This article was downloaded by: [University of Pennsylvania] On: 25 January 2015, At: 22:10 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Studies on avian infectious bronchitis virus in New Zealand I. Serotypes & J.E. Lohr Dr.Vet.Med.
a b
a
Department of Veterinary Pathology and Public Health , Massey University , Palmerston North b
Hiltl Str 27, D8035, Gauting, W. Germany Published online: 23 Feb 2011.
To cite this article: I. Serotypes & J.E. Lohr Dr.Vet.Med. (1977) Studies on avian infectious bronchitis virus in New Zealand, New Zealand Veterinary Journal, 25:3, 48-51, DOI: 10.1080/00480169.1977.34356 To link to this article: http://dx.doi.org/10.1080/00480169.1977.34356
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
48
NEW ZEALAND VETERINARY JOURNAL
VOL. 25
Studies on avian infectious bronchitis virus in New Zealand I. Serotypes, J. E. Lohr* N.z.
vel.
J.
25: 48-51
SUMMARY
Downloaded by [University of Pennsylvania] at 22:10 25 January 2015
Twelve New Zealand isolates of avian Infectious Bronchitis virus were typed by the serum-neutralization test. Both the constant serum decreasing virus and the constant virus decreasing serum methods were used. Cross-protection tests in specific-pathogen-free chickens were also carried out. Four groups of viruses, designated serotypes A, B, C and D were distinguished by both methods. Immunization with B, C and D gave some protection to challenge with type A.
INTRODUCTION
Antigenic variation exists among infectious bronchitis (IB) virus (IS), and notifications have included the well-known U.S. strains (14). the Australian T strain (25) (5) (6), a New Zealand strain (6). a German field isolate (5), and several Italian strains (27). IB virus isolates from many other countries have been classified either as Massachusetts or Connecticut types (3) (9) ( 10) (6) (20) (24) ( II) or classification was impossible (8) or not attempted. No entirely satisfactory Isystem for classifying IB virus isolates has yet been devised (i 2) but it has been suggested that the classification of avian IB viruses be approached in a similar way to the reciprocal serum-neutralization method used in the classification of human rhinoviruses (7) as reported by Kapikian et al. (16). A serum neutralization test (SNT) may be performed by two methods. either by decreasing virus constant serum (alpha) or by the constant virus decreasing serum (beta) method. Some of the important factors of the serum-neutralization test, particularly with regard to I B. were discussed by Page and Cunningham (21) and v.Bulow (4). According to these authors the results of the tests by the alpha method depend on the slope of the neutralization line. but the slope of the neutralization line is irrelevant for the beta method, because this method reflects differences in antibody concentration (21). The following reports an attempt to serotype 12 isolates of avian IB virus by the alpha alJd beta methods of the SNT in embryonated eggs and a limited number of cross-protection tests using specific pathogen-free chic.\l6.0 3.8 nd >4.0 nd nd 0.7 < 1.0
New Zealand Veterinary Journal Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/tnzv20
Studies on avian infectious bronchitis virus in New Zealand I. Serotypes & J.E. Lohr Dr.Vet.Med.
a b
a
Department of Veterinary Pathology and Public Health , Massey University , Palmerston North b
Hiltl Str 27, D8035, Gauting, W. Germany Published online: 23 Feb 2011.
To cite this article: I. Serotypes & J.E. Lohr Dr.Vet.Med. (1977) Studies on avian infectious bronchitis virus in New Zealand, New Zealand Veterinary Journal, 25:3, 48-51, DOI: 10.1080/00480169.1977.34356 To link to this article: http://dx.doi.org/10.1080/00480169.1977.34356
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http:// www.tandfonline.com/page/terms-and-conditions
48
NEW ZEALAND VETERINARY JOURNAL
VOL. 25
Studies on avian infectious bronchitis virus in New Zealand I. Serotypes, J. E. Lohr* N.z.
vel.
J.
25: 48-51
SUMMARY
Downloaded by [University of Pennsylvania] at 22:10 25 January 2015
Twelve New Zealand isolates of avian Infectious Bronchitis virus were typed by the serum-neutralization test. Both the constant serum decreasing virus and the constant virus decreasing serum methods were used. Cross-protection tests in specific-pathogen-free chickens were also carried out. Four groups of viruses, designated serotypes A, B, C and D were distinguished by both methods. Immunization with B, C and D gave some protection to challenge with type A.
INTRODUCTION
Antigenic variation exists among infectious bronchitis (IB) virus (IS), and notifications have included the well-known U.S. strains (14). the Australian T strain (25) (5) (6), a New Zealand strain (6). a German field isolate (5), and several Italian strains (27). IB virus isolates from many other countries have been classified either as Massachusetts or Connecticut types (3) (9) ( 10) (6) (20) (24) ( II) or classification was impossible (8) or not attempted. No entirely satisfactory Isystem for classifying IB virus isolates has yet been devised (i 2) but it has been suggested that the classification of avian IB viruses be approached in a similar way to the reciprocal serum-neutralization method used in the classification of human rhinoviruses (7) as reported by Kapikian et al. (16). A serum neutralization test (SNT) may be performed by two methods. either by decreasing virus constant serum (alpha) or by the constant virus decreasing serum (beta) method. Some of the important factors of the serum-neutralization test, particularly with regard to I B. were discussed by Page and Cunningham (21) and v.Bulow (4). According to these authors the results of the tests by the alpha method depend on the slope of the neutralization line. but the slope of the neutralization line is irrelevant for the beta method, because this method reflects differences in antibody concentration (21). The following reports an attempt to serotype 12 isolates of avian IB virus by the alpha alJd beta methods of the SNT in embryonated eggs and a limited number of cross-protection tests using specific pathogen-free chic.\l6.0 3.8 nd >4.0 nd nd 0.7 < 1.0
