Plant Cell Reports (1992) 11:310-313
Plant Cell Reports 9 Springer-Verlag1992
Visualization of vacuoplasts in isolated vacuole preparations from mesophyll protoplasts of periwinkle [Catharanthus roseus (L.) G. Don] David M c C a s k i l l ~' 2 and A. Ian S c o t t 2
1 Present address: Institute of Biological Chemistry, Washington State University, Pullman, WA., 99164, USA 2 Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas, 77843, USA
Received January 7, 1992/Revised version received March 20, 1992 - Communicated by R. L. Gilbertson
ABSTRACT. A procedure was developed for the rapid detection of vacuoplasts in vacuole preparations isolated from mesophyll protoplasts of Catharanthus roseus (L.) G. Don (periwinkle). The procedure relies on the staining of surface carbohydrates on the plasma membrane surrounding vacuoplasts with fluorescein-labeled lectins. When isolated under conditions of constant osmotic strength, approximately 15-20% of the vacuoles isolated showed surface labeling with FITC-agglutinin from Abrus precatorius. Isolation of vacuoles after an initial osmotic shock showed much lower (
Plant Cell Reports 9 Springer-Verlag1992
Visualization of vacuoplasts in isolated vacuole preparations from mesophyll protoplasts of periwinkle [Catharanthus roseus (L.) G. Don] David M c C a s k i l l ~' 2 and A. Ian S c o t t 2
1 Present address: Institute of Biological Chemistry, Washington State University, Pullman, WA., 99164, USA 2 Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas, 77843, USA
Received January 7, 1992/Revised version received March 20, 1992 - Communicated by R. L. Gilbertson
ABSTRACT. A procedure was developed for the rapid detection of vacuoplasts in vacuole preparations isolated from mesophyll protoplasts of Catharanthus roseus (L.) G. Don (periwinkle). The procedure relies on the staining of surface carbohydrates on the plasma membrane surrounding vacuoplasts with fluorescein-labeled lectins. When isolated under conditions of constant osmotic strength, approximately 15-20% of the vacuoles isolated showed surface labeling with FITC-agglutinin from Abrus precatorius. Isolation of vacuoles after an initial osmotic shock showed much lower (
