Biol Trace Elem Res DOI 10.1007/s12011-015-0269-8
Study on Biological Effects of La3+ on Rat Liver Mitochondria by Microcalorimetric and Spectroscopic Methods Man Wu & Jia-Ling Gao & Zhi-Jiang Feng & Wen Liu & Ye-Zhong Zhang & Yi Liu & Jie Dai
Received: 24 January 2015 / Accepted: 10 February 2015 # Springer Science+Business Media New York 2015
Abstract The effects of lanthanum on heat production of mitochondria isolated from Wistar rat liver were investigated with microcalorimetry; simultaneously, the effects on mitochondrial swelling and membrane potential (Δψ) were determined by spectroscopic methods. La3+ showed only inhibitory action on mitochondrial energy turnover with IC50 being 55.8 μmol L−1. In the spectroscopic experiments, La3+, like Ca2+, induced rat liver mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor, cyclosporine A (CsA). The induction ability of La3+ was stronger than that of Ca2+. These results demonstrated that La3+ had some biotoxicity effect on mitochondria; the effects of La3+ and Ca2+ on rat liver mitochondrial membrane permeability transition (MPT) are different, and La represents toxic action rather than Ca analogy. Keywords Mitochondria . Metabolism . La3+ . Permeability transition
Introduction With widespread applications, large amounts of rare earth elements (REEs) have been entered into the environment through various channels during the past few decades. Their ecotoxicological effects and physiological mechanism on M. Wu : J.
Study on Biological Effects of La3+ on Rat Liver Mitochondria by Microcalorimetric and Spectroscopic Methods Man Wu & Jia-Ling Gao & Zhi-Jiang Feng & Wen Liu & Ye-Zhong Zhang & Yi Liu & Jie Dai
Received: 24 January 2015 / Accepted: 10 February 2015 # Springer Science+Business Media New York 2015
Abstract The effects of lanthanum on heat production of mitochondria isolated from Wistar rat liver were investigated with microcalorimetry; simultaneously, the effects on mitochondrial swelling and membrane potential (Δψ) were determined by spectroscopic methods. La3+ showed only inhibitory action on mitochondrial energy turnover with IC50 being 55.8 μmol L−1. In the spectroscopic experiments, La3+, like Ca2+, induced rat liver mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor, cyclosporine A (CsA). The induction ability of La3+ was stronger than that of Ca2+. These results demonstrated that La3+ had some biotoxicity effect on mitochondria; the effects of La3+ and Ca2+ on rat liver mitochondrial membrane permeability transition (MPT) are different, and La represents toxic action rather than Ca analogy. Keywords Mitochondria . Metabolism . La3+ . Permeability transition
Introduction With widespread applications, large amounts of rare earth elements (REEs) have been entered into the environment through various channels during the past few decades. Their ecotoxicological effects and physiological mechanism on M. Wu : J.
