Berthella patagonica, distributed from Central Argentina to Peninsula Valde´s, southern Argentina (Schro¨dl, 2003), differs from the new species in having smaller body dimensions, with a very narrow free mantle rim and a notum apparently lacking a porous texture and not covering completely the foot which, in contrast to the new species, has a quadrangular outline (Schro¨dl, 1999; Schro¨dl, 2003). The Magellanic Berthella platei, distributed from the Burdwood Bank, southeastern Atlantic Ocean to Quiriquina Island, Central Chile (Schro¨dl, 1999), differs from the new species in having a more translucent body, of uniform pale pink to pale orange or whitish coloration of living animals (Figs. 6A and 6B), a higher number (15–24) of branchial lamellae versus 11–14 in B. schroedli sp. n. and a paler internal shell, translucent brown to greyish in color, in contrast to the characteristic reddish-brown shell with faint whitish axial streaks of the new species. The radular formula and the elements of the jaws also differ; Berthella schroedli sp. n. have fewer radular rows and less teeth per half row than B. platei, and it has also larger elongate and lanceolate elements with a narrower base and thin denticles, while B. platei have smaller and more triangular elements with a broader base and slightly broader denticles (see Schro¨dl, 1999). The shell length in relation to the body size in B. schroedli is also comparatively larger than in B. platei. In regard to their habitat; the new species has been found almost solely under sunken rocks in relatively shallow water in the intertidal; while Berthella platei is found only subtidally, living in the ocean floor usually under 5 m depth (Dirk Schories, 2013, personal communication). A BLAST-n of the COI sequence of B. schroedli sp. n. returned that the most similar sequence belongs to Berthella

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plumula (AY345025) and is only 84% identical. The sequence of B. schroedli sp. n. is only 83% identical to a sequence of Berthella platei (FJ917492), providing additional evidence that this species is distinct. Other Eastern Pacific species of Berthella include Berthella agassizi (MacFarland, 1909); Berthella californica (Dall, 1900); Berthella grovesi Hermosillo & Valde´s, 2008; Berthella martensi (Pilsbry, 1896); Berthella stellata (Risso, 1826) and Berthella strongi (MacFarland, 1966). All these species differ from Berthella schroedli sp. n. in their subtidal rather than intertidal habitat, and also chiefly in their external coloration, by having opaque white spots (B. agassizii, B. strongi) or light brown spots and/or an orange body with dark brown lines and spots (B. martensi), a marginal notal band (B. californica), dark spots in the middle of thick opaque white ringlets (B. grovesi) or a dorsal streak of white running perpendicularly across the notum, which is translucent white or honey colored (B. stellata). Order Sacoglossa Ihering, 1876 Superfamily Limapontioidea Gray, 1847 Family Hermaeidae Adams & Adams, 1854 Genus Aplysiopsis Deshayes, 1853 Type species Aplysiopsis elegans Deshayes, 1853, by monotypy. Aplysiopsis cf. brattstroemi (Marcus, 1959) (Fig. 2B) Hermaeina brattstroo¨mi Marcus, 1959: 21, figs. 21–27. Aplysiopsis brattstroemi Schro¨dl, 1996a: 45, pl. VIII, fig. 52; Fischer & Cervera, 2005a: 167; Jensen, 2007: 279. Material examined: One specimen photographed alive (not collected); on filamentous algae in tidal pool at very low tide, Playa Brava (27 03′S; 70 49′W), Caldera, Regio´n de Atacama, Chile. Diagnosis: Body minute, up to about 5 mm in examined specimen, with an elongated body, narrowed anteriorly; of brown to deep greenish-black color, with two clear areas at the sides of the head. Several rows of flat longitudinal cerata in the border of the mantle. Enrolled rhinophores. Size up to about 3 cm (see Marcus (1959) for a complete description). Distribution: Aplysiopsis brattstro¨mi has a discontinuous distribution from Antofagasta (23 39′S; 70 25’W), to Bahı´a de Coliumo (36 32′S; 72 57′W) in Chile (Schro¨dl, 1996a). The definite allocation of this specimen is currently not possible as, unfortunately, it was not collected. Order Systellommatophora Pilsbry, 1948 Superfamily Onchidioidea Rafinesque, 1815 Family Onchidiidae Rafinesque, 1815 Genus Onchidella Gray, 1850 Type species Onchidium nigricans Quoy & Gaimard, 1832, by subsequent designation by Fischer and Crosse (1878). Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Onchidella marginata (Couthouy in Gould, 1852) (Fig. 2C) Peronia marginata Couthouy in Gould, 1852: 292; Atlas, 1856: pl. 22, figs. 386a–c. Onchidium chilense Gay, 1854: 120. Onchidella marginata Marcus, 1959: 16, figs. 17–20. Dayrat, 2009: 13. Rosenfeld & Aldea, 2010: 35, figs. 1A–B. A more complete synonymy can be found in Dayrat (2009). Material examined: Ten specimens collected under small rock slabs at low tide, Playa El Pulpo (27 03′S; 70 49′W), Caldera, Regio´n de Atacama, Chile (MZUC 280316). Diagnosis: Body elongate ovate, narrowed anteriorly; back very convex, deep greenishblack, very thickly covered with minute tubercles; margin ornamented with alternate bars of black and white; head broad, bilobed in font, and projecting considerably beyond the mantle when the animal is in motion, of a pale yellow color, tinted bluish about the mouth; tentacles rather long, and bulbous at the extremity, pale slate-color, except at the tips, which are back; under side of the mantle pale yellowish, becoming greenish at the margin, where it shows alternate bands of green and pale yellow (see Gould (1852) for a complete description). Distribution: Onchidella marginata has a discontinuous distribution from Iquique (20  S) to the Magallanes Strait (55  S) in Chile, and to the Isla de los Estados in the South Atlantic of Argentina (Rosenfeld & Aldea, 2010). Remarks: This is the only pulmonate sea slug found in Chile (Valdovinos, 1999; Dayrat, 2009); it is usually found in small communities living under rocks and camouflaging against their surroundings. In the area under study this species share its habitat with other mollusks as the limpet Lottia orbignyi (Dall, 1909), and the chitons Chaetopleura peruviana (Lamarck, 1819) and Radsia barnesi (Gray, 1828).

DISCUSSION The present work updates the knowledge on the scarcely known marine fauna of northern Chile (in particular from the Regio´n de Atacama); from the 65 species of sea slugs (only including Nudibranchia and Pleurobranchoidea) recorded to live in Chilean waters (Schro¨dl, 2003), eight species were recorded in the Regio´n de Atacama, accounting for about 12% of the Chilean sea slug fauna. All of the species occurring in the area have widespread ranges in the southeastern Pacific Ocean, from Ancash, Peru to the Strait of Magellan, in southern Chile and in the South Atlantic Ocean, to Peninsula Valde´s, in Argentina (Table 1). With the exception of Berthella schroedli sp. n., all of the species found in the Regio´n de Atacama also occur in central and southern Chile. The absence of species previously cited for the area (Schro¨dl, 1996a; Schro¨dl, 2003; Schro¨dl & Hooker, 2014), for example Corambe lucea Marcus, 1959; Janolus rebeccae Schro¨dl, 1996a; Schro¨dl, 1996b; Okenia luna Millen et al., 1994 and Thecacera darwini Pruvot-Fol, 1950, among others, could be explained due to the limit of sampling depth, which was restricted to the lower intertidal areas with a maximum of 2 m depth. Heterobranch sea slugs have been rarely treated in studies reviewing the biodiversity of mollusks from northern Chile (e.g. Marincovich, 1973; Guzma´n, Saa´ & Ortlieb, 1998), despite the comparatively high number of species recorded in the country. This is in part explained by the current lack of experts working actively in the field and the Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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difficulties involved in collecting and preserving marine slugs. The finding of a new species of Berthella in northern Chile also highlights the need of further studies in the area or in northern Chile in general, which have recently revealed new invertebrate species (Reiswig & Araya, 2014; Collado, 2015) or new distributions for obscure or rare species, both from shallow and deeper waters (e.g. Araya & Aliaga, 2015; Araya & Araya, 2015b; Araya, Aliaga & Araya, 2015; Araya, 2015c; Fischer, van der Velde & Roubos, 2007; Labrı´n, Guzma´n & Sielfeld, 2015).

ACKNOWLEDGEMENTS We are very grateful to Marta Araya (Caldera, Chile) for her assistance in field collecting, to Carlo Magenta Cunha (Academy of Natural Sciences of Drexel University, Philadelphia, USA), and to Cecilia Osorio (Universidad de Chile, Santiago, Chile) for their help with essential bibliography, to Dirk Schories (University of Rostock, Rostock, Germany) for his help with the images and information on Berthella platei from southern Chile and to Michael Schro¨dl (Zoologische Staatssammlung Mu¨nchen, Germany) and two anonymous reviewers for their helpful corrections and suggestions on the manuscript.

ADDITIONAL INFORMATION AND DECLARATIONS Funding The authors received no funding for this work.

Competing Interests The authors declare that they have no competing interests.

Author Contributions  Juan Francisco Araya conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.  A´ngel Valde´s conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition The following information was supplied regarding data availability: The research in this article did not generate nor collect any raw data/code.

New Species Registration The following information was supplied regarding the registration of a newly described species: Publication LSID: urn:lsid:zoobank.org:pub:088D994A-9E1E-4324-A6DFFCCC2B0E3437. Berthella schroedli sp. nov. LSID: urn:lsid:zoobank.org:act:9F1D698F-96FB-40B0A972-3C1F6F15014C. Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

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Supplemental Information Supplemental information for this article can be found online at http://dx.doi.org/ 10.7717/peerj.1963#supplemental-information.

REFERENCES Abraham PS. 1877. Revision of the anthobranchiate nudibranchiate Mollusca, with descriptions or notices of forty-one hitherto undescribed species. Proceedings of the Zoological Society of London 1877:196–269. ´Alamo V, Valdivieso V. 1997. Lista sistema´tica de moluscos marinos del Peru. Segunda edicio´n, revisada y actualizada. Callao: Publicacio´n Especial Instituto del Mar del Peru´, 183 pp. Aldea C, Ce´sped T, Rosenfeld S. 2011. Opisthobranchs from Bernardo O’Higgins National Park (S. Chile). Thalassas 27(2):37–48. Araya JF. 2013. A new species of Aeneator Finlay, 1926 (Mollusca, Gastropoda, Buccinidae) from northern Chile, with comments on the genus and a key to the Chilean species. ZooKeys 257:89–101 DOI 10.3897/zookeys.257.4446. Araya JF. 2015a. Current status of the non-indigenous molluscs of Chile, with the first record of Otala punctata (Mu¨ller, 1774) (Gastropoda: Helicidae) in the country and new records for Cornu aspersum (Mu¨ller, 1774) and Deroceras laeve (Mu¨ller, 1774). Journal of Natural History 49(29–30):1731–1761 DOI 10.1080/00222933.2015.1006703. Araya JF. 2015b. The Bulimulidae (Mollusca: Pulmonata) from the Regio´n de Atacama, northern Chile. PeerJ 3:e1383 DOI 10.7717/peerj.1383. Araya JF. 2015c. New records of deep-sea sea spiders (Chelicerata: Pycnogonyda) in the southeastern Pacific. Marine Biodiversity 1–5 DOI 10.1007/s12526-015-0416-7. Araya JF, Aliaga JA. 2015. The northernmost record of Tytthosoceros inca Baeza, Ve´liz, Pardo, Lohrmann and Guisado 1997 (Plathyhelminthes: Pseudocerotidae) in Chile. Marine Biodiversity 1–2 DOI 10.1007/s12526-015-0395-8. Araya JF, Aliaga JA, Araya ME. 2015. On the distribution of Physalia physalis (Hydrozoa: Physaliidae) in Chile. Marine Biodiversity 1–5 DOI 10.1007/s12526-015-0417-6. Araya JF, Araya ME. 2015a. The shallow-water chitons (Mollusca, Polyplacophora) of Caldera, Region of Atacama, northern Chile. Zoosystematics and Evolution 91(1):45–58 DOI 10.3897/zse.91.8536. Araya JF, Araya ME. 2015b. The southernmost record of the amphipod Hyperia curticephala (Crustacea: Amphipoda: Hyperiidae) in the Pacific Ocean. Marine Biodiversity Records 8:e40 DOI 10.1017/S1755267215000184. Araya JF, Catala´n R. 2014. A review of the non-bulimulid terrestrial Mollusca from the Region of Atacama, northern Chile. ZooKeys 398:33–51 DOI 10.3897/zookeys.398.4282. Araya JF, Reid DG. 2016. A new species of Echinolittorina Habe, 1956 (Gastropoda: Littorinidae), from a Quaternary shallow water molluscan assemblage in northern Chile. Palaeontologia Electronica 19.1.8A:1–8. Bergh LSR. 1867. Phidiana lynceus og Ismaila monstrosa. Videnskabelige Meddeleser fra Danske Naturhistorisk Forening 97–130. Bergh LSR. 1898. Die Opisthobranchier der Sammlung Plate. Zoologische Jahrbu¨cher, Supplement 4(3):481–582. Broderip WJ, Sowerby GB. 1832. Description of new ( : : : ) Mollusca and Conchifera ( : : : ) part of the collection made by Mr. H. Cuming. Proceedings of the Zoological Society of London 1832:25–33.

Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

16/20

Camacho-Garcı´a Y, Pola M, Carmona L, Padula V, Villani G, Cervera L. 2014. Diversity and distribution of the heterobranch sea slug fauna on the Caribbean of Costa Rica. Cahiers de Biologie Marine 55(1):109–127. Collado GA. 2015. A new freshwater snail (Caenogastropoda: Cochliopidae) from the Atacama Desert, northern Chile. Zootaxa 3925(3):445–449 DOI 10.11646/zootaxa.3925.3. Dall WH. 1909. Report on a collection of shells from Peru, with a summary of the littoral marine mollusks of the Peruvian zoological province. Proceedings of the United States National Museum 37(1704):147–294 DOI 10.5479/si.00963801.37-1704.147. Dayrat B. 2009. Review of the current knowledge of the systematics of Onchidiidae (Mollusca: Gastropoda: Pulmonata) with a checklist of nominal species. Zootaxa 2068:1–26. d’Orbigny A. 1834–1847. Voyage dans l’Ame´rique me´ridionale (le Bre´sil, la re´publique orientale de l’Uruguay, la re´publique Argentine, la Patagonie, la re´publique du Chile, la re´publique de Bolivia, la re´publique du Pe´rou), exe´cute´ pendant les anne´es 1826, 1827, 1828, 1829, 1830, 1831, 1832, et 1833. Tome 5, Partie 3, Mollusques. Paris: P. Bertrand. 758 pp. d’Orbigny A. 1835–1843. Voyage dans l’Ame´rique Me´ridionale. Paris: P. Bertrand. Mollusques dates of publication: pp. 1–48 [1835], pp. 49–184 [1836], pp. 185–376 [1837], pp. 377–408 [1840], pp. 409–488 [1841], pp. 489–758, pls 1–85 [1846]. Fischer MA, Cervera JL. 2005a. Baptodoris peruviana (D’Orbigny, 1837) comb. nov., an alternative taxonomic placement for Doris peruviana (Gastropoda: Nudibranchia: Doridoidea). Journal of Conchology 38:513–528. Fischer MA, Cervera JL. 2005b. Checklist of the opisthobranchs (Mollusca: Gastropoda) from the Chilean coast deposited in the “Coleccio´n de Flora y Fauna Profesor Patricio Sa´nchez Reyes” from the “Pontificia Universidad Cato´lica de Chile.” Iberus 23(2):165–181. Fischer MA, van de Velde G, Roubos EW. 2007. Morphology, anatomy and histology of a new species of Flabellina Voigt, 1834 (Opisthobranchia: Aeolidoidea) from the Chilean coast. Animal Biology 57(4):363–391 DOI 10.1163/157075607782232116. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5):294–299. Gay C. 1854. Atlas de la Historia Fı´sica y Polı´tica de Chile. Paris: Imprenta de E. Thunot y Ca. Segundo Tomo. Gigoux EE. 1932. Sobre algunos caracoles terrestres de Atacama. Revista Chilena de Historia Natural 36(1):20–25. Gigoux EE. 1934. Los moluscos marinos de Atacama. Revista Chilena de Historia Natural 38:274–286. Gould AA. 1852. Mollusca & Shells, United States Exploring Expedition, vol. 12. Boston: Gould & Lincoln. xv + 510 pp. Gray JE. 1850. Figures of Molluscous Animals, Selected from Various Authors, Etched for the Use of Students, vol. 1–4. London: Longman, Brown, Green and Longmans. Guzma´n N, Saa´ S, Ortlieb L. 1998. Cata´logo descriptivo de los moluscos litorales (Gastropoda y Pelecypoda) de la zona de Antofagasta, 23 S (Chile). Estudios Oceanolo´gicos 17:17–86. Hermosillo A, Valde´s A. 2008. Two new species of opisthobranch mollusks from the tropical eastern Pacific. Proceedings of the California Academy of Sciences 59(13):521–532. Hupe´ H. 1854. Fauna Chilena: Moluscos, Historia fı´sica y polı´tica de Chile, 95 Zoologı´a, vol. 8. Paris: Gay. iii + 499 pp., 14 pls.

Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

17/20

Jensen KR. 2007. Biogeography of the Sacoglossa (Mollusca, Opisthobranchia). Bonner zoologische Beitra¨ge 55(3/4):255–281. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Mentjies P, Drummond A. 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647–1649 DOI 10.1093/bioinformatics/bts199. Labrı´n M, Guzma´n G, Sielfeld W. 2015. Ptero´podos thecosomados en el Pacı´fico suroriental frente a Caldera, Chile (Mollusca, Opistobranchiata: Euthecostomata y Pseudothecostomata). Latin American Journal of Aquatic Research 43(1):71–79 DOI 10.3856/vol43-issue1-fulltext-7. Lesson RP. 1831. Voyage autour du monde execute par ordre du roi sur la corvette de sa Majeste´, La Coquille, pendant les anne´es 1822, 1823, 1824 et 1825. Zoologie 2:239–455. MacFarland FM. 1909. The opisthobranchiate Mollusca of the Branner-Agassiz expedition to Brazil. Letland Stanford Junior University Publications 2:1–104. MacFarland FM. 1966. Studies of opisthobranch mollusks of the Pacific coast of North America. Memoirs California Academy of Sciences 6:1–546. Marcus E. 1959. Reports of the Lund University Chile Expedition 1948–1949, 36. Lamellariacea ˚ rsskrift (NF) 55:1–135. und Opisthobranchia. Lunds Universitets A Marcus E, Marcus E. 1967. American opisthobranch mollusks. Miami: Institute of Marine Sciences, 256 pp. Marincovich L. 1973. Intertidal mollusks of Iquique, Chile. Natural History Museum. Los Angeles County Science Bulletin 16:42–49. Martynov A, Schro¨dl M. 2011. Phylogeny and evolution of corambid nudibranchs (Mollusca: Gastropoda). Zoological Journal of the Linnean Society 163(2):585–604 DOI 10.1111/zoj.2011.163.issue-2. Millen S, Schro¨dl M, Vargas N, Indacochea A. 1994. A new species of Okenia (Nudipleura: Doridacea) from the Peruvian faunal province. The Veliger 37:312–318. Miquel SE, Araya JF. 2013. A new Charopidae from Chile and Argentina, Stephacharopa calderaensis n. gen. and n. sp., with remarks on the taxonomy of the genus Stephadiscus Hylton Scott 1981 (Mollusca: Gastropoda Pulmonata). Archiv fu¨r Molluskenkunde 142(2):227–235 DOI 10.1127/arch.moll/1869-0963/142/227-235. Muniaı´n C, Valde´s A, Ortea J. 1996. Redescription of Tyrinna nobilis Bergh, 1898 (Opisthobranchia: Chromodorididae) from Patagonia, Argentina. Journal of Molluscan Studies 62(3):265–273 DOI 10.1093/mollus/62.3.265. Nakamura K. 2006. New records of opistobranch mollusks from the Guayaquil marine ecoregion: northern Peru´. The Festivus 38:75–83. Nakamura K. 2007. Especies bento´nicas de Opisthobranchia (Mollusca: Gastropoda) presents en el litoral del norte peruano. Revista Peruana de Biologı´a 13(3):255–257 DOI 10.15381/rpb.v13i3.2352. Odhner NH. 1926. Die Opisthobranchien. Further Zoological Results of the Swedish Antarctic Expedition 1901–1903 2:1–100. Osorio C. 2012. Nueva especie del ge´nero Liotia (Gastropoda: Trochoidea: Liotiidae) del Pacı´fico sur oriental, norte de Chile. Revista de Biologı´a Marina y Oceanografı´a 47(3):407–411 DOI 10.4067/S0718-19572012000300004.

Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

18/20

Padula V, Wirtz P, Schro¨dl M. 2014. Heterobranch sea slugs (Mollusca: Gastropoda) from Ascension Island, South Atlantic Ocean. Journal of the Marine Biological Association of the United Kingdom 1–10 DOI 10.1017/S0025315414000575. Philippi RA. 1860. Viage [sic] al Desierto de Atacama, hecho de orden del Gobierno de Chile en el verano 1853–1854. Halle: Sajonia, 254 pp. Pilsbry HA. 1896. Manual of Conchology Philadelphia 16:175–226. Rehder HA. 1945. The Chilean species of the molluscan genus Peronaeus (Bulimulidae). Revista Chilena de Historia Natural 48(1):102–107. Reiswig H, Araya JF. 2014. A review of the Hexactinellida (Porifera) of Chile, with the first record of Caulophacus Schulze, 1885 (Lyssacinosida: Rossellidae) from the Southeastern Pacific Ocean. Zootaxa 3889(3):414–428 DOI 10.11646/zootaxa.3889.3.4. Risso A. 1826. Histoire Naturelle des Principales Productions de l’Europe Me´ridionale et Particulie`rement de Celles des Environs de Nice et des Alpes Maritimes Histoire Naturelle des Principales Productions de l’Europe Me´ridionale. Paris: F.-G. Levrault. 4 [iii] + vii + 439 pp., 12 pls. Rosenfeld S, Aldea C. 2010. Onchidella marginata (Couthouy en Gould, 1852): antecedentes de la especie. Amici Molluscarum 18:35–37. Schro¨dl M. 1996a. Nudibranchia y Sacoglossa de Chile: Morfologı´a exterior y distribucio´n. Gayana 60:17–62. Schro¨dl M. 1996b. Janolus rebeccae, a new species of arminacean nudibranchs from northern Chile. Spixiana 19(3):293–300. Schro¨dl M. 1997. Range extensions of Magellanic Nudibranchs (Opisthobranchia) into the Peruvian faunal province. The Veliger 40(1):38–42. Schro¨dl M. 1999. The genus Berthella Blainville, 1825 (Notaspidea, Pleurobranchidae) from Magellanic waters. Journal of Molluscan Studies 65:399–409. Schro¨dl M. 2000a. Revision of the nudibranch genus Cadlina (Gastropoda: Opisthobranchia) from the Southern Ocean. Journal of the Marine Biological Association of the United Kingdom 80(2):299–309 DOI 10.1017/S0025315499001873. Schro¨dl M. 2000b. Taxonomic revision of the common South American nudibranch Anisodoris fontaini (d’Orbigny, 1837), with discussion of its systematic placement. Journal of Molluscan Studies 66(1):69–82 DOI 10.1093/mollus/66.1.69. Schro¨dl M. 2003. Sea Slugs of Southern South America. Hackenheim: Conch Books, 165 pp. Schro¨dl M. 2009. Opisthobranchia–sea slugs. In: Ha¨ussermann V, Fo¨rsterra G, eds. Fauna Marina Bento´nica de la Patagonia Chilena. Chile: Nature in Focus, 505–542. Schro¨dl M, Grau JH. 2006. Nudibranchia from the remote southern Chilean Guamblin and Ipu´n Islands (Chonos Archipelago, 44–45  S), with redescription of Chilean Rostanga pulchra MacFarland, 1905. Revista Chilena de Historia Natural 79:3–12. Schro¨dl M, Hooker Y. 2014. Sea slugs of Peru: Peruvian-Chilean faunal elements. Spixiana 37(1):45–59. Schro¨dl M, Millen SV. 2001. Revision of the nudibranch gastropod genus Tyrinna Bergh, 1898 (Nudibranchia: Doridoidea). Journal of Natural History 35(8):1143–1171 DOI 10.1080/00222930152434472. Schro¨dl M, Alarco´n MA, Bedrin˜ana LR, Bravo FJ, Bustamante CM, Carvalho R, Fo¨rsterra G, Ha¨ussermann V, Salmen A. 2005. Nudipleura (Gastropoda: Opisthobranchia) from the southern Chilean Comau Fjord, with redescription of Polycera priva Marcus, 1959. Vita Malacologica 3:23–33.

Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

19/20

Sowerby GB. 1832. The Conchological Illustrations or, Coloured Figures of all the Hitherto Unfigured Recent Shells. London. Sowerby GB. 1833. New species of shells collected by Mr. Cuming on the Western coast of South America and among the Islands of the South Pacific Ocean. They were accompanied by characters from the pen of Mr. G. B. Sowerby. Proceedings of the Zoological Society of London 1833:34–38. Sowerby GB II. 1840. Characters of new species of Mollusca and Conchifera, collected by Mr. Cumming. In: Broderip WJ, Sowerby GB I, eds. Proceedings of the Malacological Society of London for 1832, 1833. London: James Sowerby. Pp. 25–33, 50–61, 104–108, 124–126 [1832]; 173–179, 194–202 [1833]. Uribe RA, Pacheco AS. 2012. First record of Spurilla neapolitana (Mollusca: Nudibranchia: Aeolidiidae) on the central coast of Peru (Humboldt Current Upwelling Ecosystem). Marine Biodiversity Records 5(3):e14 DOI 10.1017/S1755267211001138. Uribe RA, Nakamura K, Indacochea A, Pacheco AS, Hooker Y, Schro¨dl M. 2013. A review on the diversity and distribution of opisthobranch gastropods from Peru, with the addition of three new records. Spixiana 36(1):43–60. Uribe R, Rubio J, Carbajal P, Berru´ P. 2014. Invertebrados marinos bento´nicos del litoral de la Regio´n A´ncash, Peru´. Boletı´n Instituto del Mar del Peru´ 28(1–2):136–293. Valde´s A, Muniaı´n C. 2002. Revision and taxonomic reassessment of Magellanic species assigned to Anisodoris Bergh, 1898 (Nudibranchia: Doridoidea). Journal of Molluscan Studies 68(4):345–351 DOI 10.1093/mollus/68.4.345. Valdovinos C. 1999. Biodiversidad de moluscos chilenos: Base de datos taxono´mica y distribucional. Gayana 63(2):111–164.

Araya and Valdés (2016), PeerJ, DOI 10.7717/peerj.1963

20/20