protocol

Tracheal tissue engineering in rats Philipp Jungebluth1, Johannes C Haag1, Sebastian Sjöqvist1, Ylva Gustafsson1, Antonio Beltrán Rodríguez1, Costantino Del Gaudio2, Alessandra Bianco2, Ivar Dehnisch3, Per Uhlén3, Silvia Baiguera1, Greg Lemon1, Mei Ling Lim1 & Paolo Macchiarini1 1Advanced Center for Translational Regenerative Medicine, Department for Clinical Science, Intervention and Technology, Division of

Ear, Nose and Throat, Stockholm, Sweden. 2Department of Enterprise Engineering, University of Rome, Intrauniversity Consortium for Material Science and Technology (INSTM), Research Unit ‘Tor Vergata’, Rome, Italy. 3Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. Correspondence should be addressed to P.M. ([email protected]).

© 2014 Nature America, Inc. All rights reserved.

Published online 14 August 2014; doi:10.1038/nprot.2014.149

Tissue-engineered tracheal transplants have been successfully performed clinically. However, before becoming a routine clinical procedure, further preclinical studies are necessary to determine the underlying mechanisms of in situ tissue regeneration. Here we describe a protocol using a tissue engineering strategy and orthotopic transplantation of either natural decellularized donor tracheae or artificial electrospun nanofiber scaffolds into a rat model. The protocol includes details regarding how to assess the scaffolds’ biomechanical properties and cell viability before implantation. It is a reliable and reproducible model that can be used to investigate the crucial aspects and pathways of in situ tracheal tissue restoration and regeneration. The model can be established in

Tracheal tissue engineering in rats.

Tissue-engineered tracheal transplants have been successfully performed clinically. However, before becoming a routine clinical procedure, further pre...
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