This article was downloaded by: [New York University] On: 09 January 2015, At: 10:29 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
Preparative Biochemistry Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/lpbb19
Purification of Adenosine Deaminase from ChickenEgg Yolk by Affinity Column Chromatography a
a
a
R. Lopez , F. Cabre , R. Franco , H. a
Cascante & E. I. Canela
a
a
Department of Biochemistry and Physiology, Faculty of Chemistry , University of Barcelona , Martí i Franquès, 1, 08071, Barcelona, Catalonia, Spain Published online: 23 Oct 2006.
To cite this article: R. Lopez , F. Cabre , R. Franco , H. Cascante & E. I. Canela (1990) Purification of Adenosine Deaminase from Chicken-Egg Yolk by Affinity Column Chromatography, Preparative Biochemistry, 20:3-4, 199-204, DOI: 10.1080/00327489008050196 To link to this article: http://dx.doi.org/10.1080/00327489008050196
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings,
demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content.
Downloaded by [New York University] at 10:29 09 January 2015
This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/terms-andconditions
PREPARATIVE BIOCHEMISTRY, 2 0 ( 3 & 4 ) , 1 9 9 - 2 0 4 ( 1 9 9 0 )
PURIFICATION OF AD6NOSINB D W I N A S E FROM CHICKEN-EGQ YOLK
BY AFFINITY COLUEM CHROMATOGRAPHY
Downloaded by [New York University] at 10:29 09 January 2015
R. Lopez, F. Cabre, €7. Franco, M. Cascante and E. I. W. Department of
Biochemistry
and Physiology.
Faculty of
Chemistry.
University of Barcelona. Marti i FranquBs, 1. 08071-Barcelona. Catalonia. Spain.
Adenosine deaminase (adenosine aminohydrolase; E.C. 3.5.4.4) has been purified 4686-fold from egg yolk. The procedure developed was used to isolate the enzyme from eight chicken eggs. An easily prepared affinity column employing purine riboside was used as the final step in the purification. The method developed permits the rapid isolation and a high recovery of the protein The specific activity of the enzyme preparation obtained is 81.4 mU/mg.
.
INTRODUCTION
Adenosine deaminase (adenosine aminohydrolase E.C. been studied from a wide variety of
source^'^^.
adenosine deaminase has been
studied due
poorly
3.5.4.4.)
has
However, egg yolk to purification
difficulties. So, only De Boeck et al.4 and Suzuki et a15 have described methods to purify the enzyme. The method of De Boeck et al.4, based on gel permeation chromatography, provides better results than the method described by Suzuki et ale5, based on both gel permeation and ionic exchange chromatography. However, both methods yield non-heterogeneous
199 Copyright 0 1990 by Marcel Dekker, Inc.
2 00
LOPEZ ET AL.
final protein preparations of egg yolk adenosine deaminase. In spite of
the
interest of elucidating
the characteristics of
egg
yolk
adenosine deaminase in order to understand the metabolism of nucleic acids in chicken6-? and
few studies have been performed with
this enzyme. Purification of the enzyme is difficult for two reasons: a) The high lipid content of egg yolk (30%) and b) The low activity of the enzyme present in egg yolk.
Downloaded by [New York University] at 10:29 09 January 2015
In this study we present a quick method for purifying adenosine deaminase
from
egg
yolk
based
on
affinity
chromatography.
This
procedure yields preparations of the enzyme with higher specific activity and higher recovery than those described to date.
MATERIALS AND METHODS
Materials Sephadex and epoxy activated-Sepharose 68 gels were from Pharmacia Fine Chemicals. Biochemical reagents used for enzyme purification and assay were of reagent grade or higher. Unfertilized white eggs 1-5 days old, were used and the isolation procedure started immediately after the separation of yolk and white.
Methods Adenosine deaminase activity.- Adenosine deaminase activity was determined according to the method of Gustin and Kemp”.
A unit (U) is
defined as the amount of enzyme that liberates 1 pmol NH3 per min from adenosine in the given conditions.
Protein.- Protein in the enzyme solutions was determined by the method of Lowry et a1.12 using bovine serum albumin as standard.
Preparation
of
purine
riboside-epoxy activated
Sepharose
60
affinity-gel (PRAQ) column.- The ligand purine riboside was coupled to epoxy-activated Sepharose 6B by the method of Schrader et a1.13, but purine riboside was was used instead of adenosine14.
PURIFICATION OF ADENOSINE DEAMINASE
201
RESULTS
step 1.- Preparation of Initial Extract. Yolk was diluted with an equal volume of distilled water and gently mixed for five minutes. The mixture was centrifuged for 30 minutes at 31,300 x g and 2OC. The pellet was discarded.
Downloaded by [New York University] at 10:29 09 January 2015
Step 2.- Ether Delipidation. NaCl was added to the supernatant to make a final concentration of 0.3 M. Delipidation was carried out by extraction with 1.5 volumes of peroxide-free
ether. The aqueous phase was separated from the
organic phase by centrifugation at 6000 x g for 10 minutes.
Step 3.- Acetone Powder. With stirring the aqueous phase acetone at -18 OC was slowly added until a
final concentration of 608
(v/v) was reached.
The
suspension was centrifuged at 15000 x g for 15 min at -1OOC. A volume of phosphate buffer 50 mM,
pH 1.2, equal to the initial volume of
aqueous phase was used to resuspend the pellet.
Step 4.- Ammonium Sulfate Fractionation. Ammonium sulfate was added to the resuspended acetone pellet to give a 35% saturated solution. The suspension was stirred in an ice bath for 30 min and then centrifuged at 25,300 x g for 30 min at 5 OC. The pellet had no activity and was discarded.
Step 5.- Senhadex 0-25 Chromatoaraphy. The supernatant was filtered through a Sephadex G-25 column equilibrated with 25 mM phosphate buffer, pH 7.2, and eluted with the same buffer.
Stem 6.- Purine Ribosi.de Senharose 6B Affinity
Chromatoaraphv.
The pooled active eluates were applied to a riboside Sepharose 6B column (1.6 cm x 7 cm) equilibrated with 25 mM phosphate buffer, pH 7.2, containing 2-mercaptoetanol 1 mM and sodium azide 0.02% at a flow
202
LOPEZ ET AL. TABLE I
Purification and Recoveries of Adenosine Deaminase of Egg Yolk.
Downloaded by [New York University] at 10:29 09 January 2015
Fraction
Activity (mu)
Protein (mg)
Yield
S.A.
G.P
(mu/mg)
Initial Supernatant
131.2
7551
0.0174
100%
1
After ether extraction
121.1
7266
0.0166
92%
0.96
Acetone powder
84.7
1852
0.0457
64%
2.6
Ammonium sulfate ftact ionat ion
72.0
1369
0.0526
54%
3.0
Sephadex G-25
57.7
851
0.0678
44%
3.9
Affinity Chromatography
28.5
0.349
81.4
22%
4687
rate of 6 ml/h. After the sample had been applied, the column was washed with 32 ml of the equilibration buffer. Adenosine deaminase activity was eluted with the equilibration buffer containing 1M sodium chloride at a 6 ml/h flow rate. The results of a complete purification of the egg yolk adenosine deaminase from eight eggs are summarized in Table 1. The yield was 21.7% and the final preparation had a specific activity of 81.4 U/mg, which corresponds to a purification factor of 4687 fold with respect to the supernatant of the first egg yolk extract.
The purification method presented in this paper is suitable for the isolation of egg yolk adenosine deaminase. One of the advantages of the method proposed is its speed. The process should be completed in approximately 40 hours.
203
PURIFICATION OF ADENOSINE DEAMINASE
Comparison of the procedure used here with those developed by De Boeck et a14 and Suzuki et a15 for the enzyme from egg yolk, shows that the method reported in this paper gives both higher final specific activity and higher yield. The main difference is the use of affinity chromatography
instead
of
gel
filtration
or
ionic
exchange
chromatography, which increases the degree of purification more than 1000 fold in a single step. Purine riboeide affinity chromatography
Downloaded by [New York University] at 10:29 09 January 2015
has been described as useful to purify adenosine deaminase from calf intestinal mucosa, frog liver and scallop adductor muscle14 and from
erythrocyte^'^.
In the present work we use this chromatography as the
final step to purify the egg yolk enzyme; this leads to a high specific activity in one step.
1.- P.F. Ma and J.R. Fisher, Comp. Biochem. Physiol. 27,
105-112,
(1968).
2.- M.B. Van der Weyden and W.N. Kelley J.Biol. Chem. 251, 5448-5456, (1976). 3.- P.E. Daddona, D.S. Shewack, W.N. Kelley, P. Argos, A.F Markhan and
S.H. Orkin, J. Biol. Chem. 259, 12101-12106, (1984). 4.- S. De Boeck, T. Rymen and J. Stockx, Eur. J. Biochem.
52, 191-195,
(1975). 5.- H. Suzuki, H. Takahashi, K. Ikeda
and K. Kohsida, Bulletin of
Science of Engeneering Laboratory Waseda University. 102, 23-29, (1982). 6.- P.F. Ma and J.R. Fisher, Biochim. Biophys. Acta. 159, 153-159, (1968). 7.- V.D.
Hoagland and J.R. Fisher, J. Biol. Chem.
242,
4341-4351,
(1967). 8.- A. Moors and J. Stockx, Arch. Int. Phyeiol. Biochim.
a,712-732,
(1972). 9.- A. Moors and J. Stockx, Arch. Int. Physiol. Biochim. &, 923-934,
(1972). 10.- S. De Boeck and J. Stockx, Arch. Int. Phyeiol. Biochim. 959, (1972).
a,957-
2 04 11.-
LOPEZ ET AL. N.C.
Gustin and R.G.
Kemp, Analytical Biochem.
21,
527-532,
(1976). 12.- O.H. Chem.
Lowry, N.J.
Rosebrough, A.L.
Farr and R.J.
Randal, J. Biol.
193, 265-275, (1951).
13.- W.P.
Schrader, A.R.
Stacy and B. Pollara, J. Biol. Chem. 251,
4026-4032 I (1976). 14.- T. Ogawa, Y. Aikawa and T. Aikawa, Comp. Biochem. Physiol. M I
Downloaded by [New York University] at 10:29 09 January 2015
491-495, (1987). 15.- J.M. Aran and R. Franco, J. Chromat. submitted (1990).
Preparative Biochemistry Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/lpbb19
Purification of Adenosine Deaminase from ChickenEgg Yolk by Affinity Column Chromatography a
a
a
R. Lopez , F. Cabre , R. Franco , H. a
Cascante & E. I. Canela
a
a
Department of Biochemistry and Physiology, Faculty of Chemistry , University of Barcelona , Martí i Franquès, 1, 08071, Barcelona, Catalonia, Spain Published online: 23 Oct 2006.
To cite this article: R. Lopez , F. Cabre , R. Franco , H. Cascante & E. I. Canela (1990) Purification of Adenosine Deaminase from Chicken-Egg Yolk by Affinity Column Chromatography, Preparative Biochemistry, 20:3-4, 199-204, DOI: 10.1080/00327489008050196 To link to this article: http://dx.doi.org/10.1080/00327489008050196
PLEASE SCROLL DOWN FOR ARTICLE Taylor & Francis makes every effort to ensure the accuracy of all the information (the “Content”) contained in the publications on our platform. However, Taylor & Francis, our agents, and our licensors make no representations or warranties whatsoever as to the accuracy, completeness, or suitability for any purpose of the Content. Any opinions and views expressed in this publication are the opinions and views of the authors, and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings,
demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content.
Downloaded by [New York University] at 10:29 09 January 2015
This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden. Terms & Conditions of access and use can be found at http://www.tandfonline.com/page/terms-andconditions
PREPARATIVE BIOCHEMISTRY, 2 0 ( 3 & 4 ) , 1 9 9 - 2 0 4 ( 1 9 9 0 )
PURIFICATION OF AD6NOSINB D W I N A S E FROM CHICKEN-EGQ YOLK
BY AFFINITY COLUEM CHROMATOGRAPHY
Downloaded by [New York University] at 10:29 09 January 2015
R. Lopez, F. Cabre, €7. Franco, M. Cascante and E. I. W. Department of
Biochemistry
and Physiology.
Faculty of
Chemistry.
University of Barcelona. Marti i FranquBs, 1. 08071-Barcelona. Catalonia. Spain.
Adenosine deaminase (adenosine aminohydrolase; E.C. 3.5.4.4) has been purified 4686-fold from egg yolk. The procedure developed was used to isolate the enzyme from eight chicken eggs. An easily prepared affinity column employing purine riboside was used as the final step in the purification. The method developed permits the rapid isolation and a high recovery of the protein The specific activity of the enzyme preparation obtained is 81.4 mU/mg.
.
INTRODUCTION
Adenosine deaminase (adenosine aminohydrolase E.C. been studied from a wide variety of
source^'^^.
adenosine deaminase has been
studied due
poorly
3.5.4.4.)
has
However, egg yolk to purification
difficulties. So, only De Boeck et al.4 and Suzuki et a15 have described methods to purify the enzyme. The method of De Boeck et al.4, based on gel permeation chromatography, provides better results than the method described by Suzuki et ale5, based on both gel permeation and ionic exchange chromatography. However, both methods yield non-heterogeneous
199 Copyright 0 1990 by Marcel Dekker, Inc.
2 00
LOPEZ ET AL.
final protein preparations of egg yolk adenosine deaminase. In spite of
the
interest of elucidating
the characteristics of
egg
yolk
adenosine deaminase in order to understand the metabolism of nucleic acids in chicken6-? and
few studies have been performed with
this enzyme. Purification of the enzyme is difficult for two reasons: a) The high lipid content of egg yolk (30%) and b) The low activity of the enzyme present in egg yolk.
Downloaded by [New York University] at 10:29 09 January 2015
In this study we present a quick method for purifying adenosine deaminase
from
egg
yolk
based
on
affinity
chromatography.
This
procedure yields preparations of the enzyme with higher specific activity and higher recovery than those described to date.
MATERIALS AND METHODS
Materials Sephadex and epoxy activated-Sepharose 68 gels were from Pharmacia Fine Chemicals. Biochemical reagents used for enzyme purification and assay were of reagent grade or higher. Unfertilized white eggs 1-5 days old, were used and the isolation procedure started immediately after the separation of yolk and white.
Methods Adenosine deaminase activity.- Adenosine deaminase activity was determined according to the method of Gustin and Kemp”.
A unit (U) is
defined as the amount of enzyme that liberates 1 pmol NH3 per min from adenosine in the given conditions.
Protein.- Protein in the enzyme solutions was determined by the method of Lowry et a1.12 using bovine serum albumin as standard.
Preparation
of
purine
riboside-epoxy activated
Sepharose
60
affinity-gel (PRAQ) column.- The ligand purine riboside was coupled to epoxy-activated Sepharose 6B by the method of Schrader et a1.13, but purine riboside was was used instead of adenosine14.
PURIFICATION OF ADENOSINE DEAMINASE
201
RESULTS
step 1.- Preparation of Initial Extract. Yolk was diluted with an equal volume of distilled water and gently mixed for five minutes. The mixture was centrifuged for 30 minutes at 31,300 x g and 2OC. The pellet was discarded.
Downloaded by [New York University] at 10:29 09 January 2015
Step 2.- Ether Delipidation. NaCl was added to the supernatant to make a final concentration of 0.3 M. Delipidation was carried out by extraction with 1.5 volumes of peroxide-free
ether. The aqueous phase was separated from the
organic phase by centrifugation at 6000 x g for 10 minutes.
Step 3.- Acetone Powder. With stirring the aqueous phase acetone at -18 OC was slowly added until a
final concentration of 608
(v/v) was reached.
The
suspension was centrifuged at 15000 x g for 15 min at -1OOC. A volume of phosphate buffer 50 mM,
pH 1.2, equal to the initial volume of
aqueous phase was used to resuspend the pellet.
Step 4.- Ammonium Sulfate Fractionation. Ammonium sulfate was added to the resuspended acetone pellet to give a 35% saturated solution. The suspension was stirred in an ice bath for 30 min and then centrifuged at 25,300 x g for 30 min at 5 OC. The pellet had no activity and was discarded.
Step 5.- Senhadex 0-25 Chromatoaraphy. The supernatant was filtered through a Sephadex G-25 column equilibrated with 25 mM phosphate buffer, pH 7.2, and eluted with the same buffer.
Stem 6.- Purine Ribosi.de Senharose 6B Affinity
Chromatoaraphv.
The pooled active eluates were applied to a riboside Sepharose 6B column (1.6 cm x 7 cm) equilibrated with 25 mM phosphate buffer, pH 7.2, containing 2-mercaptoetanol 1 mM and sodium azide 0.02% at a flow
202
LOPEZ ET AL. TABLE I
Purification and Recoveries of Adenosine Deaminase of Egg Yolk.
Downloaded by [New York University] at 10:29 09 January 2015
Fraction
Activity (mu)
Protein (mg)
Yield
S.A.
G.P
(mu/mg)
Initial Supernatant
131.2
7551
0.0174
100%
1
After ether extraction
121.1
7266
0.0166
92%
0.96
Acetone powder
84.7
1852
0.0457
64%
2.6
Ammonium sulfate ftact ionat ion
72.0
1369
0.0526
54%
3.0
Sephadex G-25
57.7
851
0.0678
44%
3.9
Affinity Chromatography
28.5
0.349
81.4
22%
4687
rate of 6 ml/h. After the sample had been applied, the column was washed with 32 ml of the equilibration buffer. Adenosine deaminase activity was eluted with the equilibration buffer containing 1M sodium chloride at a 6 ml/h flow rate. The results of a complete purification of the egg yolk adenosine deaminase from eight eggs are summarized in Table 1. The yield was 21.7% and the final preparation had a specific activity of 81.4 U/mg, which corresponds to a purification factor of 4687 fold with respect to the supernatant of the first egg yolk extract.
The purification method presented in this paper is suitable for the isolation of egg yolk adenosine deaminase. One of the advantages of the method proposed is its speed. The process should be completed in approximately 40 hours.
203
PURIFICATION OF ADENOSINE DEAMINASE
Comparison of the procedure used here with those developed by De Boeck et a14 and Suzuki et a15 for the enzyme from egg yolk, shows that the method reported in this paper gives both higher final specific activity and higher yield. The main difference is the use of affinity chromatography
instead
of
gel
filtration
or
ionic
exchange
chromatography, which increases the degree of purification more than 1000 fold in a single step. Purine riboeide affinity chromatography
Downloaded by [New York University] at 10:29 09 January 2015
has been described as useful to purify adenosine deaminase from calf intestinal mucosa, frog liver and scallop adductor muscle14 and from
erythrocyte^'^.
In the present work we use this chromatography as the
final step to purify the egg yolk enzyme; this leads to a high specific activity in one step.
1.- P.F. Ma and J.R. Fisher, Comp. Biochem. Physiol. 27,
105-112,
(1968).
2.- M.B. Van der Weyden and W.N. Kelley J.Biol. Chem. 251, 5448-5456, (1976). 3.- P.E. Daddona, D.S. Shewack, W.N. Kelley, P. Argos, A.F Markhan and
S.H. Orkin, J. Biol. Chem. 259, 12101-12106, (1984). 4.- S. De Boeck, T. Rymen and J. Stockx, Eur. J. Biochem.
52, 191-195,
(1975). 5.- H. Suzuki, H. Takahashi, K. Ikeda
and K. Kohsida, Bulletin of
Science of Engeneering Laboratory Waseda University. 102, 23-29, (1982). 6.- P.F. Ma and J.R. Fisher, Biochim. Biophys. Acta. 159, 153-159, (1968). 7.- V.D.
Hoagland and J.R. Fisher, J. Biol. Chem.
242,
4341-4351,
(1967). 8.- A. Moors and J. Stockx, Arch. Int. Phyeiol. Biochim.
a,712-732,
(1972). 9.- A. Moors and J. Stockx, Arch. Int. Physiol. Biochim. &, 923-934,
(1972). 10.- S. De Boeck and J. Stockx, Arch. Int. Phyeiol. Biochim. 959, (1972).
a,957-
2 04 11.-
LOPEZ ET AL. N.C.
Gustin and R.G.
Kemp, Analytical Biochem.
21,
527-532,
(1976). 12.- O.H. Chem.
Lowry, N.J.
Rosebrough, A.L.
Farr and R.J.
Randal, J. Biol.
193, 265-275, (1951).
13.- W.P.
Schrader, A.R.
Stacy and B. Pollara, J. Biol. Chem. 251,
4026-4032 I (1976). 14.- T. Ogawa, Y. Aikawa and T. Aikawa, Comp. Biochem. Physiol. M I
Downloaded by [New York University] at 10:29 09 January 2015
491-495, (1987). 15.- J.M. Aran and R. Franco, J. Chromat. submitted (1990).
