JCM Accepted Manuscript Posted Online 29 April 2015 J. Clin. Microbiol. doi:10.1128/JCM.00402-15 Copyright © 2015, American Society for Microbiology. All Rights Reserved.
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Two Rapidly Growing Mycobacterial Species Isolated from a Brain Abscess: First Whole Genome Sequence
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of Mycobacterium immunogenum and Mycobacterium llatzerense
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Alexander L. Greninger1,2,†, Charles Langelier3,†, Gail Cunningham1, Chris Keh3, Michael Melgar4, Charles Y.
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Chiu1,2,3 and Steve Miller1,2*
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1
8
2
9
3
10
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Department of Laboratory Medicine, University of California, San Francisco, CA, USA 94107
UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA, 914107 Department of Medicine, Division of Infectious Diseases, University of California, San Francisco Department of Internal Medicine, University of California San Francisco, San Francisco, California, USA
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†
These authors contributed equally to this work.
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*Corresponding author
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Department of Laboratory Medicine
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University of California San Francisco
17
180 Berry St., Suite 290
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San Francisco CA 94107
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Key words: Rapidly growing mycobacteria, brain abscess, whole genome sequencing
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Abstract:
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Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a
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case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M.
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immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods,
26
and whole-genome sequencing showed
1
Two Rapidly Growing Mycobacterial Species Isolated from a Brain Abscess: First Whole Genome Sequence
2
of Mycobacterium immunogenum and Mycobacterium llatzerense
3 4
Alexander L. Greninger1,2,†, Charles Langelier3,†, Gail Cunningham1, Chris Keh3, Michael Melgar4, Charles Y.
5
Chiu1,2,3 and Steve Miller1,2*
6 7
1
8
2
9
3
10
4
Department of Laboratory Medicine, University of California, San Francisco, CA, USA 94107
UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA, USA, 914107 Department of Medicine, Division of Infectious Diseases, University of California, San Francisco Department of Internal Medicine, University of California San Francisco, San Francisco, California, USA
11 12
†
These authors contributed equally to this work.
13 14
*Corresponding author
15
Department of Laboratory Medicine
16
University of California San Francisco
17
180 Berry St., Suite 290
18
San Francisco CA 94107
19 20 21
Key words: Rapidly growing mycobacteria, brain abscess, whole genome sequencing
22
Abstract:
23
Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a
24
case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M.
25
immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods,
26
and whole-genome sequencing showed
