1601 Open Access Asian Australas. J. Anim. Sci. Vol. 29, No. 11 : 1601-1607 November 2016 http://dx.doi.org/10.5713/ajas.16.0575
www.ajas.info pISSN 1011-2367
eISSN 1976-5517
Use of Lysozyme as a Feed Additive on In vitro Rumen Fermentation and Methane Emission Ashraf A. Biswasa, Sung Sill Lee1,a, Lovelia L. Mamuad, Seon-Ho Kim, Yeon-Jae Choi, Gui-Seck Bae2, Kichoon Lee3, Ha-Guyn Sung4, and Sang-Suk Lee* Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea ABSTRACT: This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane (CH4) production. An in vitro ruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 mg dry matter substrate at different levels of inclusion: Without lysozyme, 2,000, 4,000, and 8,000 U lysozyme. Results revealed that, lysozyme addition had a significant effect on pH after 24 h of incubation, with the highest pH (p
www.ajas.info pISSN 1011-2367
eISSN 1976-5517
Use of Lysozyme as a Feed Additive on In vitro Rumen Fermentation and Methane Emission Ashraf A. Biswasa, Sung Sill Lee1,a, Lovelia L. Mamuad, Seon-Ho Kim, Yeon-Jae Choi, Gui-Seck Bae2, Kichoon Lee3, Ha-Guyn Sung4, and Sang-Suk Lee* Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 540-742, Korea ABSTRACT: This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane (CH4) production. An in vitro ruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 mg dry matter substrate at different levels of inclusion: Without lysozyme, 2,000, 4,000, and 8,000 U lysozyme. Results revealed that, lysozyme addition had a significant effect on pH after 24 h of incubation, with the highest pH (p
