Mitochondrial DNA The Journal of DNA Mapping, Sequencing, and Analysis
ISSN: 1940-1736 (Print) 1940-1744 (Online) Journal homepage: http://www.tandfonline.com/loi/imdn20
The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae) I-Shiung Chen, Zong-Han Wen, Yi-Ta Shao & Chiao-Ru Liao To cite this article: I-Shiung Chen, Zong-Han Wen, Yi-Ta Shao & Chiao-Ru Liao (2015) The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae), Mitochondrial DNA, 26:6, 929-931, DOI: 10.3109/19401736.2013.863295 To link to this article: http://dx.doi.org/10.3109/19401736.2013.863295
Published online: 10 Jan 2014.
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Article views: 16
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Citing articles: 7 View citing articles
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=imdn20 Download by: [York University Libraries]
Date: 06 November 2015, At: 21:33
http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, 2015; 26(6): 929–931 ! 2014 Informa UK Ltd. DOI: 10.3109/19401736.2013.863295
MITOGENOME ANNOUNCEMENT
The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae) I-Shiung Chen1, Zong-Han Wen1, Yi-Ta Shao2, and Chiao-Ru Liao1 Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan, ROC and 2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC
Mitochondrial DNA 2015.26:929-931.
1
Abstract
Keywords
In this study, the complete mitogenome sequence of cyprinid fish, Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope, 1927 (Cypriniformes: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,674 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 1004 bp length is located between tRNAPro and tRNAPhe. The overall base composition of S. hainanensis is 30.0% for A, 26.7% for C, 26.4% for T and 16.8% for G, with a slight AT bias of 56.4%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of cyprinid fishes.
Hainan island, mitogenome, Sarcocheilichthys hainanensis
The cyprinid fishes are highly diverse group in freshwater habitats of SE and E Asia. The freshwater cyrpinid Sarcocheilichthys Bleeker, 1860 is typical freshwater fish in moderate running waters in river basins from Japan, Korea to China southward to Vietnam. The Hainan gudgeon, Sarcocheilichthys hainanensis Nichols & Pope, 1927 may be endemic to the Hainan island, China. The specimen was collected from the Nandujiang river basin, Hainan. A fin tissue was sampled and preserved in 95% ethanol. Total DNA was extracted from the fish tissue using the High Pure DNA Extraction Kit (Roche Applied Sciences, Penzberg, Germany). The mitogenome of S. hainanensis was amplified in its entirety using a long PCR technique (Miya & Nishida, 1999) with 18 pairs of primers designed in accordance with equivalent mitochondrial genome sequences of over 30 species of Cypriniformes (Chen, 2013; Chen & Wu, 2014; Han et al., 2014; He et al., 2012; Huang et al., 2012; Wang et al., 2008, 2011; Yang et al., 2012). The complete mitochondrial genome of S. hainanensis was found to be similar to that of most other vertebrates (Chen, 2013; Chen & Wu, 2013; Chiang et al., 2013). It was amplified and sequenced to be 16,674 bp in size (GenBank accession number KF765805), including 13 typical vertebrate protein-coding genes,
Correspondence: I-S. Chen, Institute of Marine Biology, National Taiwan Ocean University, Keelung 202, Taiwan, ROC. Tel: 886 24622192 ext. 5305. Fax: 886 224633152. E-mail: [email protected]
History Received 29 October 2013 Accepted 3 November 2013 Published online 10 January 2014
22 transfer RNA genes, 2 ribosomal RNA genes and a control region (Table 1). All genes were encoded on H-strand with exception of one protein-coding gene (ND6) and eight tRNA genes (tRNAGln, tRNAAla, tRNAAsn, tRNACys, tRNATyr, tRNASer(UCN), tRNAGlu and tRNAPro). The overall base composition of S. hainanensis is 30.0% for A, 26.7% for C, 26.4% for T and 16.8% for G, with a higher AT content of 56.4%. All 13 mitochondrial protein-coding genes share the start codon ATG, except for COI (GTG start codon). It is important to note that three of the 13 protein-coding genes are inferred to termination with an incomplete stop codon T– – (CO II and Cyt B) and TA– (CO III); seven of them share typical termination codon TAA (ND2, COI, ATPase 8, ATPase 6, ND3, ND4L and ND5); four of them share typical termination codon TAG (ND1, COIII, ND4 and ND6). Many cyprinid fishes also used such incomplete codon structure as a signal to halt the process of protein translation (Ai et al., 2013; Chen, 2013; Chen et al., 2014; Huang et al., 2012; Li et al., 2012; Tang et al., 2013; Tseng, 2013; Zhou et al., 2014). The longest one is ND5 gene (1833 bp) in all protein coding genes, whereas the shortest is ATPase 8 gene (165 bp). The CR (D-loop) is 1004 bp in length, and is located between the tRNAPro and tRNAPhe genes. The two ribosomal RNA genes, 12S rRNA gene (957 bp) and 16S rRNA gene (1690 bp), are located between tRNAPhe and tRNALeu(UUR) and separated by tRNAVal. We expect that the present result will elucidate the further phylogenetic approach among the related group in Cyprinidae as well as the phylogenetic approach of Sarcocheilichthys species in Eastern Asia.
930
I-S. Chen et al.
Mitochondrial DNA, 2015; 26(6): 929–931
Mitochondrial DNA 2015.26:929-931.
Table 1. Organization of the mitogenome in S. hainanensis.
Gene/element
From
To
Length
tRNA-Phe 12S rRNA tRNA-Val 16S rRNA tRNA-Leu 1 (UAA) ND1 tRNA-Ile tRNA-Gln tRNA-Met ND2 tRNA-Trp tRNA-Ala tRNA-Asn tRNA-Cys tRNA-Tyr CO 1 tRNA-Ser 1 (UGA) tRNA-Asp CO 2 tRNA-Lys ATP8 ATP6 CO 3 tRNA-Gly ND3 tRNA-Arg ND4L ND4 tRNA-His tRNA-Ser 2 (GCU) tRNA-Leu 2 (UAG) ND5 ND6 tRNA-Glu Cyt B tRNA-Thr tRNA-Pro Control region (D-loop)
1 70 1027 1099 2789 2866 3845 3915 3987 4056 5102 5176 5246 5350 5419 5491 7042 7116 7196 7887 7964 8122 8805 9589 9661 10,011 10,081 10,371 11,753 11822 11,892 11,965 13,794 14,316 14,389 15,530 15,602 15,671
69 1026 1098 2788 2864 3840 3916 3985 4055 5102 5173 5244 5318 5417 5489 7041 7112 7187 7886 7962 8128 8805 9589 9660 10,011 10,080 10,377 11,753 11,821 11890 11,964 13,797 14,315 14,384 15,529 15,601 15,670 16,674
69 957 72 1690 76 975 72 71 69 1047 72 69 73 68 71 1551 71 72 691 76 165 684 785 72 351 70 297 1383 69 69 73 1833 522 69 1141 72 69 1004
Start codon
Stop codon
Anticodon GAA TAC TAA
ATG
TAG GAT TTG CAT
ATG
TAA TCA TGC GTT GCA GTA
GTG
TAA TGA GTC
ATG
T– –
ATG ATG ATG
TAA TAA TA–
ATG
TAA
ATG ATG
TAA TAG
TTT
TCC TCG GTG GCT TAG ATG ATG
TAA TAG
ATG
T– –
TTC TGT TGG
Inetrgenic nucleotides*
Strand
0 0 0 0 1 4 2 1 0 1 2 1 31 1 1 0 3 8 0 1 7 1 1 0 1 0 7 1 0 1 0 4 0 4 0 0 0 0
H H H H H H H L H H H L L L L H L H H H H H H H H H H H H H H H L L H H L –
*Intergenic nucleotides: negative number indicates that adjacent genes overlap.
Declaration of interest The authors declare no competing materials in the preparation and execution of this manuscript. The authors are responsible for the content and writing of this paper. The corresponding author (ISC) wishes to thank for the grant support for aquatic biodiversity research from CMBB, NTOU. The current work was also supported from the Taiwan–Russia international project grants of National Science Council, Taipei, for the corresponding author.
References Ai W, Chen X, Xiang D, Chen S, Chen Y. (2013). Complete mitochondrial genome of Acrossocheilus wenchowensis (Cyprinidae, Barbinae). Mitochondrial DNA 24:249–51. Bleeker P. (1860). Conspectus systematis Cyprinorum. Natuurkundig Tijdschrift voor Nederlandsch Indie¨ 20:421–41. Chen IS. (2013). The complete mitochondrial genome of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae). Mitochondrial DNA 24:680–2. Chen IS, Han M, Wang CL, Shen CN. (2014). The complete mitochondrial genome of rainbow barbel Acrossocheilus barbodon (Nichols and Pope) (Teleostei, Cyprinidae, Barbinae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/19401736.2013.819495. Chen IS, Wu JH. (2014). The complete mitochondrial genome of variegated lizardfish Synodus variegatus (Perciformes, Synodontidae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/ 19401736.2013.775264.
Chiang TY, Chen IS, Lin HD, Chang WB, Ju YM. (2013). Complete mitochondrial genome of Sicyopterus japonicus (Perciformes, Gobiidae). Mitochondrial DNA 24:191–3. Han M, Wang CL, Shen CN, Chen IS. (2014). The complete mitochondrial genome of half-spined barbel Acrossocheilus hemispinus (Nichols) (Teleostei, Cyprinidae, Barbinae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/ 19401736.2013.815172. He S, Liang XF, Chu WY, Chen DX. (2012). Complete mitochondrial genome of the blind cave barbell Sinocyclocheilus furcodorsalis (Cypriniformes: Cyprinidae). Mitochondrial DNA 23: 429–31. Huang Y, Zhao GQ, Peng ZG. (2012). Mitochondrial genome of Onychostoma lini (Teleostei, Cypriniformes). Mitochondrial DNA 23:173–5. Li P, Xu D, Peng Z, Zhang Y. (2012). The complete mitochondrial genome of the spotted steed, Hemibarbus maculatus (Teleostei: Cypriniformes). Mitochondrial DNA 23:34–6. Miya M, Nishida M. (1999). Organization of the mitochondrial genome of a deep-sea fish, Gonostoma gracile (Teleostei: Stomiiformes): first example of transfer RNA gene arrangements in bony fish. Mar Biotechnol 1:416–26. Nichols JT, Pope CH. (1927). The fishes of Hainan. Bull Amer Mus Nat Hist 54:321–94. Tang WR, Lin HD, Su LW, Tang WQ, Wu CY, Yang JQ. (2013). The complete mitochondrial genome sequence of Sarcocheilichthys nigripinnis (Cypriniformes, Cyprinidae). Mitochondrial DNA 24: 478–80.
DOI: 10.3109/19401736.2013.863295
Mitochondrial DNA 2015.26:929-931.
Tseng DY. (2013). Complete mitochondrial genome of Onychostoma barbata (Cypriniformes, Cyprinidae). Mitochondrial DNA 24:120–2. Wang C, Chen Q, Lu G, Xu J, Yang Q, Li S. (2008). Complete mitochondrial genome of the grass carp (Teleostei: Cyprinidae: Gobioninae). Gene 424:96–101. Wang J, Li P, Peng Z. (2011). The complete mitochondrial genome of Chinese rare minnow, Gobiopterus rarus (Teleostei: Cypriniformes). Mitochondrial DNA 22:178–80.
Mitochondrial genome of S. hainanensis
931
Yang JQ, Cheng HL, Wu CY, Tsai KC, Chiang HC, Wang CW, Lin HD. (2012). Complete mitochondrial genome of Scaphiodonichthys acathopterus (Cypriniformes, Cyprinidae). Mitochondrial DNA 24: 108–10. Zhou ZH, Zhang HY, Wang GF, Zheng TL. (2014). Complete mitochondrial genome of Chinese lake gudgeon Sarcocheilichthys sinensis (Cypriniformes, Cyprinidae). Mitochondrial DNA. [Epub ahead of print]. DOI: doi:10.3109/19401736.2013.815176.
ISSN: 1940-1736 (Print) 1940-1744 (Online) Journal homepage: http://www.tandfonline.com/loi/imdn20
The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae) I-Shiung Chen, Zong-Han Wen, Yi-Ta Shao & Chiao-Ru Liao To cite this article: I-Shiung Chen, Zong-Han Wen, Yi-Ta Shao & Chiao-Ru Liao (2015) The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae), Mitochondrial DNA, 26:6, 929-931, DOI: 10.3109/19401736.2013.863295 To link to this article: http://dx.doi.org/10.3109/19401736.2013.863295
Published online: 10 Jan 2014.
Submit your article to this journal
Article views: 16
View related articles
View Crossmark data
Citing articles: 7 View citing articles
Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=imdn20 Download by: [York University Libraries]
Date: 06 November 2015, At: 21:33
http://informahealthcare.com/mdn ISSN: 1940-1736 (print), 1940-1744 (electronic) Mitochondrial DNA, 2015; 26(6): 929–931 ! 2014 Informa UK Ltd. DOI: 10.3109/19401736.2013.863295
MITOGENOME ANNOUNCEMENT
The complete mitochondrial genome of Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope (Cypriniformes: Cyprinidae) I-Shiung Chen1, Zong-Han Wen1, Yi-Ta Shao2, and Chiao-Ru Liao1 Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan, ROC and 2Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC
Mitochondrial DNA 2015.26:929-931.
1
Abstract
Keywords
In this study, the complete mitogenome sequence of cyprinid fish, Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope, 1927 (Cypriniformes: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,674 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 1004 bp length is located between tRNAPro and tRNAPhe. The overall base composition of S. hainanensis is 30.0% for A, 26.7% for C, 26.4% for T and 16.8% for G, with a slight AT bias of 56.4%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of cyprinid fishes.
Hainan island, mitogenome, Sarcocheilichthys hainanensis
The cyprinid fishes are highly diverse group in freshwater habitats of SE and E Asia. The freshwater cyrpinid Sarcocheilichthys Bleeker, 1860 is typical freshwater fish in moderate running waters in river basins from Japan, Korea to China southward to Vietnam. The Hainan gudgeon, Sarcocheilichthys hainanensis Nichols & Pope, 1927 may be endemic to the Hainan island, China. The specimen was collected from the Nandujiang river basin, Hainan. A fin tissue was sampled and preserved in 95% ethanol. Total DNA was extracted from the fish tissue using the High Pure DNA Extraction Kit (Roche Applied Sciences, Penzberg, Germany). The mitogenome of S. hainanensis was amplified in its entirety using a long PCR technique (Miya & Nishida, 1999) with 18 pairs of primers designed in accordance with equivalent mitochondrial genome sequences of over 30 species of Cypriniformes (Chen, 2013; Chen & Wu, 2014; Han et al., 2014; He et al., 2012; Huang et al., 2012; Wang et al., 2008, 2011; Yang et al., 2012). The complete mitochondrial genome of S. hainanensis was found to be similar to that of most other vertebrates (Chen, 2013; Chen & Wu, 2013; Chiang et al., 2013). It was amplified and sequenced to be 16,674 bp in size (GenBank accession number KF765805), including 13 typical vertebrate protein-coding genes,
Correspondence: I-S. Chen, Institute of Marine Biology, National Taiwan Ocean University, Keelung 202, Taiwan, ROC. Tel: 886 24622192 ext. 5305. Fax: 886 224633152. E-mail: [email protected]
History Received 29 October 2013 Accepted 3 November 2013 Published online 10 January 2014
22 transfer RNA genes, 2 ribosomal RNA genes and a control region (Table 1). All genes were encoded on H-strand with exception of one protein-coding gene (ND6) and eight tRNA genes (tRNAGln, tRNAAla, tRNAAsn, tRNACys, tRNATyr, tRNASer(UCN), tRNAGlu and tRNAPro). The overall base composition of S. hainanensis is 30.0% for A, 26.7% for C, 26.4% for T and 16.8% for G, with a higher AT content of 56.4%. All 13 mitochondrial protein-coding genes share the start codon ATG, except for COI (GTG start codon). It is important to note that three of the 13 protein-coding genes are inferred to termination with an incomplete stop codon T– – (CO II and Cyt B) and TA– (CO III); seven of them share typical termination codon TAA (ND2, COI, ATPase 8, ATPase 6, ND3, ND4L and ND5); four of them share typical termination codon TAG (ND1, COIII, ND4 and ND6). Many cyprinid fishes also used such incomplete codon structure as a signal to halt the process of protein translation (Ai et al., 2013; Chen, 2013; Chen et al., 2014; Huang et al., 2012; Li et al., 2012; Tang et al., 2013; Tseng, 2013; Zhou et al., 2014). The longest one is ND5 gene (1833 bp) in all protein coding genes, whereas the shortest is ATPase 8 gene (165 bp). The CR (D-loop) is 1004 bp in length, and is located between the tRNAPro and tRNAPhe genes. The two ribosomal RNA genes, 12S rRNA gene (957 bp) and 16S rRNA gene (1690 bp), are located between tRNAPhe and tRNALeu(UUR) and separated by tRNAVal. We expect that the present result will elucidate the further phylogenetic approach among the related group in Cyprinidae as well as the phylogenetic approach of Sarcocheilichthys species in Eastern Asia.
930
I-S. Chen et al.
Mitochondrial DNA, 2015; 26(6): 929–931
Mitochondrial DNA 2015.26:929-931.
Table 1. Organization of the mitogenome in S. hainanensis.
Gene/element
From
To
Length
tRNA-Phe 12S rRNA tRNA-Val 16S rRNA tRNA-Leu 1 (UAA) ND1 tRNA-Ile tRNA-Gln tRNA-Met ND2 tRNA-Trp tRNA-Ala tRNA-Asn tRNA-Cys tRNA-Tyr CO 1 tRNA-Ser 1 (UGA) tRNA-Asp CO 2 tRNA-Lys ATP8 ATP6 CO 3 tRNA-Gly ND3 tRNA-Arg ND4L ND4 tRNA-His tRNA-Ser 2 (GCU) tRNA-Leu 2 (UAG) ND5 ND6 tRNA-Glu Cyt B tRNA-Thr tRNA-Pro Control region (D-loop)
1 70 1027 1099 2789 2866 3845 3915 3987 4056 5102 5176 5246 5350 5419 5491 7042 7116 7196 7887 7964 8122 8805 9589 9661 10,011 10,081 10,371 11,753 11822 11,892 11,965 13,794 14,316 14,389 15,530 15,602 15,671
69 1026 1098 2788 2864 3840 3916 3985 4055 5102 5173 5244 5318 5417 5489 7041 7112 7187 7886 7962 8128 8805 9589 9660 10,011 10,080 10,377 11,753 11,821 11890 11,964 13,797 14,315 14,384 15,529 15,601 15,670 16,674
69 957 72 1690 76 975 72 71 69 1047 72 69 73 68 71 1551 71 72 691 76 165 684 785 72 351 70 297 1383 69 69 73 1833 522 69 1141 72 69 1004
Start codon
Stop codon
Anticodon GAA TAC TAA
ATG
TAG GAT TTG CAT
ATG
TAA TCA TGC GTT GCA GTA
GTG
TAA TGA GTC
ATG
T– –
ATG ATG ATG
TAA TAA TA–
ATG
TAA
ATG ATG
TAA TAG
TTT
TCC TCG GTG GCT TAG ATG ATG
TAA TAG
ATG
T– –
TTC TGT TGG
Inetrgenic nucleotides*
Strand
0 0 0 0 1 4 2 1 0 1 2 1 31 1 1 0 3 8 0 1 7 1 1 0 1 0 7 1 0 1 0 4 0 4 0 0 0 0
H H H H H H H L H H H L L L L H L H H H H H H H H H H H H H H H L L H H L –
*Intergenic nucleotides: negative number indicates that adjacent genes overlap.
Declaration of interest The authors declare no competing materials in the preparation and execution of this manuscript. The authors are responsible for the content and writing of this paper. The corresponding author (ISC) wishes to thank for the grant support for aquatic biodiversity research from CMBB, NTOU. The current work was also supported from the Taiwan–Russia international project grants of National Science Council, Taipei, for the corresponding author.
References Ai W, Chen X, Xiang D, Chen S, Chen Y. (2013). Complete mitochondrial genome of Acrossocheilus wenchowensis (Cyprinidae, Barbinae). Mitochondrial DNA 24:249–51. Bleeker P. (1860). Conspectus systematis Cyprinorum. Natuurkundig Tijdschrift voor Nederlandsch Indie¨ 20:421–41. Chen IS. (2013). The complete mitochondrial genome of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae). Mitochondrial DNA 24:680–2. Chen IS, Han M, Wang CL, Shen CN. (2014). The complete mitochondrial genome of rainbow barbel Acrossocheilus barbodon (Nichols and Pope) (Teleostei, Cyprinidae, Barbinae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/19401736.2013.819495. Chen IS, Wu JH. (2014). The complete mitochondrial genome of variegated lizardfish Synodus variegatus (Perciformes, Synodontidae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/ 19401736.2013.775264.
Chiang TY, Chen IS, Lin HD, Chang WB, Ju YM. (2013). Complete mitochondrial genome of Sicyopterus japonicus (Perciformes, Gobiidae). Mitochondrial DNA 24:191–3. Han M, Wang CL, Shen CN, Chen IS. (2014). The complete mitochondrial genome of half-spined barbel Acrossocheilus hemispinus (Nichols) (Teleostei, Cyprinidae, Barbinae). Mitochondrial DNA. [Epub ahead of print]. DOI: 10.3109/ 19401736.2013.815172. He S, Liang XF, Chu WY, Chen DX. (2012). Complete mitochondrial genome of the blind cave barbell Sinocyclocheilus furcodorsalis (Cypriniformes: Cyprinidae). Mitochondrial DNA 23: 429–31. Huang Y, Zhao GQ, Peng ZG. (2012). Mitochondrial genome of Onychostoma lini (Teleostei, Cypriniformes). Mitochondrial DNA 23:173–5. Li P, Xu D, Peng Z, Zhang Y. (2012). The complete mitochondrial genome of the spotted steed, Hemibarbus maculatus (Teleostei: Cypriniformes). Mitochondrial DNA 23:34–6. Miya M, Nishida M. (1999). Organization of the mitochondrial genome of a deep-sea fish, Gonostoma gracile (Teleostei: Stomiiformes): first example of transfer RNA gene arrangements in bony fish. Mar Biotechnol 1:416–26. Nichols JT, Pope CH. (1927). The fishes of Hainan. Bull Amer Mus Nat Hist 54:321–94. Tang WR, Lin HD, Su LW, Tang WQ, Wu CY, Yang JQ. (2013). The complete mitochondrial genome sequence of Sarcocheilichthys nigripinnis (Cypriniformes, Cyprinidae). Mitochondrial DNA 24: 478–80.
DOI: 10.3109/19401736.2013.863295
Mitochondrial DNA 2015.26:929-931.
Tseng DY. (2013). Complete mitochondrial genome of Onychostoma barbata (Cypriniformes, Cyprinidae). Mitochondrial DNA 24:120–2. Wang C, Chen Q, Lu G, Xu J, Yang Q, Li S. (2008). Complete mitochondrial genome of the grass carp (Teleostei: Cyprinidae: Gobioninae). Gene 424:96–101. Wang J, Li P, Peng Z. (2011). The complete mitochondrial genome of Chinese rare minnow, Gobiopterus rarus (Teleostei: Cypriniformes). Mitochondrial DNA 22:178–80.
Mitochondrial genome of S. hainanensis
931
Yang JQ, Cheng HL, Wu CY, Tsai KC, Chiang HC, Wang CW, Lin HD. (2012). Complete mitochondrial genome of Scaphiodonichthys acathopterus (Cypriniformes, Cyprinidae). Mitochondrial DNA 24: 108–10. Zhou ZH, Zhang HY, Wang GF, Zheng TL. (2014). Complete mitochondrial genome of Chinese lake gudgeon Sarcocheilichthys sinensis (Cypriniformes, Cyprinidae). Mitochondrial DNA. [Epub ahead of print]. DOI: doi:10.3109/19401736.2013.815176.
