Technical notes 9. AMONENKO V. M., KRUGLYKIt A. A., PAVLOV V. S. and TIKHINSKII G. F. Izv. Akad. Nauk SSSR. Metal. 1, 190 (1966). 10. KRUGLYKHA. A., PAVLOVV. S. and TIKHINSKII G. F. Zh. Neorgan. Khim. 10, 285 (1965).
International Journal of Applied Radiation and Isotopes, 1975, Vol. 26 pp. 567-569. Pergamon Press. Printed in Northern Ireland
567
The autoradiographs were prepared using stripping film (Kodak A R 10) according to technique of ROORRSIs). The grains were counted using an oil immersion objective and an eye piece graticule. The microscope was always focused on the level from which the maximum grain count was obtained. The grains under 5 preselected squares were counted from each focus.
Results Studies on the Incorporation of Carbon-14 i n T i s s u e s o f Galleria meUoneUa (Received 26 July 1974; and in revisedform 25 November 1974)
Introduction IN THE study of the effect of ~4C on insects, specially on oogenesis and egg development one of the problems encountered is to identify a suitable carrier of 14C that can be used as an internal source of betaradiation. From that point of view the study of general distribution of 14C in different tissues of insects needs special attention. Objective of this study is to introduce 14C through different carriers separately in Galleria mellonella in one stage of its development and to observe and compare the pattern of distribution of a4C in the key tissues of the insect.
The radioactivity observed in different tissues 15 days after feeding is shown in Fig. 1. Radioactivity in the cuticle and in the wing was very low, compared with that of other tissues. Overall, low radioactivity was observed in the glycine-14C group. The radioactivity in the fatbody and in the unlaid eggs of insects fed glycerol-14C was greater than for insects fed glucose-a4C or glycine-14C. In the laid eggs of the glycerol-14C group the activity was only slightly lower than for the unlaid eggs. However, radioactivity in the lald eggs of the glucose-14C and glycine-x4C groups was 47 and 54 per cent higher than in unlaid eggs; these differences were statistically significant (P values for glucose and glycine groups were
International Journal of Applied Radiation and Isotopes, 1975, Vol. 26 pp. 567-569. Pergamon Press. Printed in Northern Ireland
567
The autoradiographs were prepared using stripping film (Kodak A R 10) according to technique of ROORRSIs). The grains were counted using an oil immersion objective and an eye piece graticule. The microscope was always focused on the level from which the maximum grain count was obtained. The grains under 5 preselected squares were counted from each focus.
Results Studies on the Incorporation of Carbon-14 i n T i s s u e s o f Galleria meUoneUa (Received 26 July 1974; and in revisedform 25 November 1974)
Introduction IN THE study of the effect of ~4C on insects, specially on oogenesis and egg development one of the problems encountered is to identify a suitable carrier of 14C that can be used as an internal source of betaradiation. From that point of view the study of general distribution of 14C in different tissues of insects needs special attention. Objective of this study is to introduce 14C through different carriers separately in Galleria mellonella in one stage of its development and to observe and compare the pattern of distribution of a4C in the key tissues of the insect.
The radioactivity observed in different tissues 15 days after feeding is shown in Fig. 1. Radioactivity in the cuticle and in the wing was very low, compared with that of other tissues. Overall, low radioactivity was observed in the glycine-14C group. The radioactivity in the fatbody and in the unlaid eggs of insects fed glycerol-14C was greater than for insects fed glucose-a4C or glycine-14C. In the laid eggs of the glycerol-14C group the activity was only slightly lower than for the unlaid eggs. However, radioactivity in the lald eggs of the glucose-14C and glycine-x4C groups was 47 and 54 per cent higher than in unlaid eggs; these differences were statistically significant (P values for glucose and glycine groups were
