Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Uptake and release of alcohol release of their alcohol. Cartilage must be washed sufficiently because of the large amount of alcohol it contains. Drum replacement tissues probably represent a lesser danger to the cochlea because they are not placed near to the oval window or round window. However, tympanic membrane not only washes slowly, but in addition takes up the greatest amount of alcohol. By contrast, preserved temporalis fascia has poor absorptive powers for alcohol and gives it u p very readily. RESUME L‘alcool peut endommager serieusement I’organe cochleaire et il est possible que la surdite resultante d’une complication de tympanoplastie puisse ktre causee par I’alcool laisse dans les matieres homogreffes apres un nettoyage inadequat. On a fait des recherches pour estimer la quantite d’alcool absorbee par les ossicules, le cartilage, le fascia temporal. la dure-mere (dura) et le tympan. On a aussie mesure le degre de rejet d’alcool de ces matieres dans d e la saline au cours du nettoyage. On a trouve que la quantite d’alcool absorbee depend d e la nature d e la matikre. de son poids et de sa superficie. La quantite d‘alcool rejetee par ces matieres varait de rnaniere significative selon la niatiere.
Z USA M M E N F ASSU NG Es ist bekannt. dap Alkohol schwere Cochlearschiiden erzeugen kann und es ist moglich. dafl Taubheit als eine Komplikation von Tympanoplastik verursacht werden kann. wenn Alkohol nach ungenugendem Waschen im Homograftmaterial verbleibt. Es wurde eine Forschung unternommen. in welcher die Menge des Alkohols geschatzt wurde. die von Knocheln, Knorpeln. Temporalis fascia. Dura und tympanalen Memhranen aufgenommen wurde. Es wurde auch die Menge der Alkoholausscheidung von solchen Materialien wiihrend des Waschens in Salzlosung gemessen. Es wurde gefunden. daB die aufge-
303
nommene Menge von der Natur des Materiales. seines Gewichtes und der Oberflache abhing. Die Schnelligkeit der Alkoholausscheidung von diesen Materialien unterschied sich bedeutend fur jedes Material.
REFERENCES Curry, A . S . , Walker, G. W. & Simpson. G . S . 1966. Determination of ethanol in blood by gas chromatography. Analyst 91, 742. Thomas, J . N . & Colman, B. H . 1975. Uptake and release of ethyl alcohol by homograft ossicles and cartilage. J Larvngol 010189, 827. 8.H . C o l m a n , C h . M . , F . R . C . S . Dept. of0tolaryngolog.v The Radrlgfe Infirmary Oxford OX2 6 H E England
DISCUSSION T . Pulvcr: 1) Would it not be better to use the term tissuetoxicity instead of ototoxicity to describe the action of alcohol? 2) I should like to suggest washing your grafts in the future in distilled water which I have been using for many years. We all know from the histological tissue techniques that if y o u want to get rid of alcohol. you wash with distilled water. not with saline. I venture to suggest that if you repeat your experiments with distilled water, you need much shorter time to remove the alcohol. K . SedldEek: Are there any changes in the biological activity of the grafts, caused by alcohol? We observed that the cellular elements of the skin, preserved with alcohol did not grow in tissue culture at all or very slowly. O’Connor (Reply) to Mr Palva: I agree that alcohol has a general tissue toxicity: it is ototoxic as well and the experiment was carried out purely in this specific context. I t may well be safer to wash homografts in water rather than saline: we have not studied the rate of release into water, we should perhaps use water in view of your commen 1. To MrSed1rii;ekc Yes, implant of autograft tissue alway9 carries some risk of adverse reaction, in the cochlea and elsewhere. This experiment however, was purely confined to a study of alcohol in and out of grafts.
Acla Otolaryngol81
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Acta Otolaryngol Downloaded from informahealthcare.com by TIB/UB Hannover on 11/06/14 For personal use only.
Uptake and release of alcohol release of their alcohol. Cartilage must be washed sufficiently because of the large amount of alcohol it contains. Drum replacement tissues probably represent a lesser danger to the cochlea because they are not placed near to the oval window or round window. However, tympanic membrane not only washes slowly, but in addition takes up the greatest amount of alcohol. By contrast, preserved temporalis fascia has poor absorptive powers for alcohol and gives it u p very readily. RESUME L‘alcool peut endommager serieusement I’organe cochleaire et il est possible que la surdite resultante d’une complication de tympanoplastie puisse ktre causee par I’alcool laisse dans les matieres homogreffes apres un nettoyage inadequat. On a fait des recherches pour estimer la quantite d’alcool absorbee par les ossicules, le cartilage, le fascia temporal. la dure-mere (dura) et le tympan. On a aussie mesure le degre de rejet d’alcool de ces matieres dans d e la saline au cours du nettoyage. On a trouve que la quantite d’alcool absorbee depend d e la nature d e la matikre. de son poids et de sa superficie. La quantite d‘alcool rejetee par ces matieres varait de rnaniere significative selon la niatiere.
Z USA M M E N F ASSU NG Es ist bekannt. dap Alkohol schwere Cochlearschiiden erzeugen kann und es ist moglich. dafl Taubheit als eine Komplikation von Tympanoplastik verursacht werden kann. wenn Alkohol nach ungenugendem Waschen im Homograftmaterial verbleibt. Es wurde eine Forschung unternommen. in welcher die Menge des Alkohols geschatzt wurde. die von Knocheln, Knorpeln. Temporalis fascia. Dura und tympanalen Memhranen aufgenommen wurde. Es wurde auch die Menge der Alkoholausscheidung von solchen Materialien wiihrend des Waschens in Salzlosung gemessen. Es wurde gefunden. daB die aufge-
303
nommene Menge von der Natur des Materiales. seines Gewichtes und der Oberflache abhing. Die Schnelligkeit der Alkoholausscheidung von diesen Materialien unterschied sich bedeutend fur jedes Material.
REFERENCES Curry, A . S . , Walker, G. W. & Simpson. G . S . 1966. Determination of ethanol in blood by gas chromatography. Analyst 91, 742. Thomas, J . N . & Colman, B. H . 1975. Uptake and release of ethyl alcohol by homograft ossicles and cartilage. J Larvngol 010189, 827. 8.H . C o l m a n , C h . M . , F . R . C . S . Dept. of0tolaryngolog.v The Radrlgfe Infirmary Oxford OX2 6 H E England
DISCUSSION T . Pulvcr: 1) Would it not be better to use the term tissuetoxicity instead of ototoxicity to describe the action of alcohol? 2) I should like to suggest washing your grafts in the future in distilled water which I have been using for many years. We all know from the histological tissue techniques that if y o u want to get rid of alcohol. you wash with distilled water. not with saline. I venture to suggest that if you repeat your experiments with distilled water, you need much shorter time to remove the alcohol. K . SedldEek: Are there any changes in the biological activity of the grafts, caused by alcohol? We observed that the cellular elements of the skin, preserved with alcohol did not grow in tissue culture at all or very slowly. O’Connor (Reply) to Mr Palva: I agree that alcohol has a general tissue toxicity: it is ototoxic as well and the experiment was carried out purely in this specific context. I t may well be safer to wash homografts in water rather than saline: we have not studied the rate of release into water, we should perhaps use water in view of your commen 1. To MrSed1rii;ekc Yes, implant of autograft tissue alway9 carries some risk of adverse reaction, in the cochlea and elsewhere. This experiment however, was purely confined to a study of alcohol in and out of grafts.
Acla Otolaryngol81
