Cell, Vol. 66, 629-640,
March 6, 1992, Copyright
0 1992 by Cell Press
The Golgi Complex: In Vitro Veritas? Ira Mellman’ and Kai Simonst *Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510 tCell Biology Program European Molecular Biology Laboratory 1 Meyerhofstrasse D-6900 Heidelberg IFederal Republic of Germany
Understanding
thestructure
and function of theGolgi
comprobllems in cell biology. The last several years have turned out Ito be particularly exciting in this respect since they have Iyielded new insights and ideas at an increasingly rapid ipace. This period of advance has largely been due to the Idevelopment of powerful new biochemical, morphological, #and genetic approaches to unraveling the complexities of ‘this organelle. While much remains to be discovered, the Iproblem now is how to integrate this wealth of information. ‘To see if this is possible, we will first summarize how the lslolgi is commonly believed to work and then evaluate the lstrength of the evidence that underlies these views.
iplex has proved to be among the more challenging
Present View of the Golgi ‘The Golgi complex is essentially a carbohydrate factory. In
March 6, 1992, Copyright
0 1992 by Cell Press
The Golgi Complex: In Vitro Veritas? Ira Mellman’ and Kai Simonst *Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510 tCell Biology Program European Molecular Biology Laboratory 1 Meyerhofstrasse D-6900 Heidelberg IFederal Republic of Germany
Understanding
thestructure
and function of theGolgi
comprobllems in cell biology. The last several years have turned out Ito be particularly exciting in this respect since they have Iyielded new insights and ideas at an increasingly rapid ipace. This period of advance has largely been due to the Idevelopment of powerful new biochemical, morphological, #and genetic approaches to unraveling the complexities of ‘this organelle. While much remains to be discovered, the Iproblem now is how to integrate this wealth of information. ‘To see if this is possible, we will first summarize how the lslolgi is commonly believed to work and then evaluate the lstrength of the evidence that underlies these views.
iplex has proved to be among the more challenging
Present View of the Golgi ‘The Golgi complex is essentially a carbohydrate factory. In
