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OPEN
received: 13 October 2016 accepted: 23 February 2017 Published: 27 March 2017
Stepwise analysis of MIR9 loci identifies miR-9-5p to be involved in Oestrogen regulated pathways in breast cancer patients Raffaela Barbano1,*, Barbara Pasculli1,*, Michelina Rendina1, Andrea Fontana2, Caterina Fusilli3, Massimiliano Copetti2, Stefano Castellana3, Vanna Maria Valori4, Maria Morritti4, Paolo Graziano5, Ciuffreda Luigi6, Michelina Coco1, Francesco Picardo7, Tommaso Mazza3, Ella Evron8, Roberto Murgo6, Evaristo Maiello4, Manel Esteller9,10,11, Vito Michele Fazio1,7 & Paola Parrella1 miR-9 was initially identified as an epigenetically regulated miRNA in tumours, but inconsistent findings have been reported so far. We analysed the expression of miR-9-5p, miR-9-3p, pri-miRs and MIR9 promoters methylation status in 131 breast cancer cases and 12 normal breast tissues (NBTs). The expression of both mature miRs was increased in tumours as compared to NBTs (P
OPEN
received: 13 October 2016 accepted: 23 February 2017 Published: 27 March 2017
Stepwise analysis of MIR9 loci identifies miR-9-5p to be involved in Oestrogen regulated pathways in breast cancer patients Raffaela Barbano1,*, Barbara Pasculli1,*, Michelina Rendina1, Andrea Fontana2, Caterina Fusilli3, Massimiliano Copetti2, Stefano Castellana3, Vanna Maria Valori4, Maria Morritti4, Paolo Graziano5, Ciuffreda Luigi6, Michelina Coco1, Francesco Picardo7, Tommaso Mazza3, Ella Evron8, Roberto Murgo6, Evaristo Maiello4, Manel Esteller9,10,11, Vito Michele Fazio1,7 & Paola Parrella1 miR-9 was initially identified as an epigenetically regulated miRNA in tumours, but inconsistent findings have been reported so far. We analysed the expression of miR-9-5p, miR-9-3p, pri-miRs and MIR9 promoters methylation status in 131 breast cancer cases and 12 normal breast tissues (NBTs). The expression of both mature miRs was increased in tumours as compared to NBTs (P
