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Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
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Biotech nic 8r Hist o c h e m i5 t r y
Fig. 2. Fresh bull spermatozoa treated with dilauroylphosphatidylcholine liposomes and stained with trypan blue and Giemsa. Bar indicates 10 pm. Fig. 3. Boar spermatozoa washed 2 times, and incubated at 39 C for 6 hr and stained with trypan blue and Giemsa. Bar indicates 10 pm. Fig. 4. Rabbit spermatozoa washed 2 times, and incubated at 39 C for 6 hr and stained with trypan blue and Giemsa Bar indicates 10 pm. Figs. 5 and 6. Frozen-thawed bull spermatozoa treated with calcium ionophore and stained with Congo red and Giemsa. Bar indicates 10 pm. Fig. 7. Fresh bull spermatozoa washed 2 times and incubated at 39 C for 8 hr. Congo red stock solution, phase contrast. Note the live spermatozoon with stained acrosomal region (arrow). Bar indicates 10 pm.
TALP before staining. Neutral red gives a dark precipitate with trypan blue or Congo red, but nothing is stained by neutral red
itself. To evaluate spermatozoa which have been exposed to the commonly used calcium ionophore A 2 3 187, an extra step
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
122
Biotech nic 8r Hist o c h e m i5 t r y
Fig. 2. Fresh bull spermatozoa treated with dilauroylphosphatidylcholine liposomes and stained with trypan blue and Giemsa. Bar indicates 10 pm. Fig. 3. Boar spermatozoa washed 2 times, and incubated at 39 C for 6 hr and stained with trypan blue and Giemsa. Bar indicates 10 pm. Fig. 4. Rabbit spermatozoa washed 2 times, and incubated at 39 C for 6 hr and stained with trypan blue and Giemsa Bar indicates 10 pm. Figs. 5 and 6. Frozen-thawed bull spermatozoa treated with calcium ionophore and stained with Congo red and Giemsa. Bar indicates 10 pm. Fig. 7. Fresh bull spermatozoa washed 2 times and incubated at 39 C for 8 hr. Congo red stock solution, phase contrast. Note the live spermatozoon with stained acrosomal region (arrow). Bar indicates 10 pm.
TALP before staining. Neutral red gives a dark precipitate with trypan blue or Congo red, but nothing is stained by neutral red
itself. To evaluate spermatozoa which have been exposed to the commonly used calcium ionophore A 2 3 187, an extra step
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
Biotech Histochem Downloaded from informahealthcare.com by Universitaets- und Landesbibliothek Duesseldorf on 03/27/13 For personal use only.
