Accepted Manuscript Title: SOLUBLE HLA-G IN PREGNANCIES COMPLICATED BY AUTOIMMUNE RHEUMATIC DISEASES Author: Fausta Beneventi Carla Badulli Elena Locatelli Roberto Caporali V´eronique Ramoni Chiara Cavagnoli Margherita Simonetta Giulia Garbin Carmine Tinelli Claudia Alpini CarloMaurizio Montecucco Miryam Martinetti Arsenio Spinillo PII: DOI: Reference:
S0165-0378(15)00073-X http://dx.doi.org/doi:10.1016/j.jri.2015.04.005 JRI 2328
To appear in:
Journal
Received date: Revised date: Accepted date:
5-2-2015 16-4-2015 27-4-2015
of
Reproductive
Immunology
Please cite this article as: Beneventi, F., Badulli, C., Locatelli, E., Caporali, R., Ramoni, V., Cavagnoli, C., Simonetta, M., Garbin, G., Tinelli, C., Alpini, C., Montecucco, C.M., Martinetti, M., Spinillo, A.,SOLUBLE HLA-G IN PREGNANCIES COMPLICATED BY AUTOIMMUNE RHEUMATIC DISEASES, Journal of Reproductive Immunology (2015), http://dx.doi.org/10.1016/j.jri.2015.04.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Highlights for review
Maternal sHLA-G levels in rheumatic and healthy pregnant women.
Ac ce p
te
d
M
an
us
cr
Maternal and fetal HLA-G polymorphism in rheumatic and healthy pregnant women
ip t
Fetal sHLA-G levels in the cord blood of rheumatic and healthy pregnant women.
1
Page 1 of 23
SOLUBLE
HLA-G
IN
PREGNANCIES
COMPLICATED
BY
AUTOIMMUNE
RHEUMATIC DISEASES Fausta Beneventia, Carla Badullic, Elena Locatellia , Roberto Caporalib, Véronique Ramonib, Chiara Cavagnolia, Margherita Simonettaa, Giulia Garbinc, Carmine Tinellid, Claudia
a
cr
ip t
Alpini°, CarloMaurizio Montecuccob, Miryam Martinetti c, Arsenio Spinilloa.
Department of Obstetrics and Gynecology, IRCCS Foundation Policlinico San Matteo and
b
us
University of Pavia, Pavia, Italy;
Division of Rheumatology, IRCCS Foundation Policlinico San Matteo and University of Pavia,
Pavia, Italy
Immunogenetics Laboratory, Immunohematology and Transfusion Center, IRCCS Foundation
an
c
Policlinico San Matteo, Pavia, Italy;
Clinical Epidemiology and Biometric Unit, IRCCS Foundation Policlinico San Matteo, Pavia,
M
d
Italy;
°Clinical Chemistry Laboratory, IRCCS Foundation Policlinico San Matteo, Pavia, Italy
Ac ce p
Abstract
te
d
email corresponding author: [email protected]
Autoimmune rheumatic diseases in pregnancies are associated with increased adverse obstetric outcomes. We compared maternal soluble human leucocyte antigen-G (sHLA-G) blood levels in subjects with a rheumatic disease preexisting pregnancy and unaffected controls. Third-trimester blood maternal sHLA-G concentrations were significantly higher in subjects with rheumatic diseases than in controls (mean 93.1 ng/ml [SD 42.1] vs 58.1 ng/ml [SD 96.3], p= 0.003). Cord blood sHLA-G concentrations were significantly higher in rheumatic disease than in those born to control mothers (median 41.2 ng/ml [IQR: 3.3–44.0] vs 17.9 ng/ml [IQR: 17.2–88.1], p = 0.007). A strict positive correlation (r= 0.88, p
S0165-0378(15)00073-X http://dx.doi.org/doi:10.1016/j.jri.2015.04.005 JRI 2328
To appear in:
Journal
Received date: Revised date: Accepted date:
5-2-2015 16-4-2015 27-4-2015
of
Reproductive
Immunology
Please cite this article as: Beneventi, F., Badulli, C., Locatelli, E., Caporali, R., Ramoni, V., Cavagnoli, C., Simonetta, M., Garbin, G., Tinelli, C., Alpini, C., Montecucco, C.M., Martinetti, M., Spinillo, A.,SOLUBLE HLA-G IN PREGNANCIES COMPLICATED BY AUTOIMMUNE RHEUMATIC DISEASES, Journal of Reproductive Immunology (2015), http://dx.doi.org/10.1016/j.jri.2015.04.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Highlights for review
Maternal sHLA-G levels in rheumatic and healthy pregnant women.
Ac ce p
te
d
M
an
us
cr
Maternal and fetal HLA-G polymorphism in rheumatic and healthy pregnant women
ip t
Fetal sHLA-G levels in the cord blood of rheumatic and healthy pregnant women.
1
Page 1 of 23
SOLUBLE
HLA-G
IN
PREGNANCIES
COMPLICATED
BY
AUTOIMMUNE
RHEUMATIC DISEASES Fausta Beneventia, Carla Badullic, Elena Locatellia , Roberto Caporalib, Véronique Ramonib, Chiara Cavagnolia, Margherita Simonettaa, Giulia Garbinc, Carmine Tinellid, Claudia
a
cr
ip t
Alpini°, CarloMaurizio Montecuccob, Miryam Martinetti c, Arsenio Spinilloa.
Department of Obstetrics and Gynecology, IRCCS Foundation Policlinico San Matteo and
b
us
University of Pavia, Pavia, Italy;
Division of Rheumatology, IRCCS Foundation Policlinico San Matteo and University of Pavia,
Pavia, Italy
Immunogenetics Laboratory, Immunohematology and Transfusion Center, IRCCS Foundation
an
c
Policlinico San Matteo, Pavia, Italy;
Clinical Epidemiology and Biometric Unit, IRCCS Foundation Policlinico San Matteo, Pavia,
M
d
Italy;
°Clinical Chemistry Laboratory, IRCCS Foundation Policlinico San Matteo, Pavia, Italy
Ac ce p
Abstract
te
d
email corresponding author: [email protected]
Autoimmune rheumatic diseases in pregnancies are associated with increased adverse obstetric outcomes. We compared maternal soluble human leucocyte antigen-G (sHLA-G) blood levels in subjects with a rheumatic disease preexisting pregnancy and unaffected controls. Third-trimester blood maternal sHLA-G concentrations were significantly higher in subjects with rheumatic diseases than in controls (mean 93.1 ng/ml [SD 42.1] vs 58.1 ng/ml [SD 96.3], p= 0.003). Cord blood sHLA-G concentrations were significantly higher in rheumatic disease than in those born to control mothers (median 41.2 ng/ml [IQR: 3.3–44.0] vs 17.9 ng/ml [IQR: 17.2–88.1], p = 0.007). A strict positive correlation (r= 0.88, p
