Am J Transl Res 2016;8(12):5706-5714 www.ajtr.org /ISSN:1943-8141/AJTR0033508
Original Article Treatment of de-peritonealized intestine with 4DryField® PH prevents adhesions between non-resorbable intra-peritoneal hernia mesh and bowel Markus Winny1, Lavinia Maegel2, Leonie Victoria Grethe1, Danny Jonigk2, Paul Borchert2, Alexander Kaltenborn3,4, Harald Schrem1,3, Juergen Klempnauer1, Daniel Poehnert1 Department of General, Visceral and Transplant Surgery, Hannover Medical School, Germany; 2Institute for Pathology, Hannover Medical School, Germany; 3Core Facility Quality Management and Health Technology Assessment in Transplantation, Integrated Research and Treatment Centre-Transplantation (IFB-Tx), Hannover Medical School, Germany; 4Department of Trauma and Orthopaedic Surgery, Federal Armed Forces Hospital Westerstede, Westerstede, Germany 1
Received June 9, 2016; Accepted August 16, 2016; Epub December 15, 2016; Published December 30, 2016 Abstract: Background: Intraperitoneal onlay meshes (IPOM) can be associated with intestine-to-mesh adhesion formation, implementing risks like pain, enterocutaneous fistula, infection, and female infertility. This study investigates, whether a treatment of impaired intestinum with the anti-adhesive and hemostyptic agent 4DryField® PH prevents adhesion formation. Methods: In 20 male LEWIS rats uncoated polypropylene meshes were sewn to the inner abdominal wall and the cecum of the respective animal was de-peritonealized by peritoneal abrasion by a gauze swap, and meso-sutures ensured a constant contact of injured areas. Rats were treated with 4DryField® PH gel either premixed or applied as a powder with in-situ transformation (100 mg powder plus 0.4 ml 0.9% saline solution). One week postoperatively, the extent of intestine-to-mesh adhesions and the quality of mesh ingrowth were evaluated macroscopically by two independent investigators using two scoring systems. Furthermore, specimens were analysed microscopically. All data were compared with control animals without 4DryField® PH treatment and analysed statistically using student’s t-test. Results: Treatment of de-peritonealised cecum with 4DryField® PH significantly reduced intestine-to-mesh adhesions in both treatment groups as compared to controls without 4DryField® PH treatment (68% reduction with premixed gel, P
Original Article Treatment of de-peritonealized intestine with 4DryField® PH prevents adhesions between non-resorbable intra-peritoneal hernia mesh and bowel Markus Winny1, Lavinia Maegel2, Leonie Victoria Grethe1, Danny Jonigk2, Paul Borchert2, Alexander Kaltenborn3,4, Harald Schrem1,3, Juergen Klempnauer1, Daniel Poehnert1 Department of General, Visceral and Transplant Surgery, Hannover Medical School, Germany; 2Institute for Pathology, Hannover Medical School, Germany; 3Core Facility Quality Management and Health Technology Assessment in Transplantation, Integrated Research and Treatment Centre-Transplantation (IFB-Tx), Hannover Medical School, Germany; 4Department of Trauma and Orthopaedic Surgery, Federal Armed Forces Hospital Westerstede, Westerstede, Germany 1
Received June 9, 2016; Accepted August 16, 2016; Epub December 15, 2016; Published December 30, 2016 Abstract: Background: Intraperitoneal onlay meshes (IPOM) can be associated with intestine-to-mesh adhesion formation, implementing risks like pain, enterocutaneous fistula, infection, and female infertility. This study investigates, whether a treatment of impaired intestinum with the anti-adhesive and hemostyptic agent 4DryField® PH prevents adhesion formation. Methods: In 20 male LEWIS rats uncoated polypropylene meshes were sewn to the inner abdominal wall and the cecum of the respective animal was de-peritonealized by peritoneal abrasion by a gauze swap, and meso-sutures ensured a constant contact of injured areas. Rats were treated with 4DryField® PH gel either premixed or applied as a powder with in-situ transformation (100 mg powder plus 0.4 ml 0.9% saline solution). One week postoperatively, the extent of intestine-to-mesh adhesions and the quality of mesh ingrowth were evaluated macroscopically by two independent investigators using two scoring systems. Furthermore, specimens were analysed microscopically. All data were compared with control animals without 4DryField® PH treatment and analysed statistically using student’s t-test. Results: Treatment of de-peritonealised cecum with 4DryField® PH significantly reduced intestine-to-mesh adhesions in both treatment groups as compared to controls without 4DryField® PH treatment (68% reduction with premixed gel, P
