HHS Public Access Author manuscript Author Manuscript
ACS Chem Biol. Author manuscript; available in PMC 2017 April 15. Published in final edited form as: ACS Chem Biol. 2016 April 15; 11(4): 992–1000. doi:10.1021/acschembio.5b00806.
Stable Colloidal Drug Aggregates Catch and Release Active Enzymes Christopher K. McLaughlina,b, Da Duanc, Ahil N. Ganesha,b, Hayarpi Torosyanc, Brian K. Shoichetc, and Molly S. Shoicheta,b Brian K. Shoichet: [email protected]; Molly S. Shoichet: [email protected] aDepartment
Author Manuscript
of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, Canada M5S 3E5 bInstitute
of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9 cDepartment
of Pharmaceutical Chemistry, University of California, San Francisco, 1700 Fourth Street, San Francisco, California 94158-2550, United States
Abstract
Author Manuscript
Small molecule aggregates are considered nuisance compounds in drug discovery, but their unusual properties as colloids could be exploited to form stable vehicles to preserve protein activity. We investigated the co-aggregation of seven molecules chosen because they had been previously intensely studied as colloidal aggregators, co-formulating them with bis-azo dyes. The co-formulation reduced colloid sizes to
ACS Chem Biol. Author manuscript; available in PMC 2017 April 15. Published in final edited form as: ACS Chem Biol. 2016 April 15; 11(4): 992–1000. doi:10.1021/acschembio.5b00806.
Stable Colloidal Drug Aggregates Catch and Release Active Enzymes Christopher K. McLaughlina,b, Da Duanc, Ahil N. Ganesha,b, Hayarpi Torosyanc, Brian K. Shoichetc, and Molly S. Shoicheta,b Brian K. Shoichet: [email protected]; Molly S. Shoichet: [email protected] aDepartment
Author Manuscript
of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, Canada M5S 3E5 bInstitute
of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, Canada M5S 3G9 cDepartment
of Pharmaceutical Chemistry, University of California, San Francisco, 1700 Fourth Street, San Francisco, California 94158-2550, United States
Abstract
Author Manuscript
Small molecule aggregates are considered nuisance compounds in drug discovery, but their unusual properties as colloids could be exploited to form stable vehicles to preserve protein activity. We investigated the co-aggregation of seven molecules chosen because they had been previously intensely studied as colloidal aggregators, co-formulating them with bis-azo dyes. The co-formulation reduced colloid sizes to
