Amino Acids DOI 10.1007/s00726-015-2019-9
ORIGINAL ARTICLE
Three‑minute method for amino acid analysis by UHPLC and high‑resolution quadrupole orbitrap mass spectrometry Travis Nemkov1 · Angelo D’Alessandro1,2 · Kirk C. Hansen1,2
Received: 17 March 2015 / Accepted: 28 May 2015 © Springer-Verlag Wien 2015
Abstract Amino acid analysis is a powerful bioanalytical technique for many biomedical research endeavors, including cancer, emergency medicine, nutrition and neuroscience research. In the present study, we present a 3 min analytical method for underivatized amino acid analysis that employs ultra high-performance liquid chromatography and high-resolution quadrupole orbitrap mass spectrometry. This method has demonstrated linearity (mM to nM range), reproducibility (intra-day
ORIGINAL ARTICLE
Three‑minute method for amino acid analysis by UHPLC and high‑resolution quadrupole orbitrap mass spectrometry Travis Nemkov1 · Angelo D’Alessandro1,2 · Kirk C. Hansen1,2
Received: 17 March 2015 / Accepted: 28 May 2015 © Springer-Verlag Wien 2015
Abstract Amino acid analysis is a powerful bioanalytical technique for many biomedical research endeavors, including cancer, emergency medicine, nutrition and neuroscience research. In the present study, we present a 3 min analytical method for underivatized amino acid analysis that employs ultra high-performance liquid chromatography and high-resolution quadrupole orbitrap mass spectrometry. This method has demonstrated linearity (mM to nM range), reproducibility (intra-day
