Exp. Anim., 26 (2), 161-167, •\
1977
Note•\
Spontaneous
Lesions Used
Keizo
Laboratory
MAITA,
Detected
in Toxicity
Hiroshi
in
the
Beagles
Studies.
MASUDA
and
Yoshio
SUZUKI
Animal Science and Toxicology Laboratories of Sankyo 717 Horikoshi, Fukuroi Shizuoka 437, Japan. (Received
for publication
: November
Co . Ltd.,
29, 1976)
Four hundred and twenty beagles 1 to 4 years of age were examined for spontaneous pathological changes. In the liver were seen fine granular gallstones (87.5%), granulomas (59.9%), intranuclear hyaline inclusions (52.6%), lipofuscin deposition (50%) and eosinophilic inclusions (3.7%) in the cytoplasm of hepatocytes and parenchymal necrosis at the base of the ligaments (7 .3%). Intranuclear hyaline inclusions and lipof uscin deposition were more frequently seen in dogs after being subjected to tests for more than 13 weeks . Besides, hyperplasia of follicular epithelial cells of the thyroid gland (46 .3%), Gandy-Gamna like bodies of the splenic capsule (43.1%), small cysts in the anterior lobe of the hypophysis (35.1%) and prostatitis (13.7%) were observed. Lesions due to migration of T oxocara canis larvae were detected in the liver , kidney, lung and other organs from 33.1% of cases examined.
With
development
more
detailed
ound
have
Among to
causing tion
been lot
some
though reared The
effects of
changes
of
420
raised
and
used
Materials mined
here
known
the
animals
deals in
which
in this
consisted
of
age.
weaned
at 2 months
of
age
mental
were
and
other
prevent
were
born,
chow gm/day
Pups and
exaand
205 were
divided
in
At
Oriental water
recieved
leptospirosis
2
a
pen
Co.,
supplied
by
3
a
combined
of
months
was
windows to
fed
biscuit
Tokyo) an
250
automatic of
age
vaccine
infectious
2
environ-
mesh-net were
and
age
pens
Dogs
(Dohyvac,
of
the
Yeast
canine
surface
The
fine
by
with
months
and a
1.5
a
6
rearing
5•Ž
with
At
of
to
of 24 •}
mosquitoes.
and
pen
and
equipped
transferred
covered
distemper,
a
f loorheating.
at
nst
sexes,
providing
temperature
and
same
was
35•Ž.
were
(DB,
of
housed
without
system. animals
8
which
of
controled
spontaneous
or
system
temperature
3 m
usually
7
of
f loorheating
animals
of 215 males
1 to 4 years
was
half
by
: Animals
females
group a
all
laboratory.
methods
of
samples,
environments.
liver
groups
evalua-
are
with
the
beagles
and
3 m
changes
test
controled
occurring
each
animals.
are
during
the
paper
organs
test
of various
in strictly
into
backgr-
spontaneous
problems
most
present
on beagles
of
studies,
on the
required
animals,
a
of toxic
toxicity
inf ormations
these
have
of
all agai-
hepatitis
Philips-Duphar,
162
Netherland). dazole
For (Sankyo,
was
given
orally
at
for
and
3
a
8
were
After
at
observation,
and
in
were
periodic-acid
Schif, blue,
acid-fast
and
von
Results % of
examined.
brown
some
or
cases
derably
over
diameter.
no
a 1
opening
3
of
fragile stones
age
had 2
seemed
mechanical
1.5
by
the
cystic
but
5mm
in
duct,
died
at of
a
8
granulomas
histiocytes
lymphocytes slightly
small
with and
increased
a
small
collagen
of
as were
of cases examitested
rather
for more
examined
fresh
having
or
or yellow
sizes
of
millet
the
of
the
lesions
in depth.
Organized
liver.
On
revealed
the
cut
ramification
reaching
1 to 1.5cm
small necrotic
lesions
It is noticeable hepatic
more frequently not
obedient
7
Eosinophilic
that
such spo-
necrosis
seemed
to occur
in either
animals
exciting
in handling frequently
circular
or
those
to long
time-
or cubic
cytoplas-
well
as
mic inclusion bodies were seen singly or in pairs in hepatocytes from 3.7% of cases
seen
in
examined.
number
neutrophils
damage
cytoplasmic
as well as white
being subjected holding.
obstr-
consisted
and
of
substa-
without
animals
parenchyma
and
the
jaundice. Very
axis
was seen, and found
into the
ntaneous
had
size
but
of 50 /
nodules
xic drugs.
generally obstructions,
cm
13
in the deep portion of the parenchyma resembled to those produced by hepatoto-
consito
long nuclear
membrane
ligament
surface
87.5 were
which
0.5
lar
1),
stones
year-old-female,
by of
uctive
years
(Fig
3). freq-
grains to soy beans were seen at the base of the left and right coronary or triangu-
fuchsin
them
the
In 7.3 % of cases necrotic
in
along
old hemorrhages
stain.
of
crystals
These
produce
stone
2 and
in 7.2) sect-
found
Most
black
large
while
were
(Fig, more
for more than
compressed
in the
or
than 13 weeks, though the grade of deposition varied in each case (Fig. 4).
trichrome,
calcium
: Gallstones
cases
fine
Kossa's
cubic
a large inclusion body
nce at the periphery
frequently
fixed
carbol
with
ned lipof uscin
after
(pH
Masson's
elongated
these
hepatocytes
occurred
tested
In hepatocytes
hematoxylin-eosin,
f,
Nuclei having
substances.
each
carotid
were
Verhoeff's
in animals
in
examined
bodies
weeks.
around
bodies,
found
uently
of
Five ƒÊcm
with
2
25mg/kg
formalin
52.6%
in the nuclear
collected
paraffin.
stained
Prussian
of from
they
of cases
with
dose
were
buffered
embedded
ions
a
from
various
anesthetized
organs
neutral
and
termination
thoroughly
10
or
the were
hyaline were
inclusion
for to
in or
rectangular,
were
in
automatic
served
pentobarbital
gross
housed
acclimatization
After
Main
lighting
hepatocytes
present
Such inclusion
conditioned
and
degenerated
granulomas. Intranuclear
given
and
A few
occasionally
age,
animals
with
were
exanguinated
%
rooms
cages
they
artery.
were
of
over
7 : 30pm,
they
experiment,
and
(Fig,
2).
parben-
humidity
to
weeks,
sodium
the liver of 59.9% of cases examined
capsule
were
or
test
alminium
experiments.
to
age
to
supply.
4
parben-
30mg/kg
weeks
containing
of
7 : 30am
or
of 4
Tokyo)
55•}1%
individual water
or
month.
months
24•}1•Ž,
from
and
CLEA,
transferred
at
syrup
dose
2
biscuits
each At
in
at
(Medical,
during
control in
daily
days
thereafter
dazole
parasite
Tokyo)
of
They
were 2 to l0pm in diam-
163
eter
without
halo
and
were
positive
for
periodic acid Schif f and Verhoef f's carbol fuchsin acid-fast stain (Fig. 5). Bilateral epithelial
hyperplasia cells
was
of the
seen
follicular
in the
and light
nucleus
abundant
cytoplam
iferation
and
tissues
and
showed
abnormally
a nodular
compressed
was also seen
frequently
prol-
appeared
small
or brown
to millet grains most
in the but
they
being
caudal
thickened increase
parts
and diffuse
surface
not extensignificantly showed
small
deposit
an
foci
of brown
(Fig. 8). also seen
Occasionally, calcification along collagen fibers.
incidences
of these
ected dams
according or sires
which had experiments, Cysts found
to types from
the
were
was While
not
aff-
histiocytes
appeared
(Fig. 10).
or
their
debris
encountered
in
eggs of the parasite examinations.
lobe of the
hypoph-
lesions
Fragments
were
of
frequently
lesions,
while
were detected
: Many
investigations
on beagles
; breeding
no
in fecal have systems
[3] as well as hematology
[1, 2,
5, 10] and biochemistry [4, 7, 9]. Pathological backgrounds, however, remain unclear despite of the importance in reading effects
of
given
they
experimental
the results
treatment.
presented
here
could
to all tests using beagles,
would provide
some
informations
to
pathologist engaged in the subject. In the present study there were many kinds
of
changes
them gallstones
cells of the anterior
idered
a small
round
of connective
studied
ysis from 35.1 % of cases examined. The wall of these cysts consisted of flattened lobe containing
small
Discussion
breeding
were
to be
granulomatous
tissues
not be applicable
20 to 200 dim in diameter
and
these
of
the lung In other
small
by a thin layer
Although
colony,
too
sizes
revealed that these by a number of
larvae
and diets
lymin the to be
having
were
Histology infiltrated
encapsulated
been
liver,
mesenteric
nodules
lesions
seen grossly. lesions were
of experiments,
not been subjected to any rarely exhibited such changes.
in the anterior
the
in 33.1
lungs,
others. The lesions and spleen appeared
or brown
organs
of
pigments, and a number of macrophages were seen to phagocytize the pigments
changes
pancreas,
while of Tox-
detected
in the
cells, and sometimes 2
only within
of the capsule with
spleen,
eosinophils,
periphery cut
usually
of collagen
hemorrhage
poppy
attaining
Microscopically,
were
millet grains to soy beans and lesions accompanied atelectasis.
On the
the capsule
ding deeply.
males,
like
were seen to be localized
or beneath
larvae
examined,
kidneys,
of
bodies
sometimes
or 3cm long (Fig. 7).
13.7 %
remained unclear. caused by migration
canis
cases
was seen in the
white
in size were found grossly
frequently
of the capsule,
nodules
from
amorphous
was
Gandy-Gamna bodies in the splenic capsule of 43.1 % of cases examined. Usually, a few white
inflammation
pathogenesis Damages ocara
basophilic
mitosis
rare. There
(Fig. 9).
Chronic
phnodes and liver, kidneys
hyperplasia
but
materials
of
neighbouring
(Fig. 6). Adenomatous
of weakly
prostates
thyroid
glands from 46.3 % of cases examined. Hyperplastic cells having a larger and light nucleus
amount
damage.
in the
liver.
Among
seemed not to cause serious
However,
granulomas
to be of importance
were
cons-
in evaluating
164
effects
of
such with of
experimental
lesions
treatments,
were
degeneration
and
hepatocytes Intranuclear
scin more
of
more
than
were
known
to
of
dogs
process
bodies
in
13
study
might
during
experiments.
appear
cells
asmic
that
in
stress.
The
which
after
parasitic study
to
experiments
as suggested
after
as gestatliving body
in
might
stimulation lesions
might
initiated
during
mechanism
present
larvae of the
matured
present
eggs
such
that parts
be
in the
stresses
aging
the
to
the
T oxocara stress
suggested or other
by larvae
changes
by
obse-
be induced migrate
by
through by
a
Johnson.
stresses
found
correspond
in
et
li-
[1]
to •gintracytopl-
nonglycogenic al
inclusion
lysosomal
rved
of
some
References
Murti
such
and
migrate by
for
dogs,
inclusions
might
by
aged
accelerated
PAS-positive
described
these
in
u-
seen
tested
examined be
lipof
were
Since
Intracytoplasmic ver
and
animals
weeks.
excretion
exposed
muscles
hepatocytes
frequently
ion,
necrosis
them.
hyaline
deposition
cell
for
being
associated
single
around
tive
since
sometimes
[8].
globule•h who
alterations
of
21.
suggested
bodies
Andersen, A. C. and Gee, W. (1957). Blood values in the beagle. Veterinarian, 18, 16-
[2]
represented
hepatocytes
due
Andersen, A. C. and Gee, W. (1958). Normal blood values in the beagle. Vet. Med., 53 135-139.
to
nonspecific
relation
slight
to
the
cases the
thyroid
that
examined
as
in
lobe
35.1%
enital
follicular
were
we
could
and
of
assumed
sires
of
the
Prostatitis with
some
urine
tests.
see
fluctuation
in
occurbe
[4]
cong-
was
no
changes.
bodies 43.1
%
to
be
of
such
in
cases
[6]
with
in
dams
[7]
colony. to
be
of
Iowa,
USA.
correlated
parameters
T oxocara of
cases
ication [6]
canis
larvae
suggests of
described
this
much parasitic that
some
by detected
dogs
of
in
33.1% of
infection.
R.H. and Trum, B. F. (1964). of dogs for biochemical reseaCare, 14, 226-234.
age. J. Gerontol.,
Johnson,
20, 131-138.
R.M., Andersen,
A.D. and Gee, W.
Parasitism in an established dog Lab. Animal Care, 13, 731-736.
McKelvie,
D.H., Powers,
S. and McKim, F. for on
posi-
Amer. J, vet. Res., 27, 1405-1512.
Murti, G.S. and Borgmann, R. (1965). Intrahepatic periodic acid-Schiff-positive nonglycogenic
erad-
Their
Johnson became
Press,
in
migration
difficulty
A. C.,
University
(1966). Microanalytical procedures blood chemistry long-term study
[8] caused
Dog, Andersen, State
Dougherty, J.H. and Resenblatt, L.S. (1965). Changes in the hemogram of the beagle
beagles. Inflammation
Iowa
Charlewood, Acquisition
(1963). kennel.
since
cases
The
with
exa-
related
editor,
rch. Lab. Animal [5]
observed
experiments,
breeding seemed
cysts
there two
during
rarely
of
to
Andersen, A. C. and Goldman, M. (1970). Growth and development. In The Beagle as an Experimental
46.3%
hypophysis
the
capsule
cells
small
whereas
like
handlings
in
considered
Gandy-Gamna
forced
as
the
were
mined
seen
well
between
splenic
epithelial
were
of
defects,
correlation
the
in
[3]
of
anterior
ring
caused
hepatotoxicity.
Hyperplasia of
damage
globules
histochemical
in
canine
characterization.
liver: Amer.
J. vet. Res., 26, 63-67. [9]
Nagode,
L. A., Walter,
J. F. and walter,
F.
165
L. (1966). Enzymatic tissues. [10]
activities
of canine
Soave, O.
A. and
Boyle,
ned
C. C. (1965).
A
Explanation Fig.
1
Fine
crystaline
old Fig.
2
gallstones
of
of
lipof of
uscin
stain. •~
few
incluison
Nodular
Fig.
a and
hepat-
stain. •~ deposit
in
7
Fig.
8
mar-
Fig.
9
acid-
of
hepatocytes bodies
hepatocytes.
(arrowere
26-month-old Schiff
reaction. •~
10
thyroid
body
epit-
gland.
28sta-
figure
Photo.
7.
of
fibers
the
nodules
shown increased
with
cyst
abundant
of
hemo-
Prussian
the
female,
blue
hypophysis.
26-
hematoxylin-eosin
100.
larvae
in. •~
the
(arrows).
Inflammatory
fem-
in male.
100.
Congenital
month-old
nodules
Significantly
deposits
seen
like
16-month-old
Microscopic
nis
follicular
hematoxylin-eosin
capsule.
month-old
Fig.
the
Gandy-Gamna
stain. •~ intracytoplasmic
inclusion
of
reaction. •~ fe-
f uchsin
dogs.
200.
siderin
cytop-
of
male,
collagen
26-month-old carbol
bodies
periodic-acid
cells
in
1000.
the
conditio-
15, 359-362.
hyperplasia
in. •~
25-month-old
globular
Two some
in
Care,
month-old
400.
and
ws).
body nucleus
hepatocytes.
Verhoeff's
Large
ale,
a
lymphocytes.
chromatins.
Diffuse
fast
with by
enlarged
hamatoxylin-eosin
in
6
splenic
female,
lasm
5
Fig.
hematoxylin-eosin
inclusion with
gination
Fig.
liver
female,
Intranuclear
male,
Animal
of
laboratory
Figures
400.
ocyte
4
the
surrounded
stain. •~
Fig.
18-month-
hemograms
non-conditioned
helial
Granuloma
13-month-old
3
.
of
of the
and
Lab.
male.
histiocytes
Fig.
comparison
Amer. J. vet. Res., 27, 1385-1393.
reactions in
cortex
male,
of
to
T oxocara
the
kidney.
ca18-
hematoxylin-eosin
sta-
200.
400.
毒 性 試 験 に 用 い られ た ビ ー グ ル 犬 の 偶 発 病 変 真 板 敬 三 ・増 田
裕 ・鈴 木 善 雄
三共株式会社安全性試験センター 1才 か ら4才 の ビー グル犬420頭
について偶発病変を
甲状 腺 で 小胞 上 皮 細 胞 の 過 形 成 が46.3%に,ま
検 索 した と ころ,肝 に お い て胆 石 が87%,内
芽 腫 が59.9
膜 のGandy-Gamna氏
%,肝
ポ フス チ ン
体 前 葉 に おけ る小 の う腫 が35.1%見
細 胞 の硝 子 様 核 内封 入 体 が52.6%,リ
沈 着 が50%,好 ま た,靱
酸 性 細 胞 質 内 封 入 体 が3.7%に
帯 付 着 部 の実 質 壊 死 が3.7%に
認 め られ,
み られ た 。肝 細
胞 の硝 子 様 核 内 封 入 体 お よび リポ フス チ ン沈 着 は,実 験 期 間が13週 間 以 上 の場 合 高 率 に 認 め られ た 。肝 以 外 では,
た,脾 被
小 体様 の 結 節 が43.1%に,下 出 され,さ
垂
らに 雄 の
13.7%に 前 立 腺 炎 が 認 め られ た 。 ま た,犬 回 虫 の幼 虫が 体 内移 行 す る際 に 生 じた 病 変が,33.1%の 肺 等 に 認 め られ た 。
犬 の肝 ・腎 ・
166
167
1977
Note•\
Spontaneous
Lesions Used
Keizo
Laboratory
MAITA,
Detected
in Toxicity
Hiroshi
in
the
Beagles
Studies.
MASUDA
and
Yoshio
SUZUKI
Animal Science and Toxicology Laboratories of Sankyo 717 Horikoshi, Fukuroi Shizuoka 437, Japan. (Received
for publication
: November
Co . Ltd.,
29, 1976)
Four hundred and twenty beagles 1 to 4 years of age were examined for spontaneous pathological changes. In the liver were seen fine granular gallstones (87.5%), granulomas (59.9%), intranuclear hyaline inclusions (52.6%), lipofuscin deposition (50%) and eosinophilic inclusions (3.7%) in the cytoplasm of hepatocytes and parenchymal necrosis at the base of the ligaments (7 .3%). Intranuclear hyaline inclusions and lipof uscin deposition were more frequently seen in dogs after being subjected to tests for more than 13 weeks . Besides, hyperplasia of follicular epithelial cells of the thyroid gland (46 .3%), Gandy-Gamna like bodies of the splenic capsule (43.1%), small cysts in the anterior lobe of the hypophysis (35.1%) and prostatitis (13.7%) were observed. Lesions due to migration of T oxocara canis larvae were detected in the liver , kidney, lung and other organs from 33.1% of cases examined.
With
development
more
detailed
ound
have
Among to
causing tion
been lot
some
though reared The
effects of
changes
of
420
raised
and
used
Materials mined
here
known
the
animals
deals in
which
in this
consisted
of
age.
weaned
at 2 months
of
age
mental
were
and
other
prevent
were
born,
chow gm/day
Pups and
exaand
205 were
divided
in
At
Oriental water
recieved
leptospirosis
2
a
pen
Co.,
supplied
by
3
a
combined
of
months
was
windows to
fed
biscuit
Tokyo) an
250
automatic of
age
vaccine
infectious
2
environ-
mesh-net were
and
age
pens
Dogs
(Dohyvac,
of
the
Yeast
canine
surface
The
fine
by
with
months
and a
1.5
a
6
rearing
5•Ž
with
At
of
to
of 24 •}
mosquitoes.
and
pen
and
equipped
transferred
covered
distemper,
a
f loorheating.
at
nst
sexes,
providing
temperature
and
same
was
35•Ž.
were
(DB,
of
housed
without
system. animals
8
which
of
controled
spontaneous
or
system
temperature
3 m
usually
7
of
f loorheating
animals
of 215 males
1 to 4 years
was
half
by
: Animals
females
group a
all
laboratory.
methods
of
samples,
environments.
liver
groups
evalua-
are
with
the
beagles
and
3 m
changes
test
controled
occurring
each
animals.
are
during
the
paper
organs
test
of various
in strictly
into
backgr-
spontaneous
problems
most
present
on beagles
of
studies,
on the
required
animals,
a
of toxic
toxicity
inf ormations
these
have
of
all agai-
hepatitis
Philips-Duphar,
162
Netherland). dazole
For (Sankyo,
was
given
orally
at
for
and
3
a
8
were
After
at
observation,
and
in
were
periodic-acid
Schif, blue,
acid-fast
and
von
Results % of
examined.
brown
some
or
cases
derably
over
diameter.
no
a 1
opening
3
of
fragile stones
age
had 2
seemed
mechanical
1.5
by
the
cystic
but
5mm
in
duct,
died
at of
a
8
granulomas
histiocytes
lymphocytes slightly
small
with and
increased
a
small
collagen
of
as were
of cases examitested
rather
for more
examined
fresh
having
or
or yellow
sizes
of
millet
the
of
the
lesions
in depth.
Organized
liver.
On
revealed
the
cut
ramification
reaching
1 to 1.5cm
small necrotic
lesions
It is noticeable hepatic
more frequently not
obedient
7
Eosinophilic
that
such spo-
necrosis
seemed
to occur
in either
animals
exciting
in handling frequently
circular
or
those
to long
time-
or cubic
cytoplas-
well
as
mic inclusion bodies were seen singly or in pairs in hepatocytes from 3.7% of cases
seen
in
examined.
number
neutrophils
damage
cytoplasmic
as well as white
being subjected holding.
obstr-
consisted
and
of
substa-
without
animals
parenchyma
and
the
jaundice. Very
axis
was seen, and found
into the
ntaneous
had
size
but
of 50 /
nodules
xic drugs.
generally obstructions,
cm
13
in the deep portion of the parenchyma resembled to those produced by hepatoto-
consito
long nuclear
membrane
ligament
surface
87.5 were
which
0.5
lar
1),
stones
year-old-female,
by of
uctive
years
(Fig
3). freq-
grains to soy beans were seen at the base of the left and right coronary or triangu-
fuchsin
them
the
In 7.3 % of cases necrotic
in
along
old hemorrhages
stain.
of
crystals
These
produce
stone
2 and
in 7.2) sect-
found
Most
black
large
while
were
(Fig, more
for more than
compressed
in the
or
than 13 weeks, though the grade of deposition varied in each case (Fig. 4).
trichrome,
calcium
: Gallstones
cases
fine
Kossa's
cubic
a large inclusion body
nce at the periphery
frequently
fixed
carbol
with
ned lipof uscin
after
(pH
Masson's
elongated
these
hepatocytes
occurred
tested
In hepatocytes
hematoxylin-eosin,
f,
Nuclei having
substances.
each
carotid
were
Verhoeff's
in animals
in
examined
bodies
weeks.
around
bodies,
found
uently
of
Five ƒÊcm
with
2
25mg/kg
formalin
52.6%
in the nuclear
collected
paraffin.
stained
Prussian
of from
they
of cases
with
dose
were
buffered
embedded
ions
a
from
various
anesthetized
organs
neutral
and
termination
thoroughly
10
or
the were
hyaline were
inclusion
for to
in or
rectangular,
were
in
automatic
served
pentobarbital
gross
housed
acclimatization
After
Main
lighting
hepatocytes
present
Such inclusion
conditioned
and
degenerated
granulomas. Intranuclear
given
and
A few
occasionally
age,
animals
with
were
exanguinated
%
rooms
cages
they
artery.
were
of
over
7 : 30pm,
they
experiment,
and
(Fig,
2).
parben-
humidity
to
weeks,
sodium
the liver of 59.9% of cases examined
capsule
were
or
test
alminium
experiments.
to
age
to
supply.
4
parben-
30mg/kg
weeks
containing
of
7 : 30am
or
of 4
Tokyo)
55•}1%
individual water
or
month.
months
24•}1•Ž,
from
and
CLEA,
transferred
at
syrup
dose
2
biscuits
each At
in
at
(Medical,
during
control in
daily
days
thereafter
dazole
parasite
Tokyo)
of
They
were 2 to l0pm in diam-
163
eter
without
halo
and
were
positive
for
periodic acid Schif f and Verhoef f's carbol fuchsin acid-fast stain (Fig. 5). Bilateral epithelial
hyperplasia cells
was
of the
seen
follicular
in the
and light
nucleus
abundant
cytoplam
iferation
and
tissues
and
showed
abnormally
a nodular
compressed
was also seen
frequently
prol-
appeared
small
or brown
to millet grains most
in the but
they
being
caudal
thickened increase
parts
and diffuse
surface
not extensignificantly showed
small
deposit
an
foci
of brown
(Fig. 8). also seen
Occasionally, calcification along collagen fibers.
incidences
of these
ected dams
according or sires
which had experiments, Cysts found
to types from
the
were
was While
not
aff-
histiocytes
appeared
(Fig. 10).
or
their
debris
encountered
in
eggs of the parasite examinations.
lobe of the
hypoph-
lesions
Fragments
were
of
frequently
lesions,
while
were detected
: Many
investigations
on beagles
; breeding
no
in fecal have systems
[3] as well as hematology
[1, 2,
5, 10] and biochemistry [4, 7, 9]. Pathological backgrounds, however, remain unclear despite of the importance in reading effects
of
given
they
experimental
the results
treatment.
presented
here
could
to all tests using beagles,
would provide
some
informations
to
pathologist engaged in the subject. In the present study there were many kinds
of
changes
them gallstones
cells of the anterior
idered
a small
round
of connective
studied
ysis from 35.1 % of cases examined. The wall of these cysts consisted of flattened lobe containing
small
Discussion
breeding
were
to be
granulomatous
tissues
not be applicable
20 to 200 dim in diameter
and
these
of
the lung In other
small
by a thin layer
Although
colony,
too
sizes
revealed that these by a number of
larvae
and diets
lymin the to be
having
were
Histology infiltrated
encapsulated
been
liver,
mesenteric
nodules
lesions
seen grossly. lesions were
of experiments,
not been subjected to any rarely exhibited such changes.
in the anterior
the
in 33.1
lungs,
others. The lesions and spleen appeared
or brown
organs
of
pigments, and a number of macrophages were seen to phagocytize the pigments
changes
pancreas,
while of Tox-
detected
in the
cells, and sometimes 2
only within
of the capsule with
spleen,
eosinophils,
periphery cut
usually
of collagen
hemorrhage
poppy
attaining
Microscopically,
were
millet grains to soy beans and lesions accompanied atelectasis.
On the
the capsule
ding deeply.
males,
like
were seen to be localized
or beneath
larvae
examined,
kidneys,
of
bodies
sometimes
or 3cm long (Fig. 7).
13.7 %
remained unclear. caused by migration
canis
cases
was seen in the
white
in size were found grossly
frequently
of the capsule,
nodules
from
amorphous
was
Gandy-Gamna bodies in the splenic capsule of 43.1 % of cases examined. Usually, a few white
inflammation
pathogenesis Damages ocara
basophilic
mitosis
rare. There
(Fig. 9).
Chronic
phnodes and liver, kidneys
hyperplasia
but
materials
of
neighbouring
(Fig. 6). Adenomatous
of weakly
prostates
thyroid
glands from 46.3 % of cases examined. Hyperplastic cells having a larger and light nucleus
amount
damage.
in the
liver.
Among
seemed not to cause serious
However,
granulomas
to be of importance
were
cons-
in evaluating
164
effects
of
such with of
experimental
lesions
treatments,
were
degeneration
and
hepatocytes Intranuclear
scin more
of
more
than
were
known
to
of
dogs
process
bodies
in
13
study
might
during
experiments.
appear
cells
asmic
that
in
stress.
The
which
after
parasitic study
to
experiments
as suggested
after
as gestatliving body
in
might
stimulation lesions
might
initiated
during
mechanism
present
larvae of the
matured
present
eggs
such
that parts
be
in the
stresses
aging
the
to
the
T oxocara stress
suggested or other
by larvae
changes
by
obse-
be induced migrate
by
through by
a
Johnson.
stresses
found
correspond
in
et
li-
[1]
to •gintracytopl-
nonglycogenic al
inclusion
lysosomal
rved
of
some
References
Murti
such
and
migrate by
for
dogs,
inclusions
might
by
aged
accelerated
PAS-positive
described
these
in
u-
seen
tested
examined be
lipof
were
Since
Intracytoplasmic ver
and
animals
weeks.
excretion
exposed
muscles
hepatocytes
frequently
ion,
necrosis
them.
hyaline
deposition
cell
for
being
associated
single
around
tive
since
sometimes
[8].
globule•h who
alterations
of
21.
suggested
bodies
Andersen, A. C. and Gee, W. (1957). Blood values in the beagle. Veterinarian, 18, 16-
[2]
represented
hepatocytes
due
Andersen, A. C. and Gee, W. (1958). Normal blood values in the beagle. Vet. Med., 53 135-139.
to
nonspecific
relation
slight
to
the
cases the
thyroid
that
examined
as
in
lobe
35.1%
enital
follicular
were
we
could
and
of
assumed
sires
of
the
Prostatitis with
some
urine
tests.
see
fluctuation
in
occurbe
[4]
cong-
was
no
changes.
bodies 43.1
%
to
be
of
such
in
cases
[6]
with
in
dams
[7]
colony. to
be
of
Iowa,
USA.
correlated
parameters
T oxocara of
cases
ication [6]
canis
larvae
suggests of
described
this
much parasitic that
some
by detected
dogs
of
in
33.1% of
infection.
R.H. and Trum, B. F. (1964). of dogs for biochemical reseaCare, 14, 226-234.
age. J. Gerontol.,
Johnson,
20, 131-138.
R.M., Andersen,
A.D. and Gee, W.
Parasitism in an established dog Lab. Animal Care, 13, 731-736.
McKelvie,
D.H., Powers,
S. and McKim, F. for on
posi-
Amer. J, vet. Res., 27, 1405-1512.
Murti, G.S. and Borgmann, R. (1965). Intrahepatic periodic acid-Schiff-positive nonglycogenic
erad-
Their
Johnson became
Press,
in
migration
difficulty
A. C.,
University
(1966). Microanalytical procedures blood chemistry long-term study
[8] caused
Dog, Andersen, State
Dougherty, J.H. and Resenblatt, L.S. (1965). Changes in the hemogram of the beagle
beagles. Inflammation
Iowa
Charlewood, Acquisition
(1963). kennel.
since
cases
The
with
exa-
related
editor,
rch. Lab. Animal [5]
observed
experiments,
breeding seemed
cysts
there two
during
rarely
of
to
Andersen, A. C. and Goldman, M. (1970). Growth and development. In The Beagle as an Experimental
46.3%
hypophysis
the
capsule
cells
small
whereas
like
handlings
in
considered
Gandy-Gamna
forced
as
the
were
mined
seen
well
between
splenic
epithelial
were
of
defects,
correlation
the
in
[3]
of
anterior
ring
caused
hepatotoxicity.
Hyperplasia of
damage
globules
histochemical
in
canine
characterization.
liver: Amer.
J. vet. Res., 26, 63-67. [9]
Nagode,
L. A., Walter,
J. F. and walter,
F.
165
L. (1966). Enzymatic tissues. [10]
activities
of canine
Soave, O.
A. and
Boyle,
ned
C. C. (1965).
A
Explanation Fig.
1
Fine
crystaline
old Fig.
2
gallstones
of
of
lipof of
uscin
stain. •~
few
incluison
Nodular
Fig.
a and
hepat-
stain. •~ deposit
in
7
Fig.
8
mar-
Fig.
9
acid-
of
hepatocytes bodies
hepatocytes.
(arrowere
26-month-old Schiff
reaction. •~
10
thyroid
body
epit-
gland.
28sta-
figure
Photo.
7.
of
fibers
the
nodules
shown increased
with
cyst
abundant
of
hemo-
Prussian
the
female,
blue
hypophysis.
26-
hematoxylin-eosin
100.
larvae
in. •~
the
(arrows).
Inflammatory
fem-
in male.
100.
Congenital
month-old
nodules
Significantly
deposits
seen
like
16-month-old
Microscopic
nis
follicular
hematoxylin-eosin
capsule.
month-old
Fig.
the
Gandy-Gamna
stain. •~ intracytoplasmic
inclusion
of
reaction. •~ fe-
f uchsin
dogs.
200.
siderin
cytop-
of
male,
collagen
26-month-old carbol
bodies
periodic-acid
cells
in
1000.
the
conditio-
15, 359-362.
hyperplasia
in. •~
25-month-old
globular
Two some
in
Care,
month-old
400.
and
ws).
body nucleus
hepatocytes.
Verhoeff's
Large
ale,
a
lymphocytes.
chromatins.
Diffuse
fast
with by
enlarged
hamatoxylin-eosin
in
6
splenic
female,
lasm
5
Fig.
hematoxylin-eosin
inclusion with
gination
Fig.
liver
female,
Intranuclear
male,
Animal
of
laboratory
Figures
400.
ocyte
4
the
surrounded
stain. •~
Fig.
18-month-
hemograms
non-conditioned
helial
Granuloma
13-month-old
3
.
of
of the
and
Lab.
male.
histiocytes
Fig.
comparison
Amer. J. vet. Res., 27, 1385-1393.
reactions in
cortex
male,
of
to
T oxocara
the
kidney.
ca18-
hematoxylin-eosin
sta-
200.
400.
毒 性 試 験 に 用 い られ た ビ ー グ ル 犬 の 偶 発 病 変 真 板 敬 三 ・増 田
裕 ・鈴 木 善 雄
三共株式会社安全性試験センター 1才 か ら4才 の ビー グル犬420頭
について偶発病変を
甲状 腺 で 小胞 上 皮 細 胞 の 過 形 成 が46.3%に,ま
検 索 した と ころ,肝 に お い て胆 石 が87%,内
芽 腫 が59.9
膜 のGandy-Gamna氏
%,肝
ポ フス チ ン
体 前 葉 に おけ る小 の う腫 が35.1%見
細 胞 の硝 子 様 核 内封 入 体 が52.6%,リ
沈 着 が50%,好 ま た,靱
酸 性 細 胞 質 内 封 入 体 が3.7%に
帯 付 着 部 の実 質 壊 死 が3.7%に
認 め られ,
み られ た 。肝 細
胞 の硝 子 様 核 内 封 入 体 お よび リポ フス チ ン沈 着 は,実 験 期 間が13週 間 以 上 の場 合 高 率 に 認 め られ た 。肝 以 外 では,
た,脾 被
小 体様 の 結 節 が43.1%に,下 出 され,さ
垂
らに 雄 の
13.7%に 前 立 腺 炎 が 認 め られ た 。 ま た,犬 回 虫 の幼 虫が 体 内移 行 す る際 に 生 じた 病 変が,33.1%の 肺 等 に 認 め られ た 。
犬 の肝 ・腎 ・
166
167
