Parasitol Res DOI 10.1007/s00436-015-4326-1
ORIGINAL PAPER
Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui Kulthida Kopolrat & Paiboon Sithithaworn & Smarn Tesana & Ross H. Andrews & Trevor N. Petney
Received: 24 October 2014 / Accepted: 19 January 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract Fish-borne zoonotic trematodes (FZT) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in Southeast Asia. FZT are reported highly prevalent in juvenile aquaculture fish that belonged to the family Heterophyidae, particularly Haplorchis taichui. Aquaculture fish are reported to have varying levels of natural infection with H. taichui, but data for their susceptibility as well as resistance to infection are not available. The aim of this study was to determine the susceptibility of five aquaculture fish to H. taichui. Experimental infections were performed by exposing fish individually to 0 (control), 50, 100, or 200 H. taichui cercariae for 12 h. Metacercarial burden was measured at 45 days postinfection. Three out of five fish species, silver barb (Barbonymus gonionotus), common carp (Cyprinus carpio), and mrigal (Cirrhinus mrigala), were highly susceptible to H. taichui infection with percentage ranging from 93.33 to 100 %. The Nile and red tilapia were not susceptible to infection. Among the susceptible fish species, K. Kopolrat : P. Sithithaworn : S. Tesana : R. H. Andrews Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand K. Kopolrat : P. Sithithaworn (*) : R. H. Andrews Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand e-mail: [email protected] R. H. Andrews : T. N. Petney Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen 40002, Thailand R. H. Andrews Imperial College London, Faculty of Medicine, St Mary’s Campus, South Wharf Street, London W2 1NY, UK T. N. Petney Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany
silver barb had higher intensity of H. taichui metacercariae than common carp and mrigal (P
ORIGINAL PAPER
Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui Kulthida Kopolrat & Paiboon Sithithaworn & Smarn Tesana & Ross H. Andrews & Trevor N. Petney
Received: 24 October 2014 / Accepted: 19 January 2015 # Springer-Verlag Berlin Heidelberg 2015
Abstract Fish-borne zoonotic trematodes (FZT) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in Southeast Asia. FZT are reported highly prevalent in juvenile aquaculture fish that belonged to the family Heterophyidae, particularly Haplorchis taichui. Aquaculture fish are reported to have varying levels of natural infection with H. taichui, but data for their susceptibility as well as resistance to infection are not available. The aim of this study was to determine the susceptibility of five aquaculture fish to H. taichui. Experimental infections were performed by exposing fish individually to 0 (control), 50, 100, or 200 H. taichui cercariae for 12 h. Metacercarial burden was measured at 45 days postinfection. Three out of five fish species, silver barb (Barbonymus gonionotus), common carp (Cyprinus carpio), and mrigal (Cirrhinus mrigala), were highly susceptible to H. taichui infection with percentage ranging from 93.33 to 100 %. The Nile and red tilapia were not susceptible to infection. Among the susceptible fish species, K. Kopolrat : P. Sithithaworn : S. Tesana : R. H. Andrews Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand K. Kopolrat : P. Sithithaworn (*) : R. H. Andrews Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand e-mail: [email protected] R. H. Andrews : T. N. Petney Cholangiocarcinoma Screening and Care Program (CASCAP), Khon Kaen University, Khon Kaen 40002, Thailand R. H. Andrews Imperial College London, Faculty of Medicine, St Mary’s Campus, South Wharf Street, London W2 1NY, UK T. N. Petney Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany
silver barb had higher intensity of H. taichui metacercariae than common carp and mrigal (P
