Proc. Nat. Acad. Sci. USA Vol. 73, No. 1, pp. 161-164, January 1976
Cell Biology
Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles (de novo protein synthesis/metrizamide-D20 gradients/stable isotopes)
PETER N. DEVREOTES* AND DOUGLAS M. FAMBROUGHt * Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218; and t Carnegie Institution of Washington, Department of Embryology, 115 West University Parkway, Baltimore, Maryland 21210
Communicated by Donald D. Brown, October 17,1975
ABSTRACT Mono - ["25I]iodo- a - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or '3C-substituted amino acids under conditions of active receptor accumulation, the mono[l25Iiodo
Cell Biology
Synthesis of acetylcholine receptors by cultured chick myotubes and denervated mouse extensor digitorum longus muscles (de novo protein synthesis/metrizamide-D20 gradients/stable isotopes)
PETER N. DEVREOTES* AND DOUGLAS M. FAMBROUGHt * Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218; and t Carnegie Institution of Washington, Department of Embryology, 115 West University Parkway, Baltimore, Maryland 21210
Communicated by Donald D. Brown, October 17,1975
ABSTRACT Mono - ["25I]iodo- a - bungarotoxin - receptor complexes extracted from chick myotube cultures as well as from adult denervated extensor digitorum longus muscles of the mouse have been banded at their buoyant density in gradients of metrizamide-deuterium oxide. When cultures or denervated adult muscles are preincubated in media containing 2H- or '3C-substituted amino acids under conditions of active receptor accumulation, the mono[l25Iiodo
