Cell Stress and Chaperones DOI 10.1007/s12192-014-0502-y
ORIGINAL PAPER
Silica nanoparticles induce oxidative stress and inflammation of human peripheral blood mononuclear cells Alvaro Mendoza & Jose A. Torres-Hernandez & Jeffrey G. Ault & Joan H. Pedersen-Lane & Donghong Gao & David A. Lawrence
Received: 28 February 2013 / Revised: 31 January 2014 / Accepted: 2 February 2014 # Cell Stress Society International 2014
Abstract In the present study, the effects of 10- or 100-nm silica oxide (SiO2) NPs on human peripheral blood mononuclear cells (PBMC) were examined. Cytotoxic effects and oxidative stress effects, including glutathione (GSH) depletion, the formation of protein radical species, and proinflammatory cytokine responses, were measured. PBMC exposed to 10-nm NP concentrations from 50 to 4,000 ppm showed concentration-response increases in cell death; whereas, for 100-nm NPs, PBMC viability was not lost at
ORIGINAL PAPER
Silica nanoparticles induce oxidative stress and inflammation of human peripheral blood mononuclear cells Alvaro Mendoza & Jose A. Torres-Hernandez & Jeffrey G. Ault & Joan H. Pedersen-Lane & Donghong Gao & David A. Lawrence
Received: 28 February 2013 / Revised: 31 January 2014 / Accepted: 2 February 2014 # Cell Stress Society International 2014
Abstract In the present study, the effects of 10- or 100-nm silica oxide (SiO2) NPs on human peripheral blood mononuclear cells (PBMC) were examined. Cytotoxic effects and oxidative stress effects, including glutathione (GSH) depletion, the formation of protein radical species, and proinflammatory cytokine responses, were measured. PBMC exposed to 10-nm NP concentrations from 50 to 4,000 ppm showed concentration-response increases in cell death; whereas, for 100-nm NPs, PBMC viability was not lost at
