ARCHIVES

OF BIOCHEMISTRY

Department

AND

BIOPHYSICS

166, 651-682 (19%)

Subunit

Constitution

DENNlS

W. DARNALL

of Proteins: AND

IRVING

A Table’ M. KLOTZ

0,’ Chemistry, New Mexico State University, Las Cruces, New Mexico Division, Department of Chemistry, Northwestern University, Evanston, Received September

The wide response from readers to our previously published protein subunit tables (l-4) indicates the usefulness of such compilations for teaching and research purposes. We have prepared, therefore, a new updated listing of proteins with subunits held together by noncovalent bonds (Table I). Individual polypeptide chains held together by disulfide bridges have not been individually classified as subunits; insulin, for example, is listed as having a subunit molecular weight of 5733 even though this is the combined weight of the disulfide-linked A and B chains. Kleine (4a) has compiled a list of proteins whose subunits are associated through both noncovalent and covalent (disulfide) bonds. In many instances the subunit listed (Table I) ma,y not be the minimal subunit obtainable, but instead the minimal subunit that has been unequivocally obtained under conditions that eliminate cleavage of

88003 and Biochemistry Illinois 60201

9, 1974

peptide or disulfide bonds. For some proteins two or more stages of dissociation can be clearly recognized; in such instances two or more entries specifying the relations between the different aggregates are given. Parentheses around molecular weights or subunit numbers indicate uncertainty in the value. The most accessible references are given for each entry; they do not necessarily indicate the source most deserving of credit for establishing the subunit stoichiometry. These sources are mentioned in the cited works. Since Table I contains well over 500 entries, it seemed appropriate to make an alphabetical listing of the entries in Table I. This listing is found in Table II; the numbers in parentheses in Table II indicate the position at which the protein will be found in Table I.

‘Preparation of this Table was assisted by support made available by Research Grants from the National Science Foundation (GB-35206) and from the National Institutes of Health (GM-32014). 651 Copyright @ 1975by Academic Press, Inc. *I,

rights

nf renmrin,mtin-

cm n-..

p-----

652

DARNALL

AND KLOTZ

TABLE SUBUNIT

Protein

Insulin (5) S-100 protein (6)

SOWX

Bovine

Mercaptopyruvate sulfur transferase (EC 2.8.1.2) (7) As .,&Oxosteroid isomerase (EC 5.3.3.1) (8) Nerve growth factor (9) Luteinizing hormone (10)

OF PROTEINS

T

Organ

Brain

Pseudomonas

Mouse Obine

Cytochrome (CC’) (11) Luteinizing hormone (12) Ribonuclease (13) Interstitial cell-stimulating hormone (14)

I

CONSTITUTION

Bull 1Ovine

Phospholipase A, (14a) Luteinizing hormone (15) Superoxide dismutase (16) Superoxide dismutase (17) Thyrotropin (17a)

Submaxillary gland

1

Molecular weight No.

MoleUJlar weight

11,466 19.500

(4:

23.800

2

5.733 (4.1(07,000) 12,000

26,300

2

13,000

26.518 27.322

2 1 1 2 1 1 2 1 1 2 2 2 2 1 1 4

1x2.59 12.%500 14.830 14,000 l&F53 14,407 14,000 13.700 16.300 15,000 15.500 16,800 16.000 1,5,000 15,000 8.000 16,500 16.000 11.000

Pituitary gland

28.000 28,260

Semen Pituitary gland

29.000 30,000

Venom Pituitary gland

30,000 31.000 31,000 32,000 32.000

Erythrocyte

Subunits

Lactose-specific factor III (18)

E. co/r and Staphylococcus

Follicle-stimulating

Equine

Pituitary gland

33,800

Human

Erythrocyte

34,000

1 1 3

Erythmcyte Pituitary gland Milk

34,000 35,000 35.000 35.000

2 2 2 3

17.500 17.L500 Ii.500 12.000

Germ

Halobacterium

35.000 36.000

&Hydroxydecanoly thioester dehydrase (27) Rhodanese (EC 2.8.1.1) (28) Chorionic gonadotropin (29)

E. cob

36,000

2 1 1 2

17,000 18,000 18,000 18,000

Chymotrypsin inhibitor I (30) Superoxide dismutase (31) Hemerythrin (31a) Proteinase inhibitor I(321

potato E. coli Phascolosoma

Dethiobiotin synthetase (32a) Dihydropteridine reductase (33) Nucleoside phosphotransferase (34)

E. coii Sheep Carrot

Biotin carboxyl carrier protein (35) Catabolite gene-activator protein (36) Cytoplasmic protein (37) Growth hormone (38) Factor X (39)

E. coli E. co/i Neurospora

Hemagglutinin LcH (39a) Phycocyanin (40)

Lens Chroomonas

Phenylalanine hydroxylase-stimulating protein (41) Galactokinase (EC 2.7.1.6) (42) Triosephosphate isomerase (EC 5.3.1.1) (43) -

Rat

Liver

51,.50(1

2 1 1 4 2 3 2 2 2 2 1 1 2 2 3 2 1 1 2 2 2 4

18,500 14,900 23,000 9,800 21,600 12.700 19.:100 9,400 24.500 21.000 22.000 22,000 22.500 22.ono 1R,000 2s.000 20.000 30,000 24,500 16,000 10,~X)O 12.500

Human

Erythrocyte

Rabbit

MUSCk

53.000 53,000

2 2

27.000 26,500

hormone (19)

Adenine phosphoribosyltransferase (EC 2.4.2.7) (20) Hemoglobin I(211 Follicle-stimulating hormone (22) &Lactoglobulin (23) Lactose-specific phosphocarrier protein (24) Agglutinin (25) DNA-dependent RNA polymerase (26)

Blood clam 1Human i Bovine Staphylococcus

Wheat

33,000

Liver

Coelomic fluid

Potato

Bovine Bovine

Liver

Pituitary gland Plasma

37,000 37,900 39,000 39..ioo 40.600 42,000 19.400 42,000 42,000 44.000 45,000 45,000 45,000 48,000 48.000 49.000 50,000

SUBUNIT

CONSTITUTION TABLE

Protein

653

OF PROTEINS

I-Continued

Source

Molecular weight

T

Subunits NO.

Molecular weight

54,Ocil 54,OOa 55,000 55,000 55,000

4 2 2 4 2

13,500 27,000 27,500 14,000 30,coo

Malate dehydrogenase (EC 1.1.1.37) (44) Transglutaminasa (45) Azoferredoxin (46) Hyaluronidase (EC 3.2.1.:15) (47) 5.lO-Methglenetetrahydn,folate dehgdrogenase (EC 1.5.1.5) (48) w-Amidase (49) Alcohol dehydrogenase (EC 1.1.1 .l) (49a) NADP-Linked isocitrate dehydrogenase (EC 1.1.1.42) (50) Deoxycytidylate deaminase (51 I Hydrogenase (EC 1.12.1.1l (52) Lactose synthetase ,531

Rat Drosophila

Live1

58,CilO 60,000

2 8

27,000 7,400

Porcine

Heart

60,000

2

32,000

Staphylococcus Clostridium Bovine

Milk

60,OOQ 60,000 60,000

Transcohalamin

Human

Plasma

60,000

Liver

60,000

2 2 1 1 1 1 2

29,000 30,000 22,300 36,600 38,000 25,000 29,000

1 1 4 1 1 4 2 2

22.300 36,600 15,500 31,000 31,000 16,000 34.000 31,500 16,000 41,500 28,500 31,000

I[ (,541

Neurospora Guinea pig Clostridium Bovine Clostridium

Organ

Hair follicle Testicle

Nuclear DNA polymerase (EC 2.7.7.7) (55) Aldose reductase (EC 1.1.1.211 (56)

Rhodotorula

Prealhumin (57) Phosphoglucomutase

Human Rabhit

Plasma Muscle

62,000 62,000

Erythrocuprein (59) Serinedehydratase (EC 4.2.1.131 (60) T2 Phage-induced thymidylatesynthetase 161) Hemoglobin (62) T,- T, Complex (63)

B0VilW Rat Lactobacillus

Blood Liver

64,000 64,000 64,400

Mammalian E. colr

Erythrocytes

64,500 65,000

L-3.Hydroxvacyl-C’r)A dehydrogenase (EC 1.1.1.351 (64) Inorganic pyrophcxphatase (EC 3.6.1 .l) (El,1 Thiogalactoside transacetylase (EC 2.3.1.18) (66) Phosphoglycerate mutase (EC 2.7.5.3) (67) Thioredoxin reductase (67a) Malate dehydrogenase (EC 1.1.1.37) (68) Malate dehydrogenase (EC 1.1.1.37) (69) Glucokinase (EC 2 7.1.12) (70) O-Acetylserine sulfhydrylase A (711 Tropomyosin B (72) Transaldolase III (EC 2.2.1.2) (73) Adenylate kinase (EC 2.7.4.X) (74) Glycerol-:i-phosphate dehydrogenase (EC 1.1.1.8) (75) lT@-Estradiol dehydrogenase (76) Avidin (77) Hemoglobin III ($8: D-Glycerate dehydrogenase (78a) o-Lactatedehydrogenase IEC 1.1.1.28) (79) Fructose diphosphate aldolase (EC4.1.2.13) (80: Malate-lactate transhydrogenase ~. (EC 1.1.99.7) (811 Thymidylate synthetase (EC 2.1.1.6) (82) Hydroxypyruvate reductase (EC 1.1.1.29) (831 Nucleoside diphosphate kinase (EC 2.7.4.6) (841 NAD-glycohydrolase (EC 3.2.2.5) (85)

Pig

Heart

65,ooO

4 1 1 2

Yeast

65,000

2

32,000

1E. coli

65,300

2

29,700

66,000

2

33,000

Liver Muscle, thorax

66,000 66,300 67,000 67,000 68,000 68,000 68,000 68,000 68,000

2 2 2 2 2 2 2 3 2

32,000 37,500 35,000 34,500 34,000 33,500 34,000 23,000 34,000

Placenta Egg white Erythrocyte Liver Muscle

68,000 68,300 69,000 70,000 70,000

2 4 4 2 2

33,000 18,000 17,500 34,000 35,000

2

35,000

2

35,000

2 2

35,000 35,000

Seed

4

17,000

Liver

2

38,000

(EC 2.7.5.a) (58)

61,000

Pig

Muscle

E. coli Rat Pig Bacillus

Liver Heart

Salmonella Rabbit Candida Rat Chicken, Rabbit, Honeybee Human Chicken Blood Clam Beef Limulus

Muscle

E. co11

Lactobacillus Pseudomonas

Iz,,e,,,,,

rahbi

t1

654

DARNALL TABLE Protein

AND KLOTZ I-Continued

Source

Molecular weight

Subunits Molecular weight

NO.

Z-Keto-3-deoxy-6.phosphogluconate aldolase (EC 4.1.2.12) (86) Malate dehydrogenase (EC 1.1.1.37) (87) 2,CDiaminopentanoic acid C, dehydrogenase (88) Propylamine transferase (89) L-Histidinol phosphate aminotransferase (EC 2.6.1.9) (90) Tryptophanyl tRNA synthetase (EC 6.1.1.2) (91) Sedoheptulose 1,7-diphosphatase (92) Electron-transferring flavoprotein (93) Chorismate mutase-prephenate dehydrogenase (94) Diacetyl reductase (EC 1.1.1.15) (95) Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) (96) Histidyl tRNA synthetase (97) Cyclic AMP-dependent protein kinase (98) Glycerol l-phosphate dehydrogenase (EC 1.1.1.6) (99) Hydroxyindole-O-methyl transferase (EC 2.1.1.4) (100) Uridine diphosphogalactose 4.epimerase (EC5.1.3.2) (101) Luciferase (102) Phosphotransacetylase (EC 2.3.1.8) (103) Alkaline phosphatase (EC 3.1.3.1) (104) Creatine kinase (EC 2.7.3.2) (105) Alcohol dehydrogenase (EC 1.1.1.1) (106) Aldolase (EC 4.1.2.13) (107) Lombricine kinase (EC 2.7.3.5) (108) Taurocyamine kinase (EC 2.7.3.4) (109) Prephenoloxidase (110) Troponin (111)

L-Erythro-3,5-diaminohexanoate dehydrogenase (112) Histidinol dehydrogenase (EC 1.1.1.23) (113) Gaiactose l-phosphate uridyltransferase (EC 2.7.7.12) (113a) Anthranilate synthase (114) Enolase (EC 4.2.1.11) (115) ATP-Creatine transphosphorylase (116) Glutathione peroxidase (117) Purine nucleoside phosphorylase (EC 2.4.1.2) (118) Histidyl tRNA synthetase (119) Succinate dehydrogenase (EC 1.3.99.1) (120) Glutamate decarboxylase (EC 4.1.1.15)

(121)

Haptoglobin l- l(122) Acid phosphatase (EC 3.1.3.2) (123) Alkaline phosphatase (EC 3.1.3.1) (124) Anthranilate synthetase (125)

Pseudomonas

72,000

3

24,000

72,000 72,000

2 2

37,000 40,000

E. coli Salnonella

73,000 74,000

2 2

37,000 37,000

E. coli

74,000

2

37,000

Candida Peptostreptococcus Aerobacter

75,000 75,000 76,000

2 1 1 2

35.000 41,000 33,000 40,000

76,000 78,000

3 3

26.000 25,000

perm

78,000 78,000

Yeast, rabbit

lUS&

78.000

2 1 1 2

40,000 35,000 40,cco 40,000

Bovine

‘ineal gland

78,000

2

39.000

Yeast

79,000

2

39.000

Photobacterium

79,000

Viellonella E. coli Chicken Horse Yeast Cancer and Homarus Lum bricus Silkworm Rabbit

80,000 80,ooO 80,000 80,000 80,000 80,000

1 1 2 2 2 2 2 2

42,000 37,000 (40,000) 40,000 40,cco 41,000 40,000 40,000

80,000

2 2 1 1 1 2

40,000 40,000 37,000 24,000 20,000 39.300

80,000

2

40,000

80,OQO

2

40,000

81,000

1 1 2 2 4 3

63.000 18,000 42,000 41,300 21,000 28,000

2 1 1 2

42,500 60,000 25.000 44,000

2 2 2 1 1

40,000 42.000 43,000 70,000 14.000

Bovine Clostridium

Beef Chinese hamster, human Salmonella Bovine

Ieart

L ,iver EIrain, erythrocyte

luscle ,iver

temolymph luscle

Clostridium

) E. coli Pseudomonas Rabbit Rabbit Bovine Calf

luscle luscle llood pleen

E. coli Rhodospirillum Mouse Human Neuorspora E. coli Acinobacter

80,000 80,000 80,000

82,000 82,600 83,800 84,600 85,000 85,000

Lrain

85,000 85.000 85,000 86,000 86,ooO

SUBUNIT

CONSTITUTION TABLE

Protein

655

OF PROTEINS

I-Continued

SOWW

Molecular weight

Organ

Subunits NO.

Histidinol

dehydroyenase

(EC 1.1.1.23)

E. coli

High-density

lipoprotein

2

PalXreas

88,000 88,000

SerLlIll

88.000

Chicken Micrococcus E. coli Human

Muscle

88,000 88,000 90,000 90,000

2 1 2 2 2 2 2 2 (3-4

44,000 40,000 23,000 27,000 17.000 43,000 46,000 46.000 30,000

Beef Penicillium Bacillus

Liver

E. coli

90,000 90,000 90,000 47,000 90,000

4 2 2 2 2

22,000 45,000 47,000 24,000 45.000

Pseudomonas Pseudomonas

92,000 92,000

2 2

46.000 52.000

92,000 92,500 92,400 93,000 94.000

2 2 2 2 2

52.000 45.000 46.000 47.000 47.000

94,000 94,000 95,000 95.000 96,000 48,000 96,000

2 2 2 4 2 2 (?I (?) 2 2 2

47,000 47,000 45,000 22,000 48,000 24.000 17.250 15,200 47.000 50,000 50,000

Pyruvate dehydrop:enase (EC 1.2.4.1) (136) Carhoxypeptidase G, (136a) Salicylate hydrox>lase (EC 1.14.1.a) (137) Luciferase (138) Isocitrate dehydro;:enase (138a) Adenylate kinase (EC 2.7.4.3) (139) L-&Hydroxynicotine oxidase (139a) 6.Phosphogluconate dehydrogenase (EC 1.1.1.44) (140) Dipeptidase M (141) Prolyl tRNAsynthetase (141a) Tyrosyl tRNA Synthetase (142) Ceramide trihexosidase (143) a-Amylase (1441 Allophycocyanin

Yeast Bovine

(129)

M-line protein (130) Putrecine oxidase (131) Enolase (EC 4.2.1.11) (131a) Galactose l-phosphate uridyltransferase (132) o-Erythulose reductase (1331 2.Deoxycitrate synthase (134) Purine nucleoside phosphorylase (135)

I

87.000

(126) Enolase (EC 4.2.1.11) (127) Procarhoxypeptidsse A (128)

t

Molecular weight

Liver

Firefly Bacilius Breuibacterium Arthrobacter Sheep

Lanterns

Liver

E. co/i E. coli Baciilus HUman Bacillus

Plasma

40,coo

( 145)

Synechococcus

Glyoxylic acid reductase (145~1) L-Ribulokinase (EC 2.7.1.16) (146) N-Formimino-L-gliltamate iminohydrolase (147) D-Amino acid oxidase (EC 1.4.3.3) (148) Diacetyl (acetoin) reductase (EC 1.1.1.5) (149) Cysteamineoxygenase (EC 1.13.1.22) (150) Galactokinase (EC 2.7.1.6) (151) Citrate synthase (ESC 4.1.3.7) (152) Aspartate aminotransferase (EC 2.6.1.1) (15.3) Seryl tRiXAsynthetase (EC 6.1.1.11) (154) a-o-A-Acetylhexose amidase (1.55) 6-Phosphogluconafe dehydrogenase (EC 1.1.1.44) (156) Glutamyl tRNASynthetase (157)

Spinach E. coli Pseudomonas

Leaf

Pig Aerobacter

Kidney

2 4

50,000 25,000

Horse

Kidney

2

50,000

Yeast Pig Chicken

Heart Heart

4 2 2

23,000 50,( co 50,000

2

50,000

6 2

17,000 51,000

1 1 4 2 2

56,000 46,000 25,000 52,000 50,000

2 6

51,000 17,000

o-Galactose dehydrogenase (158) Glutathionereductase (159) Acid phosphomonoesterase I (EC 3.1.3.2) (160) Hexokinase (EC 2.7.1.1) (161) Nucleoside diphosphokinase (EC 2.7.4.6) (16”) Delta hemolysin (1153)

97,500 98,000 100,000

E. coli Placenta Liver E. coli Pseudomonas Sea urchin Human

E% Prostate

Yeast Yeast

21,000 5,000

Staphylococcus

L

?

L

656

DARNALL TABLE

AND KLOTZ I-Continued

Protein

Organ

Molecular weight

l-

Subunits Molecular weight

NO.

Uridine phosphorylase (164) &Phosphogluconate dehydrogenase (EC 1.1.1.44) (165) Aspartokinase III (EC 2.7.2.4) (166) Rihosephosphate isomerase (EC 5.3.1.6)

:

Rat Human

Liver Erythrocyte

103,000 104,000

4 2

26,000 52,000

E. coli Candida

105,OQO 105,000

2 4

50,000 26,000

Rhodopseudomonas E. coli

105,000

2

50,000

lc6,ooO

2

53,000

(167) Isocitrate

dehydrogenase

(TPN)

(168)

Lipoamide dehydrogenase (EC 1.6.4.3) (169) 20- @-Hydroxysteroid dehydrogenase (EC 1.1.1.53) (170) Glycyl-L-leucine hydrolase (171) Sulfatase A (EC 3.1.6.1) (171a) Ornithine transcarbamylase (EC 2.1.3.3) (172) Concanavalin A (173)

Streptomyces

106,ooa

Monkey OX Streptococcus, Bovine Jack bean

Small intestine Liver Liver

4

107,OOil 107,000 108,000

2 2 3

54,000 50,000 36,000

108,000

2 2 2 8 2

Rat Pig

Erythrocyte Liver Brain

108,OQO 108,oOi? 110,000 110,000

Bovine

Pancreas

110,000

2

54,000 27,000 54,000 13,500 54,000 56,000 53,000 58,000

Yeast

110,000

4

27,000

Pseudomonas Micrococcus

110,000 110,ooo

54,000 29,000 7,000 55,000 56,000

E. co/i

110,000 112,OuO 113,000 113,Oinl

2 3 3 2 2 6 (8)

19,500 (15,000)

Rhodospirillum

114,000

2

56,000

Navy bean E. coli

114,000 115,000

4

115,000 115,000 115,000

2 2

54,000

Lipoxygenase (EC 1.99.2.1) (174) Hemerythrin (175) Glutamine transaminase (176) Tubulin (177)

Soybean Goyingia

Tryptophanyl tRNA synthetase (EC6.1.1.2) (178) Phosphoglycerate mutase (EC 2.7.5.3) (179) Urocanase (180) Histidine decarhoxylase (EC 4.1.1.22) (181) Phosphatase (EC 3.1.3.2) (182) Dihydrolipoyl dehydrogenase (183) Canavalin (184) Adenyosylmethionine decarboxylase (184a) D-Ribulose l$diphosphate carboxylase (185) Lectin (186) DNA modification methylase (187) Monoamine oxidase (188) Sultiteoxidase (EC 1.8.3.1) (188a) DNA Modification enzyme (189) Tyrosine aminotransferase (EC 2.6.1.5) (190) Tyrosine tRNAsynthetase (EC 6.1.1.1) (191) Arginase (EC3.5.3.1) (192) Phosphoglucose isomerase (193) a-Ketoglutaric semialdehyde dehydrogenase ( 194) Agglutinin (195) Aspartokinase (EC 2.7.2.4) (196) L-Asparaginase (EC 3.5.1.1) (197) Leucine tRNA synthetase (EC 6.1.1.4) ( 198) Cyclic AMP-dependent protein kinase (199) Protein toxin B (200) Pyrophosphatase

(EC 3.6.1.1) (201) __-

27,000

Sweet potato E. coli

Tuber

1 1

Rat

( Liver

115,OQo

4

30,000 60,000 55,ooo 60.000 55,000 70,000 45,000 32,000

Saccharomyces

~

116,CiM

4

31,500

Pseudomonas

118,000 119,400 120,000

4 4 2

30,000 30,000 60,000

Soybean Bacillus

120,000 120,000

Proteus

120,000

Yeast

120,000

4 2 2 4 2

30,000 43,000 17,000 30,000 60,000

1 1

78,000 35.000 24,000

Pig i ;:T2FiophageP1

Human

Liver I ~~~~~

Liver

Yeast

Sperm

Pasteurella / E.coli

1 1

1 1

120,000 120,000 24,000 120,000

(5-

6: 2 6

12,000 20,000 ~~

SUBUNIT

CONSTITUTION TABLE

I-Continued

SOWX

Protein

657

OF PROTEINS

Organ

Molecular weight

Seryl tRNAsynthetase (EC6.1.1.11) (201a) Aldolase (EC 4.1213) (202) DDT-Dehydrochlorinase (EC 4.5.1.1) (203) Alkaline phosphatase (EC 3.1.3.1) (204) Tryptophan oxygenase (EC 1.13.1.12) (205) Fatty acylthiokinase I(206) Cyclic AMP-dependent protein kinase (207) Ceruloplasmin (208)

Yeast

Methylmalonyl-CoA mutase (EC 5.4.99.2) (2139) Glutathione reductase (EC 1.6.4.2) (210) Deoxycytidylate deaminase (T-2 bacsteriophage induced) (211) Hybridase (212)

Propionibacterium

124,000

E. coli

124,000 124,001I

Rat

Liver

125,000

Uricase (EC 1.7.3.3) (213) Xanthosine 5’.phosphate aminase (EC 6.3.4.1) (214) Tyrosinase (EC 1.10.3.1) (215) Fructose diphosphatase (EC 3.1.3.11) (216) Glucose B-phosphate dehydrogenase (EC 1.1.1.49) (217) o-Gluconate dehydratase (EC 4.2.1) (218) Ornithine aminotransferase (EC 2.6.1.13) (219) Methylmalonate semialdehyde dehydrogenase (219a) L-Asparaginase (EC 3.5.1.1) (220) Aspartokinase (EC 2.7.2.4) (221) Phosphoglucose isomerase (EC 5.3.1.91 (222) L-Amino acid oxidase (EC 1.4.3.2) (223) Myrokinase (EC 3.2.3.1) (224) L-Asparaginase (EC 3.5.1.1) (225) Aminotripeptidase (EC 3.4.1.3) (226) Aspartate carbamoyltransferase (EC 2.1.3.2) (22’1) Lysine tRNA synthetase (EC 6.1.1.6) (228) Nucleoside diphosphokinase (229) L-Asparaginase (EC 3.5.1.1) (230)

Pig E. coli

Liver

Transketolase (EC 2.2.1.1) (231) Protein phosphokinase (EC 2.7.1.37) (232) Succinyl-CoA synthetase (EC 6.2.1.5) (233) C-Reactive protein (234) Lactate dehydrogenase (EC 1.1.1.27) (235) L-Rhamnulose l-phosphate aldolase (EC 1.4.2.b) (236) Ascorbateoxidase (EC 1.10.3.3) (237) Cyclic GMP protein kinase (238) Cyclic AMP-depen,dent (239)

protein kinase I

Spinach Housefly

No.

Molecular weight

2

60,000

Leaf

30,000 30,000

Bacillus

55,000

Pseudomonas

31,000

E. coli Rabbit

Muscle

122,000 123,000

Human

Serum

124,000

Mushroom Swine

Kidney

Rat

Mammary

4 1 1 2 2 1 1 2 6

30,000 82,000 49,000 53.000 16.000 66,000 61,000 56,000 20,200

125,ooO 126,000

1 1 4 2

85,000 43,000 32,000 63,000

128,000 130,ooo

4 4

32,000 34,000

2

63,000

2

64,000

glar

Clostridium Rat

Liver

Pseudomonas E. coli Fseudomonas Human. rabbit Rattlesnake Rape seed Erwinia Swine Yeast

Venom

Kidney

Kidney

Brain

E. coli Blood Heart

E. coli

Beef

132,000

59,000 4 3 2

33,000 43,ooo 61.000 70,000 65.000 32,500 71.100 21.000

135,OQo 135,000 135,Oixl 137,200 138,000 138,GQO

2

72.000

138,Olnl 138,Oinl

6 4

21.000 35,000

140,00( 140,000

2 1 1 2 2 6 4

70,000 80,000 60,000 38,500 29,500 23,000 35,000

140,000

Rabbit Porcine

Zucchini Lobster

33,000

Muscle

Yeast Pig Achrombacteraceae Yeast Bovine

132,000

140,000 140,000 140,000

35,000

Muscle

140,000 140,000

Brain

140,ooo

65,000 100,000 40,000 100,000 40,000

squash

I DARNALL

658

TABLE Source

Protein

AND KLOTZ I-Continued

7

Molecular weight

T

Subunits No.

M&CUl~~

weight Phytohemagglutinin

140,000

35,000 36,000 36,000

140,000 140,000

2 4

70,000 35,000

140,000 140.000 68,000 140,000 141,000

4 2 2 2 4

35,000 68,000 37,000 69,000 35,000

141,000 143,000 144,000 72,000 145,000

2 2 4 2 2 4

60.000 21,000 37,000 72,000 37.000 39,000

B&k&S

145,000 146,000 148,000

4 2 4

35,000 75,000 37.000

E. co/i

148,000 148.000 150,000 150,000

2 2 4 2 4

45.000 28,700 37.000 75.000 38,000

150,000

4

37.000

Phaseolus

(240)

Fructose l,&diphosphatase (EC 3.1.3.11) (241) Esterase (242) 3,4-Dihydroxyphenylacetate-2,3dioxygenase (EC 1.13.1) (243) Aldolase (EC 4.1.2.13) (244) L-Erythro-3,5-diaminohexanoate dehydrogenase (245) Alkaline phosphatase (EC 3.1.3.1) (246) Alcohol dehydrogenase (EC 1.1.1.1) (246~1) Anthranilate synthetase (247) Lectin (248) Glyceraldehyde 3.phosphate dehydro. genase (EC 1.2.1.12) (249) Tart&c acid dehvdrase (EC 4.2.1.~) (250) _ Phosphofructokinase (EC 2.7.1.11) (251) Platelet factor XIII (252) Malate dehydrogenase (EC 1.1.1.37)

-t

2 2 4

Rabbit Rat Pseudomonas

140,000 Liver, kidney, muscle Liver

E. coli Clostridium Calf Yeast

Intestine

Serratia Navy bean Rabbit, lobster Pig Pseudomonas Clostridium Human

MUSCk

Platelet

(253) Tryptpphan

synthetase

(EC 4.2.1.20)

(254) Aldolase C (255) 5’.Nucleotidase (EC 3.1.3.5) (256) Pyridoxamine pyruvate transaminase (EC 2.6.1.a) (257) Alcohol dehydrogenase (EC 1.1.1.1)

(258)

Rabbit Mouse Pseudomonas

Brain Liver

Yeast Bovine

Heart, kidney

154.000

a-Acetylgalactosaminidase (260) Aspartic /3 -semialdehyde dehydrogenase (EC 1.2.1.11) (261) o-Xylose isomerase (EC 5.3.1.5) (262) Crotonase (EC 4.2.1.17) (263) Aldolase (264) Lac repressor (265) Cystathionine y-synthetase (EC 4.2.1.21) (266) 3-Deoxy-o-Arahinoheptulosonate 7.phosphate synthetase-chorismate mutase (266a) Cystathionase (EC 4.2.1.15) (267) Trimethylamine dehydrogenase (268) Threonine deaminase (EC 4.2.1.16) (269) Pyruvate kinase (EC 2.7.1.40) (270) Phosphoglycerate dehydrogenase (2711 Succinic semialdehyde dehydrogenase (EC 1.2.1.b) (2’72) Crotonase (EC 4.2.1.17) (273) Quinolate phosphoribosyltransferase (273a) Carboxyl &erase (EC 3.1.1.1) (274) Tryptophan oxygenase (EC 1.13.1.12) (275) Palmitoyl-CoA synthetase (EC 6.2.1.3) (276) Molybdoferredoxin (277)

Beef Yeast

Liver

155,000 156,000

Aspartokinase III-homoserine genase II (278)

Pyruvate

dehydrogenase

(259)

dehydro-

Streptomyces Clostridium Rabbit E. co/i Salmonella

Muscle

157.000 158.000 160,000 160,000 160,000

41.000 36,000 42,000 41,000 4 4 4 4 4

40,noo 43,000 40,000 40,000 40,000

Bacillus

160,000

4

38.500

Rat Bacterium 4B I3 Clostridium Yeast E. coli Pseudomonas

Liver

160,000 160,000 160,000 161,000 163,000 164.000

8 2 4 8 4 3

20.000 80,000 40,000 20,000 41,000 54.500

Beef Pseudomonas

Liver

164,000 165,000

6 3

28,000 54,000

Beef Rat

Liver Liver

167,000 167.000

Rat

168,000

Clostrrdium

168,000

E. coli

169,000

85,000 43.000 44,000 27,000 2 1 4

59,000 50,700 43,000

SUBUNIT

CONSTITUTION TABLE

659

OF PROTEINS

I-Continued Subunits

Molecular weight

Organ

F’rotein

MOlWUl~I

weight

Acetoacetyl CoA thiolase (EC 2.3.1.9) (279) Thiolase (EC 2.3.1.!3) (279a) Methionine tRNA synthetase (EC6.1.1.10) (280) @-Lysine mutase (231)

Prvian

,iver

169,000

F‘ig 1i. coli

k3rt

169,ooO 170,Ooa

180,000 180,000 180,000

2 2 1 1 4 2 2 3 2 (3-4 )

52,000 32,000 130,000 42.000 45.000 49,000 38,000 60,COO 95,000 50,000

180,000 183,000

6 6

29,oQo 81,500

184,000 186,000 188,000 190,000

4 3 4 5 5 5 4 4 2 4 4

46,000 62,000 47,000 9,000 29,700 37,000 48,500 48,500 100,OQo 24,500 49,200

2 2 2 4 2 2 2 1 1 4 4 4 3 2 6 4 2 2 4

100,000 50,000 95,000 50,000 100,000 48,000 100,000 70,000 30,000 50,ooo 50,000 50,000 70,000 100,000 33,000 50,000 100,000 50,000 52,500

4 2 2 1 1 1 1 4 2 2 4 2

51,000 101,400 51.300 70,COO 65,000 45,000 35,000 48,200 104.000 57,000 50.500 108,OQO

(Xostridium

170,000

Carbamylphosphate synthetase (EC 2.7.2.5) (282) Aspartase (EC 4.3.1 .l) (283) Cyclic AMP-dependent protein kinase (284) Carboxylesterase (EC 3.1.1.1) (285) D-a-Omithine 5,4-aminomutase (286) Thetin homocysteine methylpherase (EC 2.1.1.10) (287) Carbonic anhydrase (287a) L-Leucine:Z-oxoglutarate aminotransferase (EC 2.6.1.6) (288) L-Threonine dehydlatase (289) Aminoacyl transferase I (290) a-Dialkyl amino acid transaminase (291) Histidine decarborylase (EC 4.1.1.22) (292) Prohistidine decarboxylase (293) Fumarase (EC 4.2.1..2) (294) Threonine deaminase (EC 4.2.1.16) (295) Dipeptidyl transferase (EC 3.4.4.9) (296)

13. coli

170,000

Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (29’7) Glutamine phosphoribosylpyrophosphate amidotransferasc (EC 2.4.2.14) (298) Polynucleotide phosphorylase (299) u-Isopropylmalate ‘synthase (300 Aspartylkinase (lysine sensitive) (EC 2.7.2.4) (301) Succinic dehydrogenase (EC 1.3.99.1) (302)

! hlmonella

Neuraminidase (EC 3.2.1.18) (303) Tyrosinase (EC 1.10.3.1) (304) MO-Fe Protein (305) Peroxidase (305a) Cytochrome oxidase (305b) Aliphatic amidase IEC 3.5.1.4) (306) ,%Amylase (EC 3.2.1.2) (307) Argininosuccinase tEC 4.3.2.11 (308)

1‘hflUC?~Za 1‘rag !joybean I ‘ig 13ovine 1h-eudomonas sweet potato steer

NAD-Linked malic enzyme (EC 1.1.1.38) (309) Threonine deaminase (EC 4.2.1.16) (310) Glucose 6.phosphaw dehydrogenase (EC 1.1.1.49) (311) Q@ Replicase (312)

E. coli

Isocitrate lvase (313) Glucose 6-;hospha,.e dehydrogenase (EC 1.1.1.49) (314) Tryptophanase (EC 4.2.1~) (315) Pyruvate decarboxvlase (EC 4.1.1.1) ~(316)

Pseudomonas Neurospora

1?. coli I sovine

Heart

I ‘ig (Xostridium I

Liver

I ‘Llrdey ! ialmonella

Leaf

(Tlostridium I Eabbit 1kwdomonos 1htobacillus 1hctobacillus 5swine >Mmonella I 3eef

I

Liver

Reticulocytes

Heart Spleen

Liver

15. coli !%llmonella 15. coli I

E. coli Human E. coli

Bacillus Yeast

Heart

Epidermis Nodule rhyroid Heart Tuber Liver

Erythrocyte

170,000 174,000

190,000 194,000 195.000 197,ooo 100.000 198,OQO 200,000 100,000 200,Oilo 200,OQO 200,000 100,000 2cO,OoO 100,CQO 200,000 200,000 200,000 200,000 200,Olxl 200,000 201,000 202,cCKl 100,000 200,000 204,ooO 204,800 101,4Oil 205,OQc

206,OOc 206,cOc 104,oOC 208,OOC 209,ooc

660

DARNALL TABLE

I-Continued

Source I

I

Protein

AND KLOTZ

Organ

Subunits

Molecular weight

Invertase (EC 3.2.1.26) (317) Phenylalanine hydroxylase (317a) Cytidine triphosphate synthetase (EC 6.3.4.2) (318) High density protein (319) Phosphoribosyl ATP pyrophosphate phosphoribosyl transferase (320) Serine transhydroxymethylase (EC 2.1.2.1) (321) Acetyl-CoA carboxylase (EC 6.4.1.2) (322) Pyruvate kinase (EC ‘2.7.1.40) (323) a-Gluaan phosphorylase (EC 2.4.1.1) (324) 2.Oxoglutarate dehydrogenase (325) Tryptophanase (EC 4.2.1~~) (326) a-L-Fucosidase (327) Glycerol kinase (EC 2.7.1.30) (328) Adenosine deaminase (EC 3.5.4.4) (328a) Acetol acetate-forming enzyme (329) Tryptophanase (EC 4.2.1.e) (330) Paramyosin (331) Chorismate mutase prephenate dehydratase (332) Sucrase-isomaltase complex (EC 3.2.1) (333) ATPase (Na and K dependent) (334)

Neurospora Rat E. coli

Liver

Porcine Salmonella

Plasma

Rabbit

Liver

Rat

Liver

Bovine Potato Pig A~~OIiXOllfX Rat

215.000

4

Liver

215.000 215.000

1 1 4 2

118,000 125,000 54,000 108,000

Heart

216,000 216,000 216.000

2 4 2

105,000 54,000 ~ 47.000

21i.000 217.000

2

105,000

4 2 2 2 2

58,000 110.000 55.000 110.000 109.000 1lO.( (IO 120,000 (13.5.000) (:‘5.000) 55.000

Epididymes

Aerobacter Bactllus, E. coli

220,000 220.000 110,000 220.000 220.000

I

i ! i I

I

37,onn

a-Acetohydroxyacid isomeroreductase (335) L.(+ ).Hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.351 (336) Pyruvate kinase (EC 2.7.1.40) (337) Arylamidase (338) Ornithine transcarbamylase (EC 2.1.3.3) (339) Ribonucleoside diphosphate reductase (340) Glycyl tRNA synthetase (EC 6.1.1x) (341) 4.Aminobutanal dehydrogenase (EC 1.2.l.e) (342) Cysteine desulfhydrase (343) Pyruvate kinase (EC 2.7.1.40) (344) Glyoxylate carboligase (EC 4.1.1.b) (345)

Salmonella

220,000

1 1 1 2 4

Clostrldium

220.000 ~

8

26.000

220,000 223.500 223,000 74.000 225.000

4 6 3 2 2 2 2 2 3

5c5,.000 38.100 74,000 38,000 85,000 35,Ml 33,000 80,000 75,000

Collagen (345a) C-phyocyanin (346)

Chicken Synechococcus

Leg tendon

Catalase (EC 1.11.1.6) (347) Cytochrome b, (348) Pyruvate kinase (EC 2.7.1.40) (349) Formyltetrahydrofolate synthetase (EC 6.3.4.3) (350) &Aminolevulinate dehydratase (EC 4.2.1.24) (3513 Anthranilate synthetase complex (352) Protein toxin A (353)

Bovine Yeast Rabbit Clostridium

Liver

RhodopseudomORaS Neurospora Pasteurella

Protocollagen

Chick

proline hydroxylase

(354)

Rabbit

Small intestine

220.000

Canine

Renal medulla

(220.000)

Frog Human

StreptococFus, Bovine Bacteriophage

Ml&e Liver Liver

T4

227,000 Pseudomonas

228,000

Salmonella Bovine Pseudomon;s

229,000 230,000 230,000 115,000 : 231.000 232,000

Embryo

232,000 235,000 237,000 240.000

6 4 2 2 12 6 6 4 4 4 4

37,000 57.000 11.5.000

2 :1 6 10-1: 2 2 2

120,000 40,000 40,000 24.000 12.000 60,000 65.000

18,500 19,000 17,iOO 57,500 57,oco 57,200 60.000

--

SUBUNIT

CONSTITUTION TABLE

I-Continued

Source

Protein

Glucocerehrosidase (355) Pyruvate kinase (EC 2.7.1.40) (356) 2,5-Dihydroxypyridine oxygenase (357) Aldehyde dehydrogenase (EC 1.2.1.31 (3,581 Rihonucleotide diphosphate reductase (359)

HUman E. coli Pseudomonas HOlWZ

Melilotate hydroxylase (360) Glycogen synthase (EC 2.1.1.11) (361) DNA restriction endonuclease (462)

Bacterial Rabbit E. coli

Cystathioninesynthetase (EC 4.2.1.21) (363 1 Uridine diphosphat e galactose 4. epimerase (EC.5.1.8.2) (364) Malic enzyme (EC 1.1.1.39) (3651 Phytochrome (366) Leucine aminopeptidase (EC Z1.4.1.I 1 (3671 Malic enzyme (EC 1.1.1.401 (3681 Butyrylcholinesterase (EC 3.1.1.8) (369) L-Phenylalanine tRNA synthetase (EC 6.1.1.h) (370) Glutamine phosphoribosylpyrophosphate amidotransferase (EC 2.4.2.14) ( 37 1 I Glycollateoxidase (EC l.l.Xll (1172) Phenylalanine tRNA synthetase (EC6.l.l.bl (373) AMP deaminase IEXZ 3.5.4.6) (374) Mandelate racemase (EC 5.1.2.21 (37.5) Acetvlcholinestera~;e (EC X.1.1.7) (376) Pro& kinase (377)

661

OF PROTEINS

Orgall

Placenta

Liver

E. culi

Molecular weight

T NO.

Molecular weight

240,ooo 240,OiXl 242,000 245,000

4 4 6 4

60,( 00 60,000 39,500 57,000

245,000

1 1 1 4 (3) 1 1 1

78,000 80,000 80,000 64,000 90,ooo 135.000 60,000 55,000 51,000 74,000 125,000 60,000 64,000 42,000 63,500

250.000 Muscle

Subunits

(250,ooO) 250,000

Liver

250.000

(2) (2)

Ascaris Rye, oat Swine

Muscle Shoots Kidney

250,000 125,000 250,W.M 252,000 255,000

2 2 4 6 4

Pigeon Horse

Liver SWUITI

260,000 260,000

4 4-6

267,000

2 2 2

65.000 42,200 75,000 94,000 39,000 133.000

140,000 75,000 63,000 69,000 69,500 70,000 42,000 55,000 75,000 62.000 62,000 140,000 35.000 64,600

Yeast

E. co/i Placenta

2io.ooa

Spinach Yeast

Leaves

270,000 276,000

Rahhit, chicken Pseudomonas Electrophorus Bovine

MWZle

278,000 278,000 280,000 280,000

6.Glucuronidase (EC B.2.1.31) (3781 Anthranilate synthetase complex (1X9)

Rat Salmonella

Liver

280,900 280.000

B-Aminolevulinic acid dehydratase (EC -1.2.1.24) (380) Glucose-G-phosphate dehydrogenase (EC 1.1.1.49) (381) Lysine 2.~1.amino mutase (382) Glutamate dehydrogenase (EC 1.4.1 .:i) (383) Dopamine-8.hydroxylase (EC 1.14.2.1) (384) Nitrogenase (385)

Bovine

Liver

282,000

Bovine

Adrenal gland

284,000

2 2 2 4 4 4 (?I (?I 4 2 2 2 4 4

285,000 288,400

6 6

48,000 48.800

290,000

4

75,000

295,000

66,700 296,000

1 1 2 2 2 4

229,000 66.700 51,300 59,600 34,000 75.000

300,000

6

52,000

Tis.We Heart

Clostridium NtWYJSpO~f2 Bovine

4drenal gland

Klebsiello

229,000

Areinine

decarhonlase

(EC 4.1 1.191

Uridine diphosphate glucose dehydrogenase (EC 1.1.1.22) (387) Glycogensynthetase (EC 2 4.1.11) (388) Ed&in (389) Excelsin (3%)) lsocitrate dehydrogenase (EC 1.1.1.41) (391) Monoamine oxidaw (391a)

E. coli Bovine

Liver

Yeast Hemp Brazil nut yeast Rat

Seed

i

Liver

300,oOiI 300,000 300,ow 300,ow 300.000

77,000 50,000 50,000 39,wo 4

75,000

662

DARNALLANDKLOTZ TABLE

I-Continued

Protein

Cysteine synthetase Aspartyl (393)

Glutamate

Organ

(EC 4.2.1.2‘2) (392)

transcarbamylase

decarboxylase

(EC 2.1.3.2)

(EC 4.1.1.15)

(394) Cholinesterase (EC 3.1.1.8) (395) Carbamoylphosphate synthase (EC 2.7.2.5) (396) Glutamatedehydrogenase (EC 1.4.1.3) (397) Plasma factor VII (398) L-Phenylalanine ammionia lyase (EC 4.1.3.5) (399) Chloroplast-coupling factor (400) Leucine aminopeptidase (EC 3.4.1.1) (401) Nitrogenase (402)

Molecular weight

T

Subunits No.

Molecular weight 160,OCil 68,000 100,000 34,000 33,000 17,000 50,000

Salmonella

309,oOQ

E. coli

310,000

E. coli

100,000 34,cQo 310,000

1 2 2 3 3 2 6

Liver

315,000 316,000

4 2

77,300 160,000

Liver

320,000

6

57,000

Plasma

320,000 320,000

2 2 4

75,000 88,000 83,000

325.000 327,CiXl

6 6

62,000 54,000

330,OilO

55,000 330,000

1 2 2 2 2 6

220,000 55,000 50,700 59,500 27,500 51,500

335,000 340,000 62,000 340,000

8 6 2 4

42,000 62,000 29,000 85,000

340,000

2

160,000

Erythrocyte Neural retina

340,000 340,wil 340,cQa 345,000 180,000 350,000 350,000

Liver

352,OOil 355,000 360,000

6 8 6 2 6 12 3 3 1 8 8 12-2‘

Horse Rat

Human Maize, potato, wheat Spinach Bovine

Chloroplasts Lens

Clostridium

220,000

Glutamate dehydrogenase (EC 1.4.1.3) (403) Glutamine synthetase (EC 6.3.1.2) (404) Acetoacetate decarboxylase (EC 4.1.1.4) (405) Aspartokinase I-homoserine dehydrogenase I (406) Phosphoenolpyruvate carboxylase (EC4.1.1.31) (407) Peptidase (408) Glutaminesynthetase (EC 6.3.1.2) (409) Aminopeptidase (410) Arachin (411)

Sheep Chicken Clostridium Arachis

N-Methylglutamate synthetase ATPase (EC 3.6.1.3) (413)

Pseudomonas Micrococcus

Hamster Clostridium

Liver

Maize

Glutaminesynthetase (EC 6.3.1.2) (414) Enolase (EC 4.2.1.11) (415) Ptycocyanin (416)

Rat Thermus Anacystis,

Phosphofructokinase (EC 2.7.1.11) (417) L-Arabinose isomerase (EC 5.3.1.4) (418) Glutamine synthetase (EC 6.3.1.2) (419) Aspartate transcarbamylase (EC 2.1.3.2) (420) ATPase (EC 3.6.1.3) (421)

Rabbit E. coli Neurospora Pseudomonas

Muscle

360,000 360,000 360,000 360,000

4 6 4 2

60,000 42,OOQ 60,000 180,000 30,000 (30,000) 52,500 47,000 41,000 44,000 44,000 15,00030,000 80,000 60,000 90,ooa 180,000

Rat

Liver

360,000

Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (422) o-Ribulose-l$biphosphate carboxylase (EC 4.1.1.39) (423) Glycogen synthetase (EC 2.4.1.11) (424) Ptosphorylase A (EC 2.4.1.1) (425) High density lipoprotein (426) Polysaccharide depolymerase (426a)

E. coli W

361,000

6 1 1 1 4

53,000 28,000 12,500 9,000 88,200

Chlorobium

361,000

6

53,000

370,000 370,ooo 376,000 379,000

4 4 4 4 (3-4‘

92,000 92,500 28,000 63,200 36,400

(412)

Swine Rabbit Bovine Aerobacter

Nostic

Kidney MUSCk

Serum

SUBUNIT

CONSTITUTION TABLE

I-Continued Organ

Source

Protein

663

OF PROTEINS

Molecular weight

Subunits No.

Molecular weight

Rat

Liver

4cQ.cixl

Cholesterol &erase (EC 3.1.1.13) (435) Lipovitellin (436) Glutamine synthetase (437) Phosphoenolpyruvate carhoxylase (EC 4.1.1.31) (438) Sucrose synthetase (EC 2.1.1.14) (439) Phosphoenolpyruvate carboxytransphosphorylase (EC 4.1.1.38) (440) Ribonucleotide reductase (440a) Adenosine triphosphate sulfurylase (EC 2.7.7.4) (441:~ Apoferritin (442) RNA polymerase (443)

Rat

Pancreas Egg yolk Brain

400,000 400,000 400,OGil 402,ooO

12 2 2 4 2 10 2 8 4 6 6 2 1 1 1 1 1 1 6 2 8 4

405,000 408,000 200,000 440,000 440.000

4 2 2 4 8

443,000 460.000

RNA polymerase

Bacillus

24 1 1 1 2 1 1 1 1 2 2 2-3 2 3 8

18,500 200,000 135,000 45,000 24,OQO 17,000 160,000 1.5.5.000 63,000 44.000 212,000 20,000 240,000 83.000 6%~

4 1 1 2 12-I

123,000 240,000 240,000 240,000 40,700

4 4 8 6 2 8 2 2 2 8 8 6 12

130,000 135,000 52,000 24,500 12,000 67,000 290.000 137,000 74,000 58,200 15.100 100,000 48,600

Phosphoenolpyruvate carboxylase (EC4.1.1.31) (429) Aminopeptidase IA (EC 3.4.11.1) (4301

385,000 387,000 195,000 400,000 100,000 400,000

AminopeptidaseIB

400,000

ATPase (EC 3.6.1.3) (427) Pyruvate phosphate dikinase

Aminopeptidase

(428)

(EC 3.4.11.1) (431) IC (EC 3.4.1l.l.)

RNA polymerase

(EC 2.7.7.6) (433)

RNA polymerase

(EKZ 2.7.7.6) (434)

Myosin

400,000

(432)

(444)

(445)

400,000

Chicken Ovine E. coli B Phaseolus E&amoeba ELLglUI4l Penicillium Horse Physarum

Rabbit

SplE!tXl

466,000

Muscle

468,000

Urease (EC 3.5.1.5) (446)

Jack bean

Uridine diphosphate glucose pyrophosphorylase (447) Isocitrate lyase (EC 4.1.3.1) (448) Fatty acid synthetase (449)

Calf

Liver

480,000 240,000 480,ooO

Turbatrix Pigeon

Liver

480,000 480,000

Fatty acid synthetase (450) Rihulose 1,5-diphosphate carboxylase (EC 4.1.1:39) (451) Pyruvate carboxylase (452) @-Galactosidase (E’C 3.2.1.23) (453) Ribulose diphosphate carboxylase (EC 4.1.1.39) (451)

Chicken Hydrogenomonas

Liver

500,000 515,000

Pig E. co/i Spinach

Liver

520,000 540,000 550,000

Leaves

L-Malic enzyme (EC 1.1.1.40) (455) Citrate lyase (EC 4.1.3.6) (456)

E. coli Aerobacter

Ribulose 1,5-diphosphate carboxylase (EC 4.1.1.39) (457) Phosphofructokinaje (EC 2.7.1.11) (458) Glutaminesynthetase (EC 6.3.1.2) (459)

Chlorella

24.500 550,ooo 575,000 290,000 137,cnxl 588,000

Yeast E. coli

590.00.1 592,000

33,000 195,Oilo 94,000 100,000 50,000 36,000 36,000 36,000 36,000 36,000 36,000 39,000 155,000 165,000 190,000 150,003; 35,000 I: 25,000 65,000 200,000 49,060 99.600 94,000 200,000 100,000 100,000 ,56,000

DARNALL

664

TABLE

AND KLOTZ I-Continued

Source

Organ

Glyceraldehyde3-phosphatedehydro.yznase(460) Acetolactate synthase (EC 4.1.3.18) (461)

Spinach

Glutaminesynthetase (EC 6.3.1.2) (462) O~omacroglobulin (463) Globulin (464)

Eacilrus Chicken French bean

Egg white

Pyruvate

Chicken

Liver

Bovine Bovine

Thyroid Heart

carboxylase

(EC 6.4.1.1) (465)

Thyroglobulin (466) Isocitrate dehydrogenase (EC 1.1.1.41) (467) L-Asp&ate ,%decarboxylase (EC4.1.1.12) (468) Sulfite reductase (EC 1.8.1.2) (469) Propionylcarboxylase (EC 6.4.1.3) (470) Lysine decarboxylase (EC 4.1.1.18) (471) RNA polymerase (EC 2.7.7.6) (472) Transcarboxylase (EC 2.1.3.1) (473)

Chloroplasts

Pseudomonas

Heart

Molecular weight

600,000

4

145,000

600,OOil

(8) (81

600,OOJJ 650,000 654,ooO 163,000

12 2 4 1 1 1 4 4 2 2 8 6 2 4 8 4 10 2 1 3 3 2 2 2 2 2 2 2 2

1,000,000 1,200,000 1,330,OOa

(24) (8)

1,390,ooo 1,700.000

Gl 24

60,000 60,000 50,000 325,000 163,000 43,000 47,000 53,000 165,000 45,000 335,000 330,000 41,000 112,000 57,000 53,000 58,000 175,000 80,000 391,000 360,OQO 144,000 120.000 60,000 60,000 12,000 400,000 210,000 1oo,coo 60,000 400,000 100,000 26,000 160,000 82,000 470,000 53,000 440,000 760,000 430,000 380,000 74,000 70,000 41,000 146,000 118,000 108,000 41,000 250,COO 6?.000

:i 12 24 12 4 162 60 180 24 24 12

250,000 9%~ 54,000 42,000 230,ooO 54,cOo 18,500 52.000 13,750 96,000 65,000 56cQo

700,000 780,OiXl 782,ooO 792,000 360,000 120,ooo 144.000

Phosphofructokinase

(EC 2.7.1.11) (474)

L-Asp&ate @decarboxylase (EC4.1.1.12) (475) a-Crystallin (476) Arginine decarboxylase (EC 4.1.1.19) (477) Cytochrome P-450 (478)

Chicken

Liver

E. coli Bovine

RNA polymerase (EC 2.7.7.6) (479) Hemocyanin (480)

E. coli B Loglio, Cancer, ghost shrimp

Lipoate succinyltransferase (481) Monoamine oxidase (482) Phosphorylase kinase (EC 2.7.1.38) (483)

Pig Pig Rabbit

Fatty acid synthetase (484) Dihydrolipoyl transacetylase (EC 2.3.1.12) (485) Cblorocruorin (486) a-Ketoglutarate dehydrogenase (487)

Mycobacterium E. coli

Adrenocortical mitochondria

Heart Liver MU&

Spirographis E. coli

Blood

Hemoglobin(488)

Arenicola

Blood

Erythrocruorin(489) Dihydrolipoyl transacetylase (490) Phage fI1 (491) Pyruvate dehydrogenase complex (492)

Cirrajormia Bovine

Plasma Kidney,

complex

E. coli

800,ooQ 4OQ.Oal 210,000 100,cQo 800,000 400,000 810,ooO 820,000 160,000 850,000 470,000 880.000 300.0009,ooo.ooo

2,750.OOO 2,784,OOO

heart

Subunits No.

660,000 165,000 669,000 670,000 330,000 675,000 112,000 700,000

Alcaligines E. co/i. Salmonella Pig E. coli Arotobacter Propionibacteriw

Molecular weight

2,850,OOO 230,000 3,000,ooo 3,120,OOQ 3,620,OOO 4,600,OOO

,3:, 5 2 2 8 2

4 4

SUBUNIT

CONSTITUTION TABLE

Protein

665

OF PROTEINS

I-Continued

Source

Molecular weight

T

Subunits NO.

Cowpea chlorotic mottle virus (492) Bromgrass mosaic virus (494) Broad bean mottle virus (495) Turnip yellow mosaic virus (496) Poliomyelitis virus (497) Cucumber mosaic virus (498) Alfalfa mosaic virus (497) Acetyl CoA Carboxylase

(500)

Chicken

Liver

4,608,OOO 4,600,OOO 4,800,OOO 5,000,000 5,500,OOo 6,000,OOO 7,400,oOO 51,600 4-10,000,000 470,000

180 180 180 150 130 185 (140) (f , 2 1

1 Rhinovirus 1A (501) Bushy stunt virus (502) Poiyoma virus (503) Potato virus X (504) Tobacco mosaic virus (505)

8,400,OOO

9,000,000 24,000,OOO

35,000,000 40,000,000

60 120 420 650 2130

Molecular weight 19,600 20,000

20,900 21,000 27,000 21,500 51,600 24,500 470,000 117,000

129,000 139,000 96,000 60,000 50,200 52,000 17,500

666

DARNALLANDKLOTZ

TABLE II ALPHABETICALLISTING OF PROTEINS FROM TABLE I ~______--~ Ascorbate oxidase (237) Acetoacetate decarboxylase (405) Acetoacetyl-CoA thiolase (279) L-Asparaginase (197), (220), (225), (230) a-Acetohydroxy acid isomeroreductase Aspartase (283) Aspartate aminotransferase (153) (355) Acetol acetate-forming enzyme (329) L-Aspartate fl-decarboxylase (468), (475) Acetolactate synthase (461) Aspartic p-semialdehyde dehydrogenase /3-n-N-acetyl amidase (155) (261) Acetylcholinesterase (376) Aspartokinase (166), (196), (221), (301) Acetyl-CoA carboxylase (322), (500) Aspartokinase-homoserine dehydrogenase cz-Acetyl galactosaminidase (260) (433, (278) 0-Acetylserine sulfhydrylase A (71) Aspartyl transcarbamylase (227), (393), Acid phosphatase (123), (160), (182) (420) Adenine phosphoribosyltransferase (20) ATPase (334), (413), (421), (427) Adenosine deaminase (328a) Avidin (77) Adenosine triphosphate sulfurylase (441) Azoferredoxin (46) Adenosylmethionine decarboxylase (184a) Biotin carboxyl carrier protein (35) Adenylate kinase (74), (139) Broad bean mottle virus (495) Agglutinin (25), (195) Bromgrass mosaic virus (494) Alcohol dehydrogenase (49a), (106)) (246a) Bushy stunt virus (502) Aldehyde dehydrogenase (358) Aldolase (80), (107), (102), (244), (255), Butyryl cholinesterase (369) C-Reactive protein (234) (264) Aldose reductase (56) Canavalin (184) Alfalfa mosaic virus (499) Carbamoylphosphate synthase (282), (396) Aliphatic amidase (306) Carbonic anhydrase (287a) Alkaline phosphtase (104)) (124)) (204), Carboxylesterase (274), (285) Carboxypeptidase G, (136a) (246) Allophycocyanin (145) Catabolite gene activator protein (36) o-Amidase (49) Catalase (347) D-Amino acid oxidase (148) Ceramide trihexosidase (143) L-Amino acid oxidase (223) Ceruloplasmin (208) Aminoacyl transferase (290) Chlorocruorin (486) Aminobutanal dehydrogenase (342) Chloroplast coupling factor (400) 6-Aminolevulinate dehydratase (351), Cholesterol esterase (435) Cholinesterase (395) (380) Aminopeptidase (410), (430), (431), (432) Chorionic gonadotropin (29) Aminotripeptidase (226) Chorismate mutase-prephenate dehydroAMP deaminase (374) genase (94), (332) cy-Amylase (144) Chymotrypsin inhibitor (30) P-Amylase (307) Citrate lyase (456) Anthranilate synthetase (114), (125), (247), Citrate synthase (152) Collagen (345a) (352), (379) Apoferritin (442) Concanavalin A (173) L-Arabinose isomerase (418) Creatine kinase (105) Arachin (411) Creatine transphosphorylase (116) Arginase (192) Crotonase (263)) (273) Arginine decarboxylase (386), (477) cz-Crystallin (476) Argininosuccinase (308) Cowpea chlorotic mottle virus (493) Arylamidase (338) Cucumber mosaic virus (498)

SUBUNIT

CONSTITUTION

TABLE

667

II-Continued

Cyclic AMP’-dependent protein kinase (98), (199), (2071, (232), (239, (284) Cyclic GMP protein kinase (238) Cystathionase (261) Cystathionine y-synthetase (266), (363) Cysteamine oxygenase (150) Cysteine desulfhydrase (343) Cysteine synthetase (392) Cytidine triphosphate synthetase (318) Cytochrome h, (348) Cytochrome (CC’) (11) Cytochrome oxidase (305b) Cytochrome P-450 (478) Cytoplasmic protein (37) DDT-dehydrochlorinase (203) Delta hemolysin (163) 2-Deoxycitrate synthase (134) Deoxycytidylate deaminase (51), (211) Dethiobiotin synthetase (32a) 3-Deoxy-D-arabinoheptulosonate 7-phosphate syn-thetase-chorismate mutase (266a) Diacetyl reductase (95)) (149) cu-Dialkylamino acid transaminase (291) 2,4-Diaminopentanoic acid C!, dehydrogenase (88) Dihydrolipoyl dehydrogenase (183) Dihydrolipoyl transacetylase (485), (490) Dihydropteridine reductase (33) 3,4-Dihydroxyphenylacetate-2, 3-deoxygenase (243) Dihydroxypyridine oxygenase (357) Dipeptidase M (141) Dipeptidyltransferase (296) DNA modification enzyme (189) DNA modification methylase (187) DNA polymerase (55) DNA restriction endonuclease (362) Dopamine-P-hydroxylase (384) Edestin (389) Electron-transferring flavoprotein (93) Enolase (115)., (127), (131a), (415) Erythrocruorin (489) Erythrocuprein (59) Erythro-3,5diaminohexanoate dehydrogenase (112), (243) Erythulose reductase (133) Esterase (242) Estradiol dehydrogenase (76)

OF PROTEINS

Excelsin

(390)

Factor X (39) Fatty acid synthetase (449), (450), (484) Fatty acylthiokinase (206) Follicle-stimulating hormone (19), (22) N-Formimino-L-glutamate iminohydrolase (147) Formyltetrahydrofolate synthetase (350) Fructose diphosphatase (216), (241) cy-L-Fucosidase (327) Fumarase (294) Galactokinase (42) n-Galactose dehydrogenase (158) Galactose-l-phosphate uridyltransferase (113a), (132) p-Galactosidase (453) Globulin (464) a-Glucan phosphorylase (324) Glucocerebrosidase (355) Glucokinase (70), (151) n-Gluconate dehydratase (218) Glucose-B-phosphate dehydrogenase (217), (311), (314), (381) @-Glucuronidase (378) Glutamate decarboxylase (121), (394) Glutamate dehydrogenase (383), (397), (403) Glutamine phosphoribosylpyrophosphate amidotransferase (298), (371) Glutamine synthetase (404), (409), (414), (4191, (437), (459), (462) Glutamine transaminase (176) Glutamyl tRNA synthetase (157) Glutathione peroxidase (117) Glutathione reductase (159), (210) Glyceraldehyde-3-phosphate dehydrogenase (249), (460) n-Glycerate dehydrogenase (78a) Glycerol kinase (328) Glycerol-l-phosphate dehydrogenase (99) Glycerol-3-phosphate dehydrogenase (75) Glycogen synthetase (361), (388), (424) Glycohydrolase (85) Glycollate oxidase (327) Glycyl-L-leucine hydrolase (171) Glycyl tRNA synthetase (341) Glyoxylate carboligase (345) Glyoxylic acid reductase (145a) Growth hormone (38)

668

DARNALL

TABLE

AND KLOTZ

II-Continued

Haptoglobin (122) Hemagglutinin LcH (39a) Hemerythrin (31a), (175) Hemocyanin (480) Hemoglobin (21), (62), (78), (488) Hexokinase (161) High-density lipoprotein (lag), (319), (426) Histidine decarboxylase (181), (292) Histidinol dehydrogenase (113), (126) Histidinol phosphate aminotransferase (90) Histidyl tRNA Synthetase (97), (119) Hyaluronidase (47) Hybridase (212) Hydrogenase (52) Hydroxyacyl-CoA dehydrogenase (64) Hydroxybutyryl-CoA dehydrogenase (336) B-Hydroxydecanoylthioester dehydrase (2’3 Hydroxyindole-O-methyl transferase (100) L-6-Hydroxynicotine oxidase (139a) Hydroxypyruvate reductase (83) 20-@-hydroxysteroid dehydrogenase (170) Hypoxanthine-guanine phosphoribosyl transferase (96) Insulin (5) Interstitial cell-stimulating hormone (14) Invertase (317) Isocitrate dehydrogenase (50), (138a), (168), (391), (467) Isocitrate lyase (313), (448) cy-Isopropylmalate synthase (300) 2-Keto-3-deoxy-6-phosphogluconate aldolase (86) a-Ketoglutarate dehydrogenase (487) a-Ketoglutaric semialdehyde dehydrogenase (194) Lac repressor (265) Lactate dehydrogenase (79), (235) p-Lactoglobulin (23) Lactose specific factor (18) Lactose specific phosphocarrier protein (24) Lactose synthetase (53) Lectin (186), (248) Leucine aminopeptidase (367), (401) L-Leucine:2-oxoglutarate aminotransferase (288)

Leucine tRNA synthetase (198) Luteinizing hormone (lo), (la), (15) Lipoamide dehydrogenase (169) Lipoate succinyltransferase (481) Lipoxygenase (174) Lipovitellin (436) Lombricine kinase (108) Luciferase (102), (138) Lysine 2,3-aminomutase (382) Lysine decarboxylase (471) /3-Lysine mutase (281) Lysine tRNA synthetase (228) M-line protein (130) Malate dehydrogenase (44)) (68), (69), (87), (253) Malate-lactate transhydrogenase (81) Malic enzyme (30), (365), (367), (455) Mandelate racemase (375) Melilotate hydroxylase (360) Mercaptopyruvate sulfur transferase (7) Methionine tRNA synthetase (280) 5,10-Methylenetetrahydrofolate dehydrogenase (48) Methylmalonate semialdehyde dehydrogenase (219a) Methylmalonyl-CoA mutase (209) N-Methylglutamate synthetase (412) MO-Fe protein (305) Molybdoferredoxin (277) Monoamine oxidase (188)) (391a), (482) Myosin (445) Myrokinase (224) Nerve-growth factor (9) Neuraminidase (303) Nitrogenase (385), (402) 5’Nucleosidase (256) Nucleoside diphosphate kinase (84), (162), (229) Nucleoside phosphotransferase (34) o-a-Ornithine 5,4-aminomutase (286) Ornithine aminotransferase (219) Ornithine transcarbamylase (172), (339) 2-Oxoglutarate dehydrogenase (325) -3-Oxosteroid isomerase (8) ?J:-dmacroglobulin (463) Palmitoyl-CoA synthetase Paramyosin (331) Peptidase (408)

(276)

SUBUNIT

CONSTITUTION

OF PROTEINS

669

TABLE II-Continued ~--~ Peroxidase (305a) Purine nucleoside phosphorylase (118), Phage F II (4.91) (135) Phenylalanine ammonia lyase (399) Putrecine oxidase (131) Phenylalanine hydroxylase (317a) Pyridoxamine pyruvate transaminase Phenylalanine hydroxylase stimulating (257) protein (41) Pyrophosphatase (65), (201) Phenylalanine tRNA synthetase (370), Pyruvate decarboxylase (316) (373) Pyruvate dehydrogenase (136), (492) Phosphofructokinase (251), (417), (458), Pyruvate kinase (270), (323), (337), (344), (474) (3491, (356) Phosphoenolpyruvate carboxylase (297), Pyruvate phosphate dikinase (428) (407), (422), (429), (438), (452), (465) Quinolate phosphoribosyltransferase Phosphoenolpyruvate carboxytransphos(273a) phorylase (440) Rhamnulose l-phosphate aldolase (236) Phosphoglucomutase (58) 6-Phosphogluconate dehydrogenase (140)) Rhinovirus 1A (501) Rhodanese (28) (156), (16511 Ribonuclease (13) Phosphoglucose isomerase (193), (222) Ribonucleotide diphosphate reductase Phosphoglycerate dehydrogenase (271) (340), (359) Phosphoglycerate mutase (67), (179) Ribonucleotide reductase (440a) Phospholipase A, (14a) Phosphoribosyl ATP pyrophosphate phos- Ribosephosphate isomerase (167) Ribulokinase (146) phoribosyl transferase (320) n-Ribose 1,5-diphosphate carboxylase Phosphorylase A (425) (185), (423), (451), (454) Phosphorylase kinase (483) RNA polymerase (26), (433), (434), (443), Phosphotransacetylase (103) (444), (472), (479) Phycocyanin (40), (346), (416) Phytochrome (366) S-100 protein (6) Phytohemagglutinin (240) Salicylate hydroxylase (137) Plasma factor XIII (398) Sedoheptulose 1,7-diphosphatase (92) Platelet factor XIII (252) Serine dehydratase (60) Poliomyelitus virus (497) Serine transhydroxymethylase (321) Polynucleotide phosphorylase (299) Seryl tRNA synthetase (154), (201a) Polyoma viruls (503) Succinic dehydrogenase (120), (302) Po1ysaccharid.e depolymerase (426a) Succinic semialdehyde dehydrogenase Potato virus X (504) (272) Prealbumin (!j7) Succinyl-CoA synthetase (233) Prephenoloxidase (110) Sucrase-isomaltase complex (333) Procarboxypeptidase A (128) Sucrose synthetase (439) Prohistidine decarboxylase (293) Sulfite oxidase (188a) Prolyl tRNA synthetase (141a) Sulfite reductase (469) Propionyl carboxylase (470) Sulfatase A (171a) Propylamine transferase (89) Superoxide dismutase (16), (17), (31) Proteinase inhibitor I (32) Tartaric acid dehydrase (250) Protein kinase (377) I’aurocyamine kinase (109) Protein phosphokinase (232) I’hetin homocysteine methylpherase (287) Protein toxin A (353) I’hiogalactoside transacetylase (66) Protein toxin B (200) I’hiolase (279a) Protocollagen proline hydroxylase (354) I’hreonine deaminase (269), (295), (310)

670

DARNALL

AND KLOTZ

TABLE II-Corztinued ___________ ~~~~~~ ~~~~ Tubulin (177) Threonine dehydratase (289) Turnip yellow mosaic virus (496) Thioredoxin reductase (67a) T,-T, complex (63) Thymidylate synthetase (61), (82) Tyrosinase (215), (304) Thyrotropin (17a) Tyrosine aminotransferase (190) Thyroglobulin (466) Tyrosyl tRNA synthetase (142), (191) Tobacco mosaic virus (505) Transaldolase III (73) Urease (446) Transcarboxylase (473) Uricase (213) Transcobalamin (54) Uridine diphosphogalactose 4-epimerase Transglutaminase (45) (lOI), (364) Transketolase (231) Uridine diphosphate glucose dehydrogenTrimethylamine dehydrogenase (268) ase (387) Triosephosphate isomerase (43) Uridine diphosphate glucose pyrophosphoTropomyosin B (72) rylase (447) Troponin (111) Uridine phosphorylase (164) Tryptophanase (315), (326), (330) Urocanase (180) Tryptophan oxygenase (205), (275) Xanthosine 5’-phosphate aminase (214) Tryptophan synthetase (254) Tryptophanyl tRNA synthetase (91), (178) Xylose isomerase (262) REFERENCES 1. DARNALL, D. W., AND KLOTZ. I. M. (1972) Arch. Biochem. Biophys. 149, 1. 2. KLOTZ, I. M., LANGERMAN, N. R., AND DARNALL, D. W. (1970) Ann&. Reu. Biochem. 39, 25. 3. KLOTZ, I. M., AND DARNALL, D. W. (1969) Science 166, 126. 4. KLOTZ, I. M. (1967) Science 155, 697. 4a. KLEINE, V. R. (1972) Forts&r. ArzneimittForsch. 16, 365. 5. CROWFOOT, D. (1938) Proc. Roy. Sot. London Ser. A 164, 580; MOODY, L. S. (1944) Dissertation, University of Wisconsin; WAUGH, D. F. (1954) Aduan. Protein Chem. 9, 325. 6. STEWART, J. A. (1972) Biochem. Biophys. Res. Commun. 46, 1405; DANNIES, P. S., AND LEVINE, L. (1969) Biochem. Biophys. Res. Commun. 37, 587. 7. VACHEK, H., AND WOOD, J. L. (1972) Biochim. Biophys. Acta 258, 133. 8. WEINTRAUB, H., VINCENT, F., BAULIEU, E. E.. AND ALFSEN, A. (1973) Fed. Eur. Biochem. Sot. Lett. 37, 82. 9. ANGELETTI, R. H., AND BRADSHAW, R. A. (1971) Proc. Nat. Acad. Sci. USA 68, 2417. 10. LIU, W.-K., NAHM, H. S., SWEENEY, C. M., HOLCOMB, G. N., AND WARD, D. N. (1972) J. Biol. Chem. 247, 4365; Lru, W.-K., NAHM, H. S., SWEENEY, C. M., LAMKIN, W. M., BAKER, H. N., AND WARD, D. N. (1972) J. Biol. Chem. 247, 4351. 11. CUSANOVICH, M. A., TEDRO, S. M., AND KAMEN, M. D. (1970) Arch. Biochem. Biophys. 141, 557.

12. BISHOP, W. H., AND RYAN, R. J. (1973) Biochemistry 12, 3077. 13. D’ALESSIO, G., PARENTE, A., GUIDA, C., AND LEONE, E. (1972) Fed. Eur. B&hem. Sot. Lett. 27, 285. 14. SAIRAM, M. R.. PAPKOFF, H.. AND LI, C. H. (1972) Arch. Biochem. Biophys. 153,554; SAIRAM, M. R., SAMY, T. S. A., PAPKOFF, H., AND LI, C. H. (1972) Arch. Biochem. Biophys. 153, 572. 14a. WELLS, M. A. (1971) Biochemistry 10, 4074. 15. WARD, D. N., REICHART, L. E., JR., FITAK, B. A., NAHM, H. S., SWEENEY,C. M., AND NEILL, J. D. (1971) Biochemistry 10, 1796. 16. MISRA, H. P., AND FRIDOVICH, I. (1972) J. Biol. Chem. 247, 3410. 17. HARTZ, J. W., AND DEUTSCH, H. F. (1972) J. Biol. Chem. 247, 7043. 17a. HENNEN, G.. MAGHLJIN-ROGISTER, G., AND MAMOIR, G. (1970) Fed. Eur. Biochem. Sot. Lett. 9, 20. 18. SCHRECKER, O., AND HENGSTENBERG, W. (1971) Fed. Eur. Biochem. Sot. Lett. 13, 209. 19. LANDEFELD, T. D., AND MCSHAN, W. H. (1974) J. Biol. Chem. 249, 3527. 20. THOMAS, C. B., ARNOLD, W. J., AND KELLEY, W. N. (1973) J. Biol. Chem. 248, 2529. 21. OHNOKI, S., MITOMI, Y., HATA, R., AND SATAKE, K. (1973) J. Biochem. 73, 717. 22. RYAN, R. J., JIANG, N.-S., AND HANLON, S. (1971) Biochemistry 10, 1321; SAXENA, B. B., AND RATHNAM, P. (1971) J. Biol. Chem. 246, 3549. 23. BULL, H. B. (1946) J. Amer. Chem. Sot. 68, 745; TOWNEND, R., AND TIMASHEFF, S. N. (1957) J. Amer. Chem. Sot. 79, 3613. 24. HAYS, J. B., SIMONI, R. D., AND ROSEMAN, S.

SUBUNIT

CONSTITUTION

(1973) J. Biol. Chem. 248, 941. 25. NAGATA, Y., AND BURGER, M. M. (1974) J. Viol.

Chem. 249, 3116. 26. LOUIS, B. G., AND F~rr, P. S. (1972) Biochem. J. 127, 69. 27. HELMKAMP. G. M., JR., AND BLOCH, K. (1969) J.

Biol. Chem. 244, 6014. 28. VOLINI, M., DETOMA, F., AND WESTLEY, J. (1967)

J. Biol. (“hem. 242, 5220. 29. BELLISARIO, R., CARLSEN, R. B., AND BAHL, 0. P. (1973) J. Biol. Chem. 248, 6796; CARLSEN, R. B., BAHI., 0. P., AND SWAMINATHAN, N. (1973) J. Biol. (Them. 248, 6810. 30. MELVILLE, J. C., AND RYAN, C. A. (1970) Arch. Biochem. Biophys. 138, 700. 31. KEELE, B. B., JR., MCCORD, J. M., AND FRIDOVICH, I. (1970) J. Biol. Chem. 245, 6176. 31a. LIBERATORE, F. A., TRUBY, M. F., AND KLIPPENSTEIN, G. L. (1974) Arch. Biochem. Biophys. 160, 223. 32. KIYOHARA, T., IWASAKI, T.. AND YOSHIKAWA, M. (1973) J. Biochem. 73, 89. 32a. KRELL, K., AND EISENBERG,M. A. (1970) J. Biol. Chem. 2,45, 6558. 33. CRAINE, J. E., HALL, E. S., AND KAUFMAN, S. (1972) J. Biol. Chem. 247, 6082. 34. RODGERS, Ii., AND CHARGAFF, E. (1972) J. Biol. Chem. 247, 5448. 35. FALL, R. R., AND VAGELOS, P. R. (1972) J. Biol. Chem. 247, 8005. 36. RIGGS, A. I)., REINESS, G., AND ZUBAY, G. (1971) Proc. Nat. Acad. Sci. USA 68, 1222. 37. SHANNON, C. F., AND HILL, J. M. (1971) Biochemistry 10, 3021. 38. EDELHOCH, H.,. CONDLIFFE, P. G., LIPPOLDT, R. E., AND BURIGER,H. G. (1966) J. Biol. Chem. 241, 5205. 39. RADCLIFFE, R. D., AND BARTON, P. G. (1972) J.

Biol. Chem. 247, 7735. 39a. HOWARD, I. K., SAGE, H. J., STEIN, M. D., YOUNG, IV. M., LEON, M. A., AND KYKES, D. F. (1971) J. Biol. Chem. 246, 1590. 40. MACCOLL, R., HABIG, W., AND BERNS, D. S. (1973) J Biol. Chem. 248, 7080. 41. HUANG, C. Y., MAX, E. E., AND KAUFMAN, S. (1973) J. Biol. Chem. 248, 4235. 42. BLUME, K.-G., AND BUETLER, E. (1971) J. Biol. Chem. 246, 6507. 43. HARTMAN, F. C. (1971) Biochemistry 10, 146. 44. MUNKRES, K. D. (1965) Biochemistry 4, 2180, 2186. 45. CHUNG, S. I., AND FOLK, J. E. (1972) hoc. Nat. Acad. Sci. USA 69, 303. 46. NAKOS, G., AND MORTENSON, L. (1971) Biochemistry 10, 455. 47. KHORLIN, A. Y., VIKHA, I. V., AND MILISHNIKOV, A. N. (I 973) Fed. Eur. Biochem. Sot. Lett. 31, 107. 48. O’BRIEN, FV. E., BREWER, J. M., AND LJUNGDAHL,

OF PROTEINS

671

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