Author's Accepted Manuscript
The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion Hayriye Erman, Ibrahim Guner, Muhittin Onur Yaman, Duygu Derya Uzun, Remise Gelisgen, Ugur Aksu, Nermin Yelmen, Gulderen Sahin, Hafize Uzun www.elsevier.com/locate/ejphar
PII: DOI: Reference:
S0014-2999(15)00027-8 http://dx.doi.org/10.1016/j.ejphar.2015.01.007 EJP69701
To appear in:
European Journal of Pharmacology
Received date: 16 May 2014 Revised date: 19 December 2014 Accepted date: 5 January 2015 Cite this article as: Hayriye Erman, Ibrahim Guner, Muhittin Onur Yaman, Duygu Derya Uzun, Remise Gelisgen, Ugur Aksu, Nermin Yelmen, Gulderen Sahin, Hafize Uzun, The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion, European Journal of Pharmacology, http://dx.doi.org/10.1016/j.ejphar.2015.01.007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion Hayriye Erman1, Ibrahim Guner2, Muhittin Onur Yaman2, Duygu Derya Uzun3, Remise Gelisgen1, Ugur Aksu4, Nermin Yelmen2, Gulderen Sahin 2, Hafize Uzun1 1
Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey. 2 Istanbul University, Cerrahpasa Medical Faculty, Department of Physiology, Istanbul, Turkey. 3 Istanbul University, Istanbul Medical Faculty, Student, Istanbul, Turkey. 4 Science Faculty, Department of Biology, Istanbul University, Istanbul, Turkey.
Corresponding Address: Prof.Dr.Hafize Uzun Istanbul University, Cerrahpasa Medical Faculty Department of Biochemistry 34303, Cerrahpasa- Istanbul / Turkey e-mail: [email protected] Phone: +90 212 414 30 56 Fax: +90 212 633 29 87
ABSTRACT Oxidative stress and reperfusion injury may develop in different ischemia-reperfusion (IR) models. Growing evidence links altered lipid protein redox-homeostasis with IR. The effect of fluoxetine (FLX;N-methyl-3-[4-(trifluoromethyl) phenoxy] benzenepropanamine), on the lipid protein redox-homeostasis mechanisms in the rats exposed to aortic IR is unclear. We aimed to investigate the effects of FLX on circulating protein oxidation and lipid peroxidation parameters, such as ischemia modified albumin (IMA), lipid hydroperoxide (LOOH), prooxidant antioxidant balance (PAB), erythrocyte glutathione (GSH), CuZn-superoxide dismutase (CuZn-SOD), ferric reducing antioxidant power (FRAP), as potential IR biomarkers. Wistar rats were randomised into three groups (n=7/group): 1) Control (sham laparotomy); 2) IR without FLX, (60 min ischemia and 120 min reperfusion); 3) IR with FLX (FLX+IR) (FLX 20 mg/kg/day, i.p. for three day before surgery). All of the aforementioned parameters (IMA, LOOH, PAB, GSH, CuZn-SOD, FRAP) were measured spectrophotometrically. IMA, LOOH, and PAB levels in IR group were significantly higher than the control (P
The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion Hayriye Erman, Ibrahim Guner, Muhittin Onur Yaman, Duygu Derya Uzun, Remise Gelisgen, Ugur Aksu, Nermin Yelmen, Gulderen Sahin, Hafize Uzun www.elsevier.com/locate/ejphar
PII: DOI: Reference:
S0014-2999(15)00027-8 http://dx.doi.org/10.1016/j.ejphar.2015.01.007 EJP69701
To appear in:
European Journal of Pharmacology
Received date: 16 May 2014 Revised date: 19 December 2014 Accepted date: 5 January 2015 Cite this article as: Hayriye Erman, Ibrahim Guner, Muhittin Onur Yaman, Duygu Derya Uzun, Remise Gelisgen, Ugur Aksu, Nermin Yelmen, Gulderen Sahin, Hafize Uzun, The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion, European Journal of Pharmacology, http://dx.doi.org/10.1016/j.ejphar.2015.01.007 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion Hayriye Erman1, Ibrahim Guner2, Muhittin Onur Yaman2, Duygu Derya Uzun3, Remise Gelisgen1, Ugur Aksu4, Nermin Yelmen2, Gulderen Sahin 2, Hafize Uzun1 1
Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey. 2 Istanbul University, Cerrahpasa Medical Faculty, Department of Physiology, Istanbul, Turkey. 3 Istanbul University, Istanbul Medical Faculty, Student, Istanbul, Turkey. 4 Science Faculty, Department of Biology, Istanbul University, Istanbul, Turkey.
Corresponding Address: Prof.Dr.Hafize Uzun Istanbul University, Cerrahpasa Medical Faculty Department of Biochemistry 34303, Cerrahpasa- Istanbul / Turkey e-mail: [email protected] Phone: +90 212 414 30 56 Fax: +90 212 633 29 87
ABSTRACT Oxidative stress and reperfusion injury may develop in different ischemia-reperfusion (IR) models. Growing evidence links altered lipid protein redox-homeostasis with IR. The effect of fluoxetine (FLX;N-methyl-3-[4-(trifluoromethyl) phenoxy] benzenepropanamine), on the lipid protein redox-homeostasis mechanisms in the rats exposed to aortic IR is unclear. We aimed to investigate the effects of FLX on circulating protein oxidation and lipid peroxidation parameters, such as ischemia modified albumin (IMA), lipid hydroperoxide (LOOH), prooxidant antioxidant balance (PAB), erythrocyte glutathione (GSH), CuZn-superoxide dismutase (CuZn-SOD), ferric reducing antioxidant power (FRAP), as potential IR biomarkers. Wistar rats were randomised into three groups (n=7/group): 1) Control (sham laparotomy); 2) IR without FLX, (60 min ischemia and 120 min reperfusion); 3) IR with FLX (FLX+IR) (FLX 20 mg/kg/day, i.p. for three day before surgery). All of the aforementioned parameters (IMA, LOOH, PAB, GSH, CuZn-SOD, FRAP) were measured spectrophotometrically. IMA, LOOH, and PAB levels in IR group were significantly higher than the control (P
