Medical Hypotheses
7
L
I
Me&catffyporheses(1990) 33.173-174 Cl LongmanGroupUK Ltd 1990
0306~9877/90/003~-0173/%10.00
The Synergism of y-Interferon and Tumor Factor in Whole Body Hyperthermia
Necrosis
P. ROSEN Hasbrouck
Laboratory,
University
of Massachusetts,
Amherst,
Massachusetts
01003,
USA
Abstract - It is suggested that y-interferon (y-IFN) can be added to tumor necrosis factor (TNF) to improve therapy in whole body hyperthermia (WBH). The amount of r-HTNF is about 3.5 x 106 units per week with about 3.5 x 104 units of rH y-INF intravenously. The body temperature should be 40°C for one half hour. An i.p. injection of secobarbital (24 mglkg) should be given as well.
Introduction Previously (l), I suggested the use of 3.5 x lo6 units of r-HTNF with whole body hyperthermia of 40°C for one half hour as a cancer therapy. This is based on (2). However, the tumors in (2) are subdermal. I am interested in eliminating metastases after sugery instead of chemotherapy.
hyperthermia can minimize the toxicity of TNF. The use of TNF in resistant cells ME- 180neo(5) can cause growth of tumor cells as well as have little effect. This depends on the number of units of r-H TNF used. If one uses rH y-TNF of approximately 10-Zof the units of r-H TNF, the resistant cells are completely destroyed. Conclusion
Side effects of tumor necrosis factor
According to Old (3) TNF can be toxic. Also, TNF may be ineffective against resistant cells. In addition (4), TNF can even enhance the growth of tumor cells.
For metastatic cancer, after surgery one should be able to control or cure patients with body hyperthermia of 40°C for one half hour upon adding 3.5 x 106 units of r-H TNF with 3.5 x lo+4 units of rH y- TNF per week i.v. In addition 24 mg/kg of secobarbital is used i.p. The exact protocol must be obtained experimentally.
Synergism of TNF and y-INF It has been suggested by Old (3) that INF can
References
aid TNF synergistically. The effect of whole body Date received Date accepted
1. Rosen P. Successful whole body hyperthermia
18 July 1989 19 February 1990
173
for cancer.
174 Med. Hyp. 29: 45, 1989. 2. Natabe N, Njitsu Y, Umeno H, Sone H, Neda H, Yamauchi N, Malda M and Urushizaki J. Synergistic cytotoxic and anti-tumor effects of recombinant human tumor necrosis factor and hyperthermia. Cancer Research 48: 650, 1988. 3. Old LJ. Perspecitve: tumor necrosis factor (TNF) Science 230: 630, 1985.
MEDICAL HYPOTHESES
4. Brenner DA, O’Hara M, Angel P, Chojkier M and Karin M. Prolonged activation of jun and collagenase genes by tumor necrosis factor- c. Nature 337: 661, 1989. 5. Lewis GD, Aggarwal BB, Eessalu TE, Sugarman BJ and Sheperd HM. Modulation of the growth of transformed cells by humor tumor necrosis factor- @and interferon - y. Cancer Research 47: 5382, 1987
7
L
I
Me&catffyporheses(1990) 33.173-174 Cl LongmanGroupUK Ltd 1990
0306~9877/90/003~-0173/%10.00
The Synergism of y-Interferon and Tumor Factor in Whole Body Hyperthermia
Necrosis
P. ROSEN Hasbrouck
Laboratory,
University
of Massachusetts,
Amherst,
Massachusetts
01003,
USA
Abstract - It is suggested that y-interferon (y-IFN) can be added to tumor necrosis factor (TNF) to improve therapy in whole body hyperthermia (WBH). The amount of r-HTNF is about 3.5 x 106 units per week with about 3.5 x 104 units of rH y-INF intravenously. The body temperature should be 40°C for one half hour. An i.p. injection of secobarbital (24 mglkg) should be given as well.
Introduction Previously (l), I suggested the use of 3.5 x lo6 units of r-HTNF with whole body hyperthermia of 40°C for one half hour as a cancer therapy. This is based on (2). However, the tumors in (2) are subdermal. I am interested in eliminating metastases after sugery instead of chemotherapy.
hyperthermia can minimize the toxicity of TNF. The use of TNF in resistant cells ME- 180neo(5) can cause growth of tumor cells as well as have little effect. This depends on the number of units of r-H TNF used. If one uses rH y-TNF of approximately 10-Zof the units of r-H TNF, the resistant cells are completely destroyed. Conclusion
Side effects of tumor necrosis factor
According to Old (3) TNF can be toxic. Also, TNF may be ineffective against resistant cells. In addition (4), TNF can even enhance the growth of tumor cells.
For metastatic cancer, after surgery one should be able to control or cure patients with body hyperthermia of 40°C for one half hour upon adding 3.5 x 106 units of r-H TNF with 3.5 x lo+4 units of rH y- TNF per week i.v. In addition 24 mg/kg of secobarbital is used i.p. The exact protocol must be obtained experimentally.
Synergism of TNF and y-INF It has been suggested by Old (3) that INF can
References
aid TNF synergistically. The effect of whole body Date received Date accepted
1. Rosen P. Successful whole body hyperthermia
18 July 1989 19 February 1990
173
for cancer.
174 Med. Hyp. 29: 45, 1989. 2. Natabe N, Njitsu Y, Umeno H, Sone H, Neda H, Yamauchi N, Malda M and Urushizaki J. Synergistic cytotoxic and anti-tumor effects of recombinant human tumor necrosis factor and hyperthermia. Cancer Research 48: 650, 1988. 3. Old LJ. Perspecitve: tumor necrosis factor (TNF) Science 230: 630, 1985.
MEDICAL HYPOTHESES
4. Brenner DA, O’Hara M, Angel P, Chojkier M and Karin M. Prolonged activation of jun and collagenase genes by tumor necrosis factor- c. Nature 337: 661, 1989. 5. Lewis GD, Aggarwal BB, Eessalu TE, Sugarman BJ and Sheperd HM. Modulation of the growth of transformed cells by humor tumor necrosis factor- @and interferon - y. Cancer Research 47: 5382, 1987
