S93
Asian Pac J Trop Med 2014; 7(Suppl 1): S93-S96
Contents lists available at ScienceDirect
Asian Pacific Journal of Tropical Medicine journal homepage:www.elsevier.com/locate/apjtm
Document heading
doi: 10.1016/S1995-7645(14)60211-X
variant of DC-SIGN gene promoter associated with resistance to HIV-1 in serodiscordant couples in Burkina Faso
A
1,2
1,3
2
2
4
2
Thérèse Samdapawindé Kagoné , Cyrille Bisseye *, Nicolas Méda , Jean Testa , Virginio Pietra , Dramane Kania 1 1 1 5 1 Théophane Yonli , Tegwindé Rebeca Compaoré , Jean Baptiste Nikiéma , Comlan de Souza , Jacques Simpore
, Albert
Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso
1
Centre MURAZ Bobo-Dioulasso, Burkina Faso
2
Laboratory of Molecular and Cellular Biology, University of Science and Technique of Masuku (USTM), Franceville, Gabon
3
Health Centre Saint Camille of Nanoro, Burkina Faso
4
Faculty of Sciences, University of Lomé, Togo
5
ARTICLE INFO
ABSTRACT
Article history: Received 22 Apr 2014 Received in revised form 9 May 2014 Accepted 18 Jun 2014 Available online 17 Aug 2014
Objective: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5Δ32). Methods: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLAB57*01 and CCR5Δ32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DCSIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5Δ32 deletion was investigated by PCR. Results: The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P
Asian Pac J Trop Med 2014; 7(Suppl 1): S93-S96
Contents lists available at ScienceDirect
Asian Pacific Journal of Tropical Medicine journal homepage:www.elsevier.com/locate/apjtm
Document heading
doi: 10.1016/S1995-7645(14)60211-X
variant of DC-SIGN gene promoter associated with resistance to HIV-1 in serodiscordant couples in Burkina Faso
A
1,2
1,3
2
2
4
2
Thérèse Samdapawindé Kagoné , Cyrille Bisseye *, Nicolas Méda , Jean Testa , Virginio Pietra , Dramane Kania 1 1 1 5 1 Théophane Yonli , Tegwindé Rebeca Compaoré , Jean Baptiste Nikiéma , Comlan de Souza , Jacques Simpore
, Albert
Biomolecular Research Center Pietro Annigoni CERBA/Labiogene University of Ouagadougou, Burkina Faso
1
Centre MURAZ Bobo-Dioulasso, Burkina Faso
2
Laboratory of Molecular and Cellular Biology, University of Science and Technique of Masuku (USTM), Franceville, Gabon
3
Health Centre Saint Camille of Nanoro, Burkina Faso
4
Faculty of Sciences, University of Lomé, Togo
5
ARTICLE INFO
ABSTRACT
Article history: Received 22 Apr 2014 Received in revised form 9 May 2014 Accepted 18 Jun 2014 Available online 17 Aug 2014
Objective: To study the involvement of variations in 4 genes associated with susceptibility and/or protection against HIV-1 in serodiscordant couples in Burkina Faso, namely, genes encoding HLA-B57, interferon regulatory factor 1 (IRF1), dendritic cell-specific ICAM3-grabbing nonintegrin (DC-SIGN) and CCR5 delta 32 (CCR5Δ32). Methods: Two DC-SIGN and two IRF1 single nucleotide polymorphisms (SNPs) as well as HLAB57*01 and CCR5Δ32 alleles were genotyped in 51 serodiscordant couples in Burkina Faso. DCSIGN, IRF1 and HLA-B57*01 genotyping was carried out by real time PCR using TaqMan assays (Applied Biosystems, USA and Sacace Biotechnologies, Italy). CCR5Δ32 deletion was investigated by PCR. Results: The two SNPs of DC-SIGN promoter showed a significant genotypic difference in serodiscordant couples. After multivariate analysis, only the association between DC-SIGN rs2287886 and HIV-1 remained significant (P
