Original Article
The role of speckle tracking echocardiography in assessment of lipopolysaccharide-induced myocardial dysfunction in mice Ming Chu1, Yao Gao1, Yanjuan Zhang2, Bin Zhou2,3, Bingruo Wu2,3, Jing Yao2, Di Xu1 1
Department of Geriatrics, 2Department of Cardiology, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China;
3
Departments of Genetics, Pediatrics and Medicine (Cardiology), Albert Einstein College of Medicine of Yeshiva University, New York, NY, USA
Contributions: (I) Conception and design: M Chu, J Yao, D Xu; (II) Administrative support: B Wu, B Zhou; (III) Provision of study materials or patients: Y Gao, Y Zhang; (IV) Collection and assembly of data: Y Gao, Y Zhang; (V) Data analysis and interpretation: M Chu, Y Gao; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Di Xu, MD, PhD. Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. Email: [email protected]; Jing Yao, MD, PhD. Department of Cardiology, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. Email: [email protected].
Background: Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. Methods: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h ( n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. Results: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (−14.65%±3.00% to −8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P
The role of speckle tracking echocardiography in assessment of lipopolysaccharide-induced myocardial dysfunction in mice Ming Chu1, Yao Gao1, Yanjuan Zhang2, Bin Zhou2,3, Bingruo Wu2,3, Jing Yao2, Di Xu1 1
Department of Geriatrics, 2Department of Cardiology, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China;
3
Departments of Genetics, Pediatrics and Medicine (Cardiology), Albert Einstein College of Medicine of Yeshiva University, New York, NY, USA
Contributions: (I) Conception and design: M Chu, J Yao, D Xu; (II) Administrative support: B Wu, B Zhou; (III) Provision of study materials or patients: Y Gao, Y Zhang; (IV) Collection and assembly of data: Y Gao, Y Zhang; (V) Data analysis and interpretation: M Chu, Y Gao; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Di Xu, MD, PhD. Department of Geriatrics, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. Email: [email protected]; Jing Yao, MD, PhD. Department of Cardiology, First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, China. Email: [email protected].
Background: Sepsis-induced myocardial dysfunction is a common and severe complication of septic shock. Conventional echocardiography often fails to reveal myocardial depression in severe sepsis due to hemodynamic changes; in contrast, decline of strain measurements by speckle tracking echocardiography (STE) may indicate impaired cardiac function. This study investigates the role of STE in detecting lipopolysaccharide (LPS)-induced cardiac dysfunction with mouse models. Methods: We evaluated cardiac function in 20 mice at baseline, 6 h (n=10) and 20 h ( n=10) after LPS injection to monitor the development of heart failure induced by severe sepsis using 2-D and M-mode echocardiography. Ejection fraction (EF) and fractional shortening (FS) were measured with standard M-mode tracings, whereas circumferential and radial strain was derived from STE. Serum biochemical and cardiac histopathological examinations were performed to determine sepsis-induced myocardial injury. Results: Left ventricular (LV) myocardial function was significantly reduced at 6 h after LPS treatment assessed by circumferential strain (−14.65%±3.00% to −8.48%±1.72%, P=0.006), whereas there were no significant differences between 6 and 20 h group. Conversely, EF and FS were significantly increased at 20 h when comparing to 6 h (P
