Vol.
176,
No.
May
15,
1991
3, 1991
BIOCHEMICAL
AND
BIOPHYSICAL
COMMUNlCATfONS
1333-1337
Pages
TRANSCRlPTION
OF
RIBOSOMAL
DNA
INTEROENIC
AND REGENERATING I. D. Kalcheva. Institute
of
Cell
March
25,
SPACER
RAT
L. K. Karagyozov
Biology
and
Sciences, Received
RESEARCH
and
Sofia,
IN
NORMAL
LIVER A. A. Hadjiolov
Morphology,
1113
SEQUENCES
Bulgarian
Academy
of
Bulgaria
1991
In regenerating rat liver both the transcriptional activity of the intergenic spacer rDNA promotor and the steady-state abundance of spacer transcripts are increased about a-fold, as compared to normal liver. These changes are parallel to the observed 2.5-fold increase in regenerating liver of rRNA gene promotor activity and gene promotor transcripts abbundance. These results suggest that both gene and spacer rDNA promotors are subject to common regulatory mechanisms. Our results indicate also that the stability of spacer transcripts in regenerating liver is not significantly altered. 0 1991 Academic Press, Inc.
In
eukaryotes
tandemly
the
arrayed
repeating
and
polymerase
I
initiation
species
123,
several role
nor
regulatory In
the
regenerating the
total
liver
nuclear is
MATERIALS Isolation Partial Wistar
in
normal
on
gene
gene
RNA
from
also
unchanged.
that
the
level
the
cell
type
in rRNA
Recently, the
Neither IUS
RNA 10s
of
biological,
the transcription of
are spacer
XenOpus
C71
indicates
its
expression. the
levels rat
spacer
of'
liver.
rRNA We
remains liver
the
but
the
gene
report
transcription
ratio
increased,
[Il.
of
regenerating is
clustered precursor
within
C3-63.
regenerating and
are by
identified
compared
transcription
genes
sequences
rodents
rRNA we
and
both
spacer
experiments increase
in
quantitative
2,
at
systems
transcripts
The
reveal as
promotor
and
gene/spacer
promotor of
excess The
earlier
the
in
nuclear
steady-state
evaluation
proceeds
at
structural
is
presented
higher
than
that
degradation increase suggests
of of
gene
the
mechanisms.
and
RNA
as
spacer
C6,
153. is
suggested
in
that
by
line
factors the
with
at
the
gene/spacer 1336
the
two
the
.
run-on
homology
results
higher
abundance
by
at
the
normal,
promotor
of
findings
to
a-fold
gene
results
transcription
that
compared
times
of
show site
in
I.
regenerating,
Further,
2,
transcripts
protection
cell-free
spacer
liver.
spacer
fragments
earlier
Fig.
and
regenerating
Table
gene
in
and
lruclease
promotor
In
shown
of
from
gene
gene
Si
rDNA
the
identified
results
abundance
at
of
by
protected
site,
The
RNA
liver
starts
promotor 163.
abundance
regenerating
and
Mapping
the
abundance
sites ratio
c151. is
Vol.
176,
similar
No.
in
relative
and
stability
of of
alteration
under a
BIOCHEMICAL
regenerating
enhancement the
3, 1991
in
heat-shock
general
phenomenon
normal
spacer
rRNA the
stability
related
BIOPHYSICAL
rat
regenerating
of
spacer
Xenopus
to
changes
shows are
in
in
RESEARCH
liver
transcripts
synthesis
conditions
AND
liver.
2. 3. 4. 5. 6. 7. 8. 9. 10.
il.
12. 13. 14.
116,
synthesis
rates.
Hadjiolov, A. A. The Nucleolus and Ribosome Biogenesis Springer-Verlag, Wien - New York, pp.268 Reeder, R.H. (1989) Current Option in Cell Biol. 1, 466-474. Harrington,C.A., and Chikaraishi, D.M. (1987) Mol. Cell. Biol. 7_, 314-325. Tower, J., Henderson,S.L., Dougherty,K.M., We jksnora, P. J., and Sollner-Webb,B. Mol. Cell. Biol. 9, 1513-1525. Kuhn,A., and Grummt, I. Nucleic Acids Res. 9, 7345-7352. and Rothblum,L.I. (1988) Nucleic Acids Res. Yang-Yen,H.-F.,
i-4, 15. 16. 17.
5557.
Smith,D.S., Oriashi,E., Yang-Yen,H.-F., and Rothblum,L.I. (1990) Nucleic Labhart,P., and Reeder,R. (1987) 56-60. Parker,K.A., and Bond,U. (1989)
Xie,W-Q., Acids Res. Proc.Natl.Acad.Sci. Mol.
Cell.
1337
Biol.
Chen,C., 1677-1685. USA
l8,
9,
2500-2512.
in
the the
established
cells
REFERENCES 1.
in
Therefore,
transcripts
HeLa rRNA
changes involved
not
RNA
in
that
rat
and
COMMUNICATIONS
84,
171
is
not
176,
No.
May
15,
1991
3, 1991
BIOCHEMICAL
AND
BIOPHYSICAL
COMMUNlCATfONS
1333-1337
Pages
TRANSCRlPTION
OF
RIBOSOMAL
DNA
INTEROENIC
AND REGENERATING I. D. Kalcheva. Institute
of
Cell
March
25,
SPACER
RAT
L. K. Karagyozov
Biology
and
Sciences, Received
RESEARCH
and
Sofia,
IN
NORMAL
LIVER A. A. Hadjiolov
Morphology,
1113
SEQUENCES
Bulgarian
Academy
of
Bulgaria
1991
In regenerating rat liver both the transcriptional activity of the intergenic spacer rDNA promotor and the steady-state abundance of spacer transcripts are increased about a-fold, as compared to normal liver. These changes are parallel to the observed 2.5-fold increase in regenerating liver of rRNA gene promotor activity and gene promotor transcripts abbundance. These results suggest that both gene and spacer rDNA promotors are subject to common regulatory mechanisms. Our results indicate also that the stability of spacer transcripts in regenerating liver is not significantly altered. 0 1991 Academic Press, Inc.
In
eukaryotes
tandemly
the
arrayed
repeating
and
polymerase
I
initiation
species
123,
several role
nor
regulatory In
the
regenerating the
total
liver
nuclear is
MATERIALS Isolation Partial Wistar
in
normal
on
gene
gene
RNA
from
also
unchanged.
that
the
level
the
cell
type
in rRNA
Recently, the
Neither IUS
RNA 10s
of
biological,
the transcription of
are spacer
XenOpus
C71
indicates
its
expression. the
levels rat
spacer
of'
liver.
rRNA We
remains liver
the
but
the
gene
report
transcription
ratio
increased,
[Il.
of
regenerating is
clustered precursor
within
C3-63.
regenerating and
are by
identified
compared
transcription
genes
sequences
rodents
rRNA we
and
both
spacer
experiments increase
in
quantitative
2,
at
systems
transcripts
The
reveal as
promotor
and
gene/spacer
promotor of
excess The
earlier
the
in
nuclear
steady-state
evaluation
proceeds
at
structural
is
presented
higher
than
that
degradation increase suggests
of of
gene
the
mechanisms.
and
RNA
as
spacer
C6,
153. is
suggested
in
that
by
line
factors the
with
at
the
gene/spacer 1336
the
two
the
.
run-on
homology
results
higher
abundance
by
at
the
normal,
promotor
of
findings
to
a-fold
gene
results
transcription
that
compared
times
of
show site
in
I.
regenerating,
Further,
2,
transcripts
protection
cell-free
spacer
liver.
spacer
fragments
earlier
Fig.
and
regenerating
Table
gene
in
and
lruclease
promotor
In
shown
of
from
gene
gene
Si
rDNA
the
identified
results
abundance
at
of
by
protected
site,
The
RNA
liver
starts
promotor 163.
abundance
regenerating
and
Mapping
the
abundance
sites ratio
c151. is
Vol.
176,
similar
No.
in
relative
and
stability
of of
alteration
under a
BIOCHEMICAL
regenerating
enhancement the
3, 1991
in
heat-shock
general
phenomenon
normal
spacer
rRNA the
stability
related
BIOPHYSICAL
rat
regenerating
of
spacer
Xenopus
to
changes
shows are
in
in
RESEARCH
liver
transcripts
synthesis
conditions
AND
liver.
2. 3. 4. 5. 6. 7. 8. 9. 10.
il.
12. 13. 14.
116,
synthesis
rates.
Hadjiolov, A. A. The Nucleolus and Ribosome Biogenesis Springer-Verlag, Wien - New York, pp.268 Reeder, R.H. (1989) Current Option in Cell Biol. 1, 466-474. Harrington,C.A., and Chikaraishi, D.M. (1987) Mol. Cell. Biol. 7_, 314-325. Tower, J., Henderson,S.L., Dougherty,K.M., We jksnora, P. J., and Sollner-Webb,B. Mol. Cell. Biol. 9, 1513-1525. Kuhn,A., and Grummt, I. Nucleic Acids Res. 9, 7345-7352. and Rothblum,L.I. (1988) Nucleic Acids Res. Yang-Yen,H.-F.,
i-4, 15. 16. 17.
5557.
Smith,D.S., Oriashi,E., Yang-Yen,H.-F., and Rothblum,L.I. (1990) Nucleic Labhart,P., and Reeder,R. (1987) 56-60. Parker,K.A., and Bond,U. (1989)
Xie,W-Q., Acids Res. Proc.Natl.Acad.Sci. Mol.
Cell.
1337
Biol.
Chen,C., 1677-1685. USA
l8,
9,
2500-2512.
in
the the
established
cells
REFERENCES 1.
in
Therefore,
transcripts
HeLa rRNA
changes involved
not
RNA
in
that
rat
and
COMMUNICATIONS
84,
171
is
not
