The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours Tiberiu Popescu, Iuliana Nenu, Mihaela D. Aldea, Diana Olteanu, Dan Gheban, Corina Tatomir, Pompei Bolfa, Adriana Muresan, Rodica M. Ion, Adriana G. Filip PII: DOI: Reference:
S0024-3205(14)00795-4 doi: 10.1016/j.lfs.2014.09.022 LFS 14159
To appear in:
Life Sciences
Received date: Accepted date:
23 June 2014 23 September 2014
Please cite this article as: Popescu Tiberiu, Nenu Iuliana, Aldea Mihaela D., Olteanu Diana, Gheban Dan, Tatomir Corina, Bolfa Pompei, Muresan Adriana, Ion Rodica M., Filip Adriana G., The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours, Life Sciences (2014), doi: 10.1016/j.lfs.2014.09.022
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours Tiberiu Popescu1*, Iuliana Nenu1*, Mihaela D. Aldea1, Diana Olteanu1, Dan Gheban2,
Department of Physiology,”Iuliu Hatieganu” University of Medicine and Pharmacy, 1 Clinicilor
RI
1
PT
Corina Tatomir3, Pompei Bolfa4, 5, Adriana Muresan1, Rodica M. Ion6, Adriana G. Filip1#
Street, 400006 Cluj-Napoca, Romania
Department of Morphopathology,”Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5
SC
2
Clinicilor Street, 400006 Cluj-Napoca, Romania
Departments of Radiobiology and Tumour Biology, Oncology Institute ”Prof. Dr. I. Chiricuta”,
NU
3
34-36 Republicii Street, 400015 Cluj-Napoca, Romania
Pathology Department, University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj-
MA
4
Napoca, Romania 5
Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, St.
National Research &Development Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul
TE
6
D
Kitts, West Indies
#
AC CE P
Independentei, 060021 Bucharest, Romania
corresponding author
Adriana G. Filip
Department of Physiology
1-3 Clinicilor Street, Cluj-Napoca, Romania [email protected]
fax: +40-264-597257; Tel: +40-745-268704 * These authors contributed equally to this work
1
ACCEPTED MANUSCRIPT Abstract Aims: The study investigated the effects of the combined treatment Parecoxib (Pcox) and 5,10,15,20-tetra-sulphonato-phenyl-porphyrin(TSPP)-mediated photodynamic therapy on Walker 256 carcinosarcoma.
PT
Main methods: Five groups of male Wistar rats were used: the control group, treated with
RI
TSPP, group 2, irradiated 24hrs thereafter, group 3, treated with Pcox and irradiated 24hrs thereafter, groups 4 and 5 treated with combined therapies, TSPP and Pcox before irradiation, and Pcox 24hrs
SC
after TSPP and irradiation respectively. Tumour inflammation, growth and non-growth factors, apoptotis/necrosis rate and oxidative/nitrosative stress markers were investigated.
NU
Key findings: Malondialdehyde levels and cyclooxygenase (COX)-2 expression increased significantly in the group treated with Pcox after TSPP-PDT when compared with TSPP+IR group
MA
(p
S0024-3205(14)00795-4 doi: 10.1016/j.lfs.2014.09.022 LFS 14159
To appear in:
Life Sciences
Received date: Accepted date:
23 June 2014 23 September 2014
Please cite this article as: Popescu Tiberiu, Nenu Iuliana, Aldea Mihaela D., Olteanu Diana, Gheban Dan, Tatomir Corina, Bolfa Pompei, Muresan Adriana, Ion Rodica M., Filip Adriana G., The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours, Life Sciences (2014), doi: 10.1016/j.lfs.2014.09.022
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT The effect of TSPP-mediated photodynamic therapy and Parecoxib in experimental tumours Tiberiu Popescu1*, Iuliana Nenu1*, Mihaela D. Aldea1, Diana Olteanu1, Dan Gheban2,
Department of Physiology,”Iuliu Hatieganu” University of Medicine and Pharmacy, 1 Clinicilor
RI
1
PT
Corina Tatomir3, Pompei Bolfa4, 5, Adriana Muresan1, Rodica M. Ion6, Adriana G. Filip1#
Street, 400006 Cluj-Napoca, Romania
Department of Morphopathology,”Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5
SC
2
Clinicilor Street, 400006 Cluj-Napoca, Romania
Departments of Radiobiology and Tumour Biology, Oncology Institute ”Prof. Dr. I. Chiricuta”,
NU
3
34-36 Republicii Street, 400015 Cluj-Napoca, Romania
Pathology Department, University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj-
MA
4
Napoca, Romania 5
Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, St.
National Research &Development Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul
TE
6
D
Kitts, West Indies
#
AC CE P
Independentei, 060021 Bucharest, Romania
corresponding author
Adriana G. Filip
Department of Physiology
1-3 Clinicilor Street, Cluj-Napoca, Romania [email protected]
fax: +40-264-597257; Tel: +40-745-268704 * These authors contributed equally to this work
1
ACCEPTED MANUSCRIPT Abstract Aims: The study investigated the effects of the combined treatment Parecoxib (Pcox) and 5,10,15,20-tetra-sulphonato-phenyl-porphyrin(TSPP)-mediated photodynamic therapy on Walker 256 carcinosarcoma.
PT
Main methods: Five groups of male Wistar rats were used: the control group, treated with
RI
TSPP, group 2, irradiated 24hrs thereafter, group 3, treated with Pcox and irradiated 24hrs thereafter, groups 4 and 5 treated with combined therapies, TSPP and Pcox before irradiation, and Pcox 24hrs
SC
after TSPP and irradiation respectively. Tumour inflammation, growth and non-growth factors, apoptotis/necrosis rate and oxidative/nitrosative stress markers were investigated.
NU
Key findings: Malondialdehyde levels and cyclooxygenase (COX)-2 expression increased significantly in the group treated with Pcox after TSPP-PDT when compared with TSPP+IR group
MA
(p
